Comparative evaluation of Apical Microleakage of Bioceramic sealer versus Bioceramic sealer mixed with Chitosan nanoparticles – An In-Vitro Study.
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Abstract
The successful outcome of the treatment depends upon good apical seal which will prevent microleakage. The goal of this study was to assess the addition of chitosan nanoparticles to Bioceramic sealer's apical sealing capacity. A total of 20 single rooted single canal teeth were used. The root canals were prepared using rotary files up to #30/.06 and irrigated with 2.5% NaOCl and normal saline. Samples were divided into 2 groups. Group I was obturated with gutta-percha using Bioceramic sealer and Group II was obturated with gutta-percha using Bioceramic-Chitosan-nanoparticles sealer. All samples were immersed in methylene blue 2% solution. The specimens were sectioned, observed under stereomicroscope and the dye leakage was measured. The data were analysed. The results of Unpaired t-test/Independent samples t-test showed that mean microleakage is significantly lower in group II as compared to group I. This study concludes that Chitosan nanoparticles incorporation improves the efficacy of Bioceramic sealer.
References
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22. Brandao C, De Moraes I, Bramante C. Apical sealing ability of ionomeric endodontic sealers. Rev FOB. 2001;9:29-34.
23. Kersten HW, Moorer WR. Particles and molecules in endodontic leakage. Int Endod J. 1989;22:118-24.
24. Ahlberg KM, Assavanop P, Tay WM. A comparison of the apical dye penetration patterns shown by methylene blue and india ink in root-filled teeth. Int Endod J. 1995;28:30-4.
25. Verissimo DM, do Vale MS. Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J Oral Sci. 2006;48:93-8.
26. Almeida JF, Gomes BP, Ferraz CC, Souza-Filho FJ, Zaia AA. Filling of artificial lateral canals and microleakage and flow of five endodontic sealers. Int Endod J. 2007;40:692-9
27. Desouky AA, Negm MM, Ali MM. Sealability of different root canal nanosealers: Nano calcium hydroxide and nano bioactive glass. Open Dent J 2019;13:308-15.
28. Ratih DN, Enggardipta RA, Kusumo ANH, Hadriyanto W. Setting time, flowability, and solubility of epoxy resin-based sealer mixed with chitosan nanoparticles. Int j app pharm. 2021 mar;13(2):122-6.
2. Koch K, Brave D. Bioceramic technology: the game changer in endodontics. Endodontic Practice 2009;2:17–21.
3. Candeiro GT, Correia FC, Duarte MA, Ribeiro-Siqueira DC, Gavini G. Evaluation of radiopacity, pH, release of calcium ions, and flow of a bioceramic root canal sealer. J Endod. 2012 Jun;38(6):842-5.
4. Yehia NM, Al-Ashry S, Hashem A, Nabeel M. Evaluation of Antimicrobial Efficacy and Adaptability to Root Canal Dentin of Bioceramic Sealer Containing Nanoparticles (In-vitro Study). Journal of Fundamental and Clinical Research. 2022 Jun 1;2(1):56-73.
5. Bonde K, Beri L, Bhargava K, Mulay S, Mastud S, Kumar T. Comparative Evaluation of The Antibacterial and Physicochemical Properties Of Bioceramic Apexit Plus Sealer Mixed With Cationic Nanoparticles-In Vitro Study. Pravara Med Rev 2019 Jun 1;11(2):40-.4.
6. Ratih DN, Sari NI, Santosa P, Kaswati NMN. Time-Dependent Effect of Chitosan Nanoparticles as Final Irrigation on the Apical Sealing Ability and Push-Out Bond Strength of Root Canal Obturation. Int J Dent. 2020 Jul 15;2020:8887593.
7. Muliyar S, Shameem KA, Thankachan RP, Francis PG, Jayapalan CS, Hafiz KA. Microleakage in endodontics. J Int Oral Health. 2014 Nov-Dec;6(6):99-104.
8. Grossman LI. Endodontic Practice, 10th ed. Philadelphia:Henry Kimpton Publishers; 1981:297.
9. Komabayashi T, Colmenar D, Cvach N, Bhat A, Primus C, Imai Y. Comprehensive review of current endodontic sealers. Dent Mater J. 2020 Sep 29;39(5):703-20.
10. Salz U, Poppe D, Sbicego S, Roulet JF. Sealing properties of a new root canal sealer. Int Endod J 2009; 42: 1084-9.
11. Jardine AP, Rosa RA, Santini MF, Wagner M, So MV, Kuga MC, et al. The effect of final irrigation on the penetrability of an epoxy resin-based sealer into dentinal tubules: a confocal microscopy study. Clin Oral Investig 2016; 20: 117-23.
12. Coronal leakage – Clinical and biological implications in endodontic success. Endodontics Colleagues for Excellence 2002: 2-7.
13. Kim SY, Kim KJ, Yi YA, Seo DG. Quantitative microleakage analysis of root canal filling materials in single-rooted canals. Scanning 2015; 37: 237-45.
14. Twincy J, Joy M, Joseph J, Krishnan H, Basil J, "Bioceramics as Root Canal Sealers: A Review", International Journal of Science and Research (IJSR), Volume 9 Issue 11, November 2020, pp. 494-8.
15. Donnermeyer D, Dornseifer P, Schäfer E, Dam - maschke T. The push-out bond strength of calcium silicate-based endodontic sealers. Head Face Med. 2018;14:1-7.
16. Kim H, Kim E, Lee S-J, Shin S-J. Comparisons of the Retreatment Efficacy of Calcium Silicate and Epoxy Resin–based Sealers and Residual Sealer in Dentinal Tubules. J Endod. 2015;41: 2025-30.
17. Sanap P, Hegde V, Ghunawat D, Patil M, Nagaonkar N, Jagtap V. Current applications of chitosan nanoparticles in dentistry: A review. Int J Appl Dent Sci. 2020;6(4):81-4.
18. Shetty C, Shetty A, Shetty S, Kaur G, Hedge MN, Nidhi L. Applications of chitosan in dentistry. Indian J Public Health Res Dev 2020;11(3):89-95.
19. Bernkop-Schnürch A. Chitosan and its derivatives: potential excipients for peroral peptide delivery systems. Int J Pharm. 2000 Jan 20;194(1):1-13.
20. Darrag AM. Effectiveness of different final irigation solutions on smear layer removal in intraradicular dentin. Tanta Dent J. 2014; 11:93-9.
21. Jafari F, Jafari S. Importance and methodologies of endodontic microleak- . Importance and methodologies of endodontic microleakage studies: A systematic review. J Clin Exp Dent. 2017;9(6):e812-9.
22. Brandao C, De Moraes I, Bramante C. Apical sealing ability of ionomeric endodontic sealers. Rev FOB. 2001;9:29-34.
23. Kersten HW, Moorer WR. Particles and molecules in endodontic leakage. Int Endod J. 1989;22:118-24.
24. Ahlberg KM, Assavanop P, Tay WM. A comparison of the apical dye penetration patterns shown by methylene blue and india ink in root-filled teeth. Int Endod J. 1995;28:30-4.
25. Verissimo DM, do Vale MS. Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J Oral Sci. 2006;48:93-8.
26. Almeida JF, Gomes BP, Ferraz CC, Souza-Filho FJ, Zaia AA. Filling of artificial lateral canals and microleakage and flow of five endodontic sealers. Int Endod J. 2007;40:692-9
27. Desouky AA, Negm MM, Ali MM. Sealability of different root canal nanosealers: Nano calcium hydroxide and nano bioactive glass. Open Dent J 2019;13:308-15.
28. Ratih DN, Enggardipta RA, Kusumo ANH, Hadriyanto W. Setting time, flowability, and solubility of epoxy resin-based sealer mixed with chitosan nanoparticles. Int j app pharm. 2021 mar;13(2):122-6.
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Published
Oct 14, 2023
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Dr. Sushmita Rane, Dr. Varsha Pandit, Dr. Ashwini Gaikwad, Dr. Mrunal Shinde, Dr. Shivani Chavan, Dr. Abhijit Jadhav. (2023). Comparative evaluation of Apical Microleakage of Bioceramic sealer versus Bioceramic sealer mixed with Chitosan nanoparticles – An In-Vitro Study. Journal of Population Therapeutics and Clinical Pharmacology, 30(18), 179–188. https://doi.org/10.53555/jptcp.v30i18.3136
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All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.