IN VITRO ANTIFUNGAL ACTIVITY OF SWINGLEA GLUTINOSA LEAF EXTRACT AGAINST COLLETOTRICHUM GLOEOSPORIOIDES ON PERSEA AMERICANA MILL

Main Article Content

Pavel Peroza-Piñere
Donicer E. Montes Vergara
Alexander Pérez Cordero

Keywords

phytopathogens, bioproducts, anthracnose, avocado, Colletotrichum, gloeosporioides

Abstract

The avocado (Persea americana Mill.) is a species whose cultivation is of great nutritional and economic importance for the Maria mounts region, the variety of avocado grown is the Antillean race and var. lorena; however, like any other crop, it is often affected by pests and diseases that limit its commercialization at national and world level. The phytopathogenic fungus Colletotrichum gloeosporioides is the causal agent of anthracnose in avocado and manifests itself in the early stages of fruit development, as well as in postharvest and during storage, under conditions of high relative humidity (80%) and temperatures from 20 ◦C. Economic losses due to this fungus can be up to 20% of production.   Therefore, the objective of this work was to evaluate the antifungal activity of extracts and fractions of Swinglea glutinosa against Colletotrichum gloeosporioides, in avocado crop The plant material used corresponded to dried leaves of S. glutinosa collected in two agroecological regions of the department of Sucre, Colombia. The collected plant material was used to obtain ethanolic extracts. Three chromatographic fractions were obtained from the extracts and four concentrations (1%, 25%, 50% and 75%) were prepared per municipality. Each concentration was used for In vitro evaluation against C. gloeosporioides. The results obtained show that at a concentration of 75%, the highest percentages of antifungal index (% A.I.) were obtained for the bioproduct fraction 2 and extract from the municipality of Sincelejo with a % A.I. of 96.6% and 87.2%, respectively.  In this study, both extracts and fractions 2 showed 100% inhibition in the germination of C. gloeosporioides conidia at a concentration of 25%. The chromatographic profile of the bioproducts found evidenced the presence of terpenoid compounds, in which antimicrobial activity has been demonstrated.

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References

1. Bueno-Sánchez J. G., Martínez-Morales J. R., Stashenko E. E., & Ribón, W. (2009): Anti-tubercular activity of eleven aromatic and medicinal plants occurring in Colombia. Biomedica, 29: 51-60.
2. Camargo Piñeres Y., Zambrano Montenegro G., Ortega-Cuadros M., Gutierrez Montero, D. J., & Yepes, J. A. (2021): Actividad antifúngica in vitro del aceite esencial de Swinglea glutinosa Merr sobre Colletotrichum sp. patógeno de mango (Mangifera indica L). Revista Colombiana de Biotecnología, 23: 62-71.
3. García-Mateos, M. R., Acosta-Ramos, M., Rodríguez-Pérez, E., Vásquez-Sánchez, J., & Hernández-Ramos, L. (2021). Extractos vegetales para el control de Colletotrichum gloeosporioides in vitro, en periodo de floración y poscosecha del fruto de Carica papaya. Polibotanica, 51: 213-228.
4. Alarcón R. J., Chavarriaga M. (2007): Diagnóstico precoz de la antracnosis (Colletotrichum gloeosporioides) (Penz) Penz and Sacc. En tomate de árbol mediante el empleo de infecciones quiescentes. Agron, 15: 89-102.
5. Burt, S. (2004): Essential oils: their antibacterial properties and potential applications in foods-A review. Int. J. Food Microbiol, 94: 223–253.
6. Burt S.A., Van Der Zee, R., Koets, A.P., de Graaff, Van Knapen, F., Gaastra, W., Haagsman, H.P., Veldhuizen, E.J. (2007): Carvacrol induces heat shock protein and inhibits synthesis of flagellin in Escherichia coli O157:H7. Appl. Environ. Microbiol, 73: 4484–4490.
7. Consentin S., Tuberoso,C.I., Pisano B., Satta M., Mascia V., Arzedi E., Palmas F. (1994): In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Lett Appl Microbio, 29:130-135.
8. Cowan M. (1999): Plant products as antimicrobial agents. clin. microbiol. rev. 10: 564-582.
9. Edris A.E. (2007): Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytother, 21:308-323.
10. Espitia-Baena J.E., Robledo-Restrepo S.M., Cuadrado-Cano B.S., Duran-Sandoval H.R., Gómez-Estrada H.A. (2014): Perfil fitoquímico, actividad anti-Leishmania, hemolítica y toxicológica de Cordia dentata Poir. y Heliotropium indicum L. Revista Cubana de Plantas Medicinales, 9: 1-21.
11. Stashenko E., Martínez J. R., Medina J. D., & Durán C. (2015): Analysis of essential oils isolated by steam distillation from Swinglea glutinosa fruits and leaves. Journal of Essential Oil Research, 27: 276-282.
12. Gil A. I., Celis Á., & Cuevas J. C. (2010): Efecto inhibitorio de extractos de Swinglea glutinosa (Blanco) Merr. y Lantana camara L. en preemergencia y posemergencia. Revista Colombiana de Ciencias Hortícolas, 4: 223-234.
13. Hincapié O. D., Saldarriaga A., Díaz, C. (2017): Biological, botanical and chemical alternatives for the control of blackberry (Rubus glaucus Benth.) diseases. Revista Facultad Nacional de Agronomía, 70: 8169- 8176.
14. Hurtado E., Fernández A., Carrasco A. (2018): Avocado fruit Persea americana. (eds.): Amsterdam, Netherland: Elsevier, 37–48.
15. Koroch, A. R., Rodolfo Juliani, H., & Zygadlo, J. A. (2007): Bioactivity of essential oils and their components. Flavours and fragrances, 87-115.
16. Landero-Valenzuela N., Lara-Viveros F.M., Andrade-Hoyos P., Aguilar-Pérez L.A., Aguado, G.J. (2016): Alternativas para el control de Colletotrichum spp. Revista Mexicana de Ciencias Agrícolas, 7: 1189- 1198.
17. Macias F. A., Castellano D., Oliva R. M., Cross P., Torres A. (1997): Potential use of allelopathic agents as natural agrochemicals. (eds.): Elsevier: 37–48.
18. Marin O. J., M.J Barrera., J Robles., J. (2008): Evaluación de extractos vegetales para el control de Mycosphaerella fijiensis en platano en Tierralta- Córdoba. Temas Agrarios, 1: 25-31.
19. Nazarro F., Fratianni F., De Martino L., Coppola R., De Feo V. (2013): Effect of Essential Oils on Pathogenic Bacteria. Pharmaceuticals, 6: 1451-1474.
20. Torres-González C., Casas M., Díaz Ortiz J. E. (2013): Manejo de Ralstonia Solanacearum raza 2 a través de productos químicos y biológicos. Iteckne, 10: 217-223.
21. Ramírez J. G., Gonzalo J., Peterson A. (2018): Potential geography and productivity of “Hass” avocado crops in Colombia estimated by ecological niche modeling. Scientia Horticulturae. 237: 287–295.
22. Rao G., Singh M., OSingh H. (1992): Fungitoxic evaluation of essential oils extracted from higher plants against some sugarcane pathogens in vitro. Tropic Sci, 32: 377-382.
23. Rimá de Oliveira K. Á., Ramos Berger L. R., Amorim de Araújo S., Saraiva Câmara M. P., & Leite de Souza, E. (2017): Synergistic mixtures of chitosan and Mentha piperita L. essential oil to inhibit Colletotrichum species and anthracnose development in mango cultivar Tommy Atkins. Food Microbiology, 66: 96-103.
24. Rondón O., Sanabría de Albarracín N., Rondón, A. (2008): Respuesta In vitro a la acción de fungicidas para el control de antracnosis, Colletotrichum gloeosporioides Penz, en frutos de mango. Agronomía Tropical, 56: 219-235.
25. Cifuentes-Arenas J. C., Beattie G. A. C., Peña L., Lopes, S. A. (2019): Murraya paniculata and Swinglea glutinosa as short-term transient hosts of ‘Candidatus Liberibacter asiaticus’ and implications for the spread of huanglongbing. Phytopathology, 109(12): 2064-2073.
26. Pérez-Cordero A., Chamorro-Anaya L., Mercado-Gómez J. D. (2021): Inhibición de Colletotrichum gloeosporioides en cultivos de ñame en el Caribe colombiano usando aceites esenciales de Curcuma longa y Zingiber officinale. Ciencia en Desarrollo, 12: 1-12.