EVALUATION OF AZADIRACHTA INDICA LEAVES EXTRACT FOR ANTI-ALLERGIC POTENTIAL

Main Article Content

A. Helen Sonia
Pawar Kavita Yogesh
Sonali V Uppalwar
Romila Antony
B. Sathya
Payal Rani
Rajiv Bharti
Biresh Kumar Sarkar
Dhammshila L Devhare

Keywords

Azadirachta indica, allergy, ethanolic, nimbin, histamine

Abstract

This study investigates the antiallergic potential of Azadirachta indica leaves extract as a natural remedy for allergic conditions. Azadirachta indica, commonly known as Neem, has a history of medicinal use in traditional medicine systems. The ethanolic extract was prepared from the leaves and evaluated in allergen-induced allergic reactions using animal models and in-vitro process. The extract demonstrated significant antiallergic effects, reducing histamine release, suppressing pro-inflammatory cytokines, and enhancing immunomodulatory activity. These findings support the traditional use of Azadirachta indica and its potential as an antiallergic agent. The presence of bioactive compounds like nimbin, nimbidin, and azadirachtin may contribute to its antiallergic properties. Further research is needed to elucidate the underlying mechanisms and evaluate the extract's safety and efficacy in human subjects. If proven effective, Azadirachta indica leaves extract could offer an affordable and accessible alternative for managing allergic conditions.

Abstract 289 | pdf Downloads 0 pdf Downloads 134

References

1. Sharma M, Sharma AK, Kumar A. Azadirachta indica: A herbal panacea in dermatology. Int J Pharm Sci Res. 2018; 9(7):2617-28.
2. Gupta SC, Prasad S, Tyagi AK, et al. Neem (Azadirachta indica): An Indian traditional panacea with modern molecular basis. Phytomedicine. 2017; 34:14-20.
3. Siddiqui BS, Afshan F, Gulzar T, Sultana R. A comprehensive review on biological activities of neem (Azadirachta indica). Life Sci. 2021; 281:119808.
4. Tiwari R, Verma AK, Srivastava SK, et al. Therapeutic uses of Azadirachta indica (Neem) leaves: A review. J Chem Pharm Res. 2011; 3(1):10-14.
5. Rehman MU, Ali M, Khan R, et al. Antihistaminic and anticholinergic effects of nimbolide: A new antiallergic agent from Azadirachta indica. Nat Prod Res. 2014; 28(18):1548-51.
6. Biswas K, Chattopadhyay I, Banerjee RK, Bandyopadhyay U. Biological activities and medicinal properties of neem (Azadirachta indica). Curr Sci. 2002; 82(11):1336-45.
7. Singh D, Singh G, Kashyap A. Pharmacological potential of Azadirachta indica: A review. J Pharmacogn Phytochem. 2018; 7(3):1741-49.
8. Dey D, De R, Ghosh AK, et al. Evaluation of anti-allergic property of methanolic extract of Azadirachta indica leaves in rodents. Indian J Pharmacol. 2012; 44(3):357-60.
9. Gupta R, Sharma AK, Sharma MC, et al. Therapeutic efficacy of Azadirachta indica leaves in experimental models of allergic rhinitis. Int J Pharmacol. 2019; 15(2):172-79.
10. Singh A, Mehta A, Tripathi P, et al. Evaluation of antiallergic potential of Azadirachta indica in a murine model of asthma. J Tradit Complement Med. 2020; 10(1):59-65.
11. Patel, J. R., Tripathi, P., Sharma, V., Chauhan, N. S., & Dixit, V. K. (2010). Evaluation of antiallergic activity of Phyllanthus emblica (amla) fruits in isolated goat tracheal chain preparation. Pharmaceutical Biology, 48(4), 461-468. doi: 10.3109/13880200903190982
12. Waghmare, A. B., Chavan, M. J., Bhaskar, V. H., & Gaviraj, E. N. (2019). Antiallergic potential of Aegle marmelos in mast cell-mediated allergy model. International Immuno pharmacology, 75. doi: 10.1016/j.intimp.2019.105819
13. Muma, N. A., & Mathis, D. A. (1988). Clonidine-induced catalepsy: time course, drug interactions, and dopamine receptor involvement. Journal of Pharmacology and Experimental Therapeutics, 246(1), 47-54.
14. Florijn, W. J., van Luijtelaar, E. L., & van den Hoogen, R. H. (1990). Influence of clonidine and diazepam on haloperidol-induced catalepsy in rats. European Journal of Pharmacology, 182(2), 361-365. doi: 10.1016/0014-2999(90)90352-9
15. Sharma, V., Tripathi, P., Patel, J. R., Chauhan, N. S., & Dixit, V. K. (2011). Evaluation of antiallergic activity of ethanolic extract of Azadirachta indica leaves in animal models. Pharmaceutical Biology, 49(4), 413-419. doi: 10.3109/13880209.2010.529064
16. Mahesh, A., Jeyachandran, R., Cindrella, L., Thangadurai, D., Veerapur, V. P., & Muralidhara Rao, D. (2011). Nimbidin suppresses functions of dendritic cells through down-regulation of pro-inflammatory cytokines and antigen uptake, and impairment of T cell stimulation. European Journal of Pharmacology, 657(1-3), 69-79. doi: [DOI]
17. Parveen, S., Alam, P., Sahoo, H. B., & Rizvi, M. M. A. (2012). Immunomodulatory effect of ethanolic extract of Azadirachta indica leaves on mast cells in vitro and in vivo. Asian Pacific Journal of Tropical Biomedicine, 2(1), S37-S40. doi: 10.1016/S2221-1691(12)60132-7
18. Ali, M. B., Alam, P., Alam, N., & Siddiqui, M. S. (2013). Comparative immunomodulatory activities of ethanolic extracts of Azadirachta indica, Allium sativum, and Datura stramonium in animals. Indian Journal of Pharmacology, 45(1), 68-71. doi: 10.4103/0253-7613.106435
19. Saeed, N., Khan, M. R., Shabbir, M., & Muhammad, N. (2016). Urtica dioica (Stinging Nettle): A review of its chemical, pharmacological and therapeutic potential. Journal of Ethnopharmacology, 189, 135-146. doi: 10.1016/j.jep.2016.05.042
20. Golandaz G, Pal A, Vaibhav Uplanchiwar, Rupesh Gautam. A Butea Monosperma flower partially reduces high fat diet induced obesity in experimental rats. Obesity Medicine, 17(2020) 100179. doi: https://doi.org/10.1016/j.obmed.2019.100179.
21. Vaibhav Uplanchiwar, M.K. Gupta, Rupesh K. Gautam. Memory enhancing effect of various polar and nonpolar extracts of Plumbago Zeylanica Linn. Roots. International Journal of Green Pharmacy, Jan-June 2018 (Suppl).12 (1).
22. Sushil Raut, Vaibhav Uplanchiwar, Avinash Gahane, Santosh Bhadoriya, Shrishail Patil, Sunil K Jain. Development, characterization and investigation of anti-inflammatory potential of valdecoxib topical gels. Journal of Scientific & Industrial Research Vol. 71, April 2012, pp. 273-278
23. Uplanchiwar, Vaibhav P., Sushil Yadaorao Raut, and Lalchand D. Devhare. "Pharmacological assessment of antiulcer activity of gloriosa superba linn tubers in experimentally induced gastric ulcers." Journal of medical pharmaceutical and allied science 10.3 (2021): 2852-2856.
24. Anuj Modi, Vimal kumar Jain, Prateek Jain, Sunil Jain, Vaibhav Uplanchiwar. Evaluation of antioxidant activity of flavonoids and phenolic content Luffa Echinata Roxb. Fruits and Nyctanthus Arbor-Tristis leaves. International Journal of Phytopharmacy, 1, 2011.
25. Devhare, L. D., Ghugare, A. P., & Hatwar, B. P. (2015). Method development for determination of water content from various materials by spectrophotometry and it’s validation. International journal of drug delivery, 7(4), 233-240.
26. Devhare, L. D., & Kore, P. K. (2016). A recent review on bioavailability and solubility enhancement of poorly soluble drugs by physical and chemical modifications. Research chronicle in health sciences, 2(5), 299-308.
27. Tonde, T. U., Kasliwal, R. H., & Devhare, L. D. (2016). Quantitative Estimation of Bacoside A in Polyherbal Memory Enhancer Syrup for Memory Boosting Activity Using HPTLC Method. Research Chronicle in Health Sciences, 2(6), 315-320.
28. Ghugare, A. P., Devhare, L. D., & Hatwar, B. P. (2016) Development and validation of analytical methods for the simultaneous estimation of Nimorazole and Ofloxacin in tablet dosage form. 8(3), 96-98.
29. Salpe, H. G., Devhare, L. D., Ghugare, A. P., & Singh, N. (2016). Formulation and evaluation of hpmc coated diltiazem hcl tablet and its comparison with other marketed preparation. Research chronicle in health sciences. 3(1), 11-17
30. Makhani, A. A., & Devhare, L. D. (2017). Development and validation of vierordt's spectrophotometric method for simultaneous estimation of Drotaverine and Nimesulide combination. Research chronicle in health sciences, 3(2), 22-28.
31. Makhani, A. A., & Devhare, L. D. (2017). Development and Validation of Analytical Methods for Drotaverine and Nimesulide Combination. Research Chronicle in Health Sciences, 3(3), 40-44.
32. Katole, G., & Devhare, L. D. (2020). Recent insight into some emerging natural resources with remarkable hepato protective potentials. International journal of pharmaceutical science and research, 5(1), 41-47.
33. Uplanchiwar, V. P., Raut, S. Y., & Devhare, L. D. (2021). Pharmacological assessment of antiulcer activity of gloriosa superba linn tubers in experimentally induced gastric ulcers. Journal of medical pharmaceutical and allied science, 10(3), 2852-2856.
34. Devhare, L. D., & Gokhale, N. (2021). Acid neutralizing capacity and antimicrobial potential of selected solvent extract from various indigenous plants. Journal of Advanced Scientific Research, 12(04), 175-179.
35. Devhare, L. D., & Gokhale, N. (2022). Antioxidant and Antiulcer property of different solvent extracts of Cassia tora Linn. Research Journal of Pharmacy and Technology, 15(3), 1109-1113.
36. Devhare, L. D., & Gokhale, N. (2023). In silico anti-ulcerative activity evaluation of some bioactive compound from Cassia tora and Butea monosperma through moleculer docking approach. International journal of pharmaceutical sciences and research, 14(2), 1000-1008.
37. Devhare, L. D., & Gokhale, N. (2023). A brief review on: phytochemical and antiulcer properties of plants (fabaceae family) used by tribal people of gadchiroli maharashtra. International journal of pharmaceutical sciences and research, 14(4), 1572-1593.
38. Nikam N, R., Vaishnavi, A., & Devhare, L. D. (2023). Parenteral drug delivery approach: an overview. Journal of xidian university, 17(1), 386-400.
39. Shende, S. M., Bhandare, P., & Devhare, L. D. (2023). In-vitro: micropropagation of mint and investigate the antibacterial activity of mint extract. Eur. Chem. Bull, 12(5), 780-784.
40. Bodhankar, S. S., Devhare, L. D., Meshram, A. S., Moharkar, D. W., & Badwaik, C. B. (2023). Formulation and in vitro evaluation of dental gel containing ethanglic extract of Mimosa pudica. European Chemical Bulletin, 12(5), 1293-1299.
41. Devhare, L. D., Bodhankar, S. S., Warambhe, P., Uppalwar, S. V., Uchibagle, S., & Shende, S. M. (2023). Important role of food and nutritional security during Covid-19: A survey. European Chemical Bulletin. 12(5), 1363-1374.
42. Pathak, N. R., Devhare, L. D., Sawarkar, K. R., Dubey, M., Trivedi, V., Thakre, A. R., & Thakare, V. M. (2023). Aclinial reveiew on pharmacological evaluation of Thiazolidine and Isatin in the new millenium as magic moieties. European Chemical Bulletin. 12(5), 3410-3417.
43. Singh, S., Minj, K. H., Devhare, L. D., Uppalwar, S. V., Anand, S., Suman, A., & Devhare, D. L. (2023). An update on morphology, mechanism, lethality, and management of dhatura poisoning. European Chemical Bulletin. 12(5), 3418-3426.
44. Suruse, P. B., Jadhav, B. A., Barde, L. G., Devhare, L. D., Singh, S., Minj, K. H., & Suman, A. (2023). Exploring the potential of Aerva Lanata extract in a herbal ointment for fungal infection treatment. Journal of Survey in Fisheries Sciences. 10(1), 1922-1932.
45. Shende, S. M., Meshram, B., Karemore, H., & Devhare, L. D. (2023). Development And Characterization of Glycerogelatin Suppositories For Enhanced Efficacy. European Journal of Pharmaceutical and Medical Research. 10(6), 522-528.
46. Thakare, V. M., Umare, S. A., & Devhare, L. D. (2023). Separation and purification of carboxymethyl cellulose from Spinacia Oleracea for use in pharmaceutical dosage form. European Chemical Bulletin. 12(5), 4062-4080.
47. Suruse, P. B., Deshmukh, A. P., Barde, L. G., Devhare, L. D., Maurya, V. K., Deva, V., & Priya, N. S. (2023). Rimegepant embedded fast dissolving films: A novel approach for enhanced migraine relief. Journal of Survey in Fisheries Sciences, 10(1) 2071-2084.
48. Prasad, M., Suman, A., Srivastava, S., Khosla, G., Deshmukh, A., Devhare, L. D., & Meshram, S. S. Butea monosperma stem bark extract partially reverses high fat diet-induced obesity in rats. European Chemical Bulletin. 12(5), 4267 – 4273.
49. Shukla, M., Tiware, S. A., Desai, S. R., Kumbhar, S. T., Khan, M. S., Mavai, Y., & Devhare, L. D. (2023). Pharmacological Evaluation of Gloriosa Superba Linn Flower Extract For Antiulcer Activity. Journal of Survey in Fisheries Sciences. 10(2) 463-470.
50. Polireddy, P., Malviya, V., & Devhare, L. D. (2023). Assessment of Hepatoprotective Potential of Ecbolium Linneanum Extract on Experimental Animals. Journal of Coastal Life Medicine. 2(11) 884-890
51. Devhare, L. D., Hiradeve, S. M., & Bobade, T. (2017). Method Development & Validation For Determination of Water Content. LAP LAMBERT Academic Publishing.
52. Shukla, M., Tiware, S. A., Desai, S. R., Kumbhar, S. T., Khan, M. S., Mavai, Y., & Devhare, L. D. (2023). Pharmacological Evaluation of Gloriosa Superba Linn Flower Extract For Antiulcer Activity. Journal of Survey in Fisheries Sciences, 10(2) 463-470.
53. Polireddy, P., Malviya, V., Arora, S., Singh, M., Pooja Tanaji, G., Devhare, L. D., & Dharmamoorthy, G. (2023). Assessment of Hepatoprotective Potential of Ecbolium Linneanum Extract on Experimental Animals. Journal of Coastal Life Medicine, 11(2) 884-890.
54. Singh, M., Malik, A., Devhare, D. L., Ruikar, D. B., Krishnan, K., Kumar, D. V., & Devnani, D. (2023). Comparative Case Study on Tuberculosis Patients Between Rural And Urban Areas. Journal of Survey in Fisheries Sciences, 10(2) 622-632.
55. Devhare, L. D., Kumbhar, S. T., Chitrapu, P., Kundral, S., & Borkar, A. A. (2023). In-Silico Molecular Docking Study of Substituted Imidazo 1, 3, 4 Thiadiazole Derivatives: Synthesis, Characterization, and Investigation of their Anti-Cancer Activity. Journal of Coastal Life Medicine, 11(2) 1237-1245.
56. Thakre, S. M., Kumar, D. V., Ahuja, A., Hamid, N., Thakre, A. R., Khan, M. S., & Devhare, D. L. (2023). Exploring the Influence of an Antifungal Medication on Patients Receiving Oral Hypoglycemic Therapy: Investigating the Interplay Between Medications. Journal of Coastal Life Medicine, 11(2) 1255-1262.
57. Devhare, L. D., Katole, G. (2018) Diluent and granulation study on Metformin Hydrochoride. LAP LAMBERT Academic Publishing.