HISTOPATHOLOGICAL, ANTIOXIDANTS, BIOCHEMICAL EXAMINATION AND HEPATOPROTECTIVE EFFECTS OF CANNABIS SATIVA AGAINST CCL4 INDUCED TOXICITY IN RATS

Main Article Content

Munib Ashfaq
Hafiz Muhammad Arsalan
Sheraz Yasin
Amina Arif
Hina Mumtaz
Kurmanbek Uulu Beksultan

Keywords

CCl4, SOD, CAT, GSH, MDA, Cannabis Sativa, Hepatoprotectivity

Abstract

Cannabis's historical shift from natural medicine to illegal substance and back to therapeutic product upon legalization open a new age application as anti-inflammatory during COVID-19. This motivated to explore the anti-inflammatory, biochemical and hepatoprotective profiling of cannabis sativa. Carbon tetra chloride is a well-known xenobiotic hepatotoxic chemical that attacks cytochromes P450 enzymes of the liver. That produces free radicals, which starts lipid peroxidation, leading to loss of Ca2+ and causing liver injury. To determine the hepato-toxic effect of Carbon Tetra Chloride and Hepato-protective effects of Cannabis Sativa and their correlation between CCl4, Cannabis Sativa and antioxidant activity in albino rats. Ethanolic extract of C. Sativa (50 mg/kg + CCl4) and (100 mg/kg + CCl4) was used thrice a week for five weeks. Hepatotoxicity was induced in albino rats by using CCl4. Normal Group, CCl4 induced Hepato-toxicity (0.5ml / kg), Ethanolic extract of C. Sativa treated (50mg / kg + CCl4) and Ethanolic extract of C. sativa (100mg/kg + CCl4). 5.0 ml Blood samples of all groups (Group A, B, C and D) were taken in gel clotted vial and centrifuged at 4000 rpm for 10 minutes and serum was separated. Serum samples were further processed for the estimation of Reduce Glutathione (GSH), Catalyze (CAT), Superoxide Dismutase (SOD), Malondialdehyde (MDA) Estimation of Nitric oxide (NO), Estimation of micronutrients (Vitamin A, Vitamin C and Vitamin E), and Electrolytes concentration by a flame photometer (Na+ and K+), estimation of AGE’s and AOPP, Liver Function Test (LFTs), Lipid Profile, Serum Albumin and Histopathological examination. Induction of CCl4 significantly increased Malondialdehyde (MDA) Catalyze (CAT) Nitric Oxide (NO) Advance Glycation end product (AGE’s) and Vitamin –A (VIT-A) while decreasing the Super Oxide Dismutase, LFTs and RFTs in serum. Remedy of C. sativa 50mg/kg and 100mg/kg body weight noticeably defended the rats from CCl4 administered liver damage. Overall 50mg/kg + CCl4 dose is more valid and promising to cure the hepatic damage induced by CCl4. The findings show that C. sativa has the capacity to protect against CCl4-mediated liver damage. These activities may be related to the synergistic impact of the identified substances and probable interactions with the hepatic system. It can be recognized as a food appendage against liver reperfusion.


 

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