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Vishal Ramesh Rasve
Anup Kumar Chakraborty
Sachin Kumar Jain
Sudha Vengurlekar


antidiabetic, α-glucosidase, streptozotocin, SMEDDS, Clematis Triloba, α-Amylase


Objectives: The present study aimed to evaluate and compare the antidiabetic activity of ethanolic leaves extract of Clematis triloba and their SMEDDS formulation with Glibenclamide (GL) by α-amylase, α-glucosidase enzyme inhibitory assay and streptozotocin induced diabetic rats.

Methods: Using the 3,5-dinitrosalicylic acid method and 4-Nitrophenyl--D-glucopyranoside, respectively, the α-amylase inhibition and α-glucosidase enzyme inhibitory activities of Clematis Triloba extracts were assessed. In several animal models, including hypoglycemic, oral glucose-loaded, and streptozotocin-induced diabetic rat models, the extract and SMEDDS formulation ability to reduce levels of blood glucose was investigated.

Results: The enzymes α-amylase and α-glucosidase were significantly inhibited by the clematis triloba ethanolic extract in a dose-dependent manner. When compared to an ethanolic extract, the SMEDDS formulation of clematis triloba showed greater antidiabetic efficacy.

Conclusion: The current research suggests that clematis triloba's ethanolic leaf extracts have in vitro and in-vivo anti-diabetic action. This study showed that these herbs have great therapeutic efficacy in treating diabetes mellitus and its accompanying problems, hence proving their traditional applications in Indian traditional medicine.

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