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Priyanka Thakur
Vinay Pandit


Diabetes, Atropa acuminata, antioxidant, HPTLC, Lipid peroxidation, alphaamylase


Objective: The purpose of this study was to look into the antidiabetic and antioxidant properties of hydroethanolicextract of Atropa acuminata (HEEAA). Experimental research was conducted to furnish the scientific evidence of antioxidant activity against various stable free radicals like nitric oxide, superoxide, and lipid peroxidation (measures the radical scavenging activity of antioxidants) and ant diabetic potential against different enzymes like alpha-amylase, alphaglycosidase, dipeptidyl peptidase IV (DPP-IV), pancreatic lipase,etc. of Atropa plant (Atropa acuminata)and also evaluate their maximum inhibitory value (IC50 values).

Methods: Shade-dried root material was pulverized, homogenous material was passed through sieve number 40, using solvent ethanol: water in a ratio of 50:50 soxhletextraction was performed. Thick semi-solid material was vacuum dried using a rotary vacuum flash evaporator and then concentrated in a desiccator, stored in a refrigerator at 40°C for evaluating maximum inhibitory concentration (IC50 values) in both activities (free radical scavenging and enzyme inhibitory antidiabetic activity).


Results: High-performance thin layer chromatography (HPTLC) results confirmed the presence of atropine in the extract withan RF valueof 0.37 and the percentage yield calculated as per standard calculation for atropine was 0.553 % w/v respectively. Among the different antioxidant studies, the maximum inhibitory concentration value in lipid peroxidation assay for plant extract was greater (170.3±0.6 and 152.4±0.5) in comparison to other antioxidant assays performed. Although,the extract showed no or very weak significant inhibition against dipeptidyl peptidase IV and pancreatic lipase.The extract displayed an appreciable effect on alpha-amylase and alphaglycosidase enzyme inhibition at the highest investigated concentration (200 µg/ml).

Conclusion: The results showed that A. acuminata hydroethanolic extract has anti-oxidant activity and appears to exert a hypoglycemic impact.

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