Age Estimation Using Exfoliative Cytology and CBCT – A Comparative Study

Main Article Content

Kalaiselvi Santhosh
Anu Sushanth A
Sasirekha. B
Ishwariya Krishnan
Niroshini Rajaram

Keywords

Age estimate, exfoliation cytology, cone beam computed tomography, and pulp-tooth area ratio

Abstract

Introduction: One of the most crucial steps in establishing someone's identification is making an educated guess about their age. One method is exfoliate cytology (EC), which involves painlessly and easily collecting unharmed cells from the oral cavity for microscopic examination. Pulp-to-tooth area ratio (PTR) measurements utilising cone beam computer tomography (CBCT), which is a 3D image of teeth in living humans, are another innovative nondestructive way of age estimate
The motivation behind this study is to decide a singular's age by estimating the pulp-to-tooth area ratio (PTR) of maxillary canines through the utilization of three-layered cone beam computed tomography (CBCT),(CBCT),and by analysing the average cell size of buccal smears through the use of image morphometric software in exfoliative cytology.
From 100 patients who seemed to be in good health, buccal swabs were obtained. The pulp and tooth regions were marked using the Kodak 9600 3D digital imaging device on the CBCT images of the mandibular canines.
Results: Using a paired t test, we found that there was no significant difference between the predicted age and the actual age using cell size and the pulp-tooth area ratio (P > 0.05). In the current investigation, CBCT and EC both yielded similarly accurate ages based on the pulp-tooth area ratio.

Abstract 160 | PDF Downloads 133

References

1. Afify MM, Zayet MK, Mahmoud NF, Ragab AR. Age estimation from pulp/tooth area ratio in three mandibular teeth by panoramic radiographs: Study of an Egyptian sample. J Forensic Res 2014;5:1‑5.
2. Anuradha A, Sivapathasundharam B. Image analysis of normal exfoliated gingival cells. Indian J Dent Res 2007;18:63‑6.
3. Azevedo Ade C, Alves NZ, Michel‑Crosato E, Rocha M, Cameriere R, Biazevic MG. Dental age estimation in a Brazilian adult population using Cameriere’s method. Braz Oral Res 2015;29. pii: S1806‑83242015000100215.
4. Babshet M, Acharya AB, Naikmasur VG. Age estimation in Indians from pulp/tooth area ratio of mandibular c anines. Forensic Sci Int 2010;197:125‑34.
5. Cameriere R, Ferrante L, Belcastro MG, Bonfiglioli B, Rastelli E, Cingolani M. Age estimation by pulp/tooth ratio in canines by peri‑apical x‑rays. J Forensic Sci 2007;52:166‑70.
6. Cameriere R, Ferrante L, Cingolani M. Precision and reliability of pulp/tooth area ratio (RA) of second molar as indicator of adult age. J Forensic Sci 2004;49:1319‑23.
7. Joseph CC, Reddy BH, Cherian NM, Kannan SK, George G, Jose S. Intraoral digital radiography for adult age estimation: A reliable technique. J Indian Acad Oral Med Radiol 2013;25:287‑90.
8. Juneja M, Devi YB, Rakesh N, Juneja S. Age estimation using pulp/ tooth area ratio in maxillary canines‑A digital image analysis. J Forensic Dent Sci 2014;6:160‑5.
9. Kumaresan GD, Jagannathan N. Exfoliative cytology – A predictive diagnostic tool. Int J Pharm Sci 2014;6:1‑3.
10. Ohtani S. Estimation of age from the teeth of unidentified corpses using the amino acid racemization method with reference to actual cases. Am J Forensic Med Pathol 1995;16:238‑42.
11. Papanicolaou GN. A new procedure for staining vaginal smears. Science 1942;95:438‑9.
12. Patel PV, Kumar S, Kumar V, Vidya G. Quantitative cytomorphometric analysis of exfoliated normal gingival cells. J Cytol 2011;28:66‑72.
13. Reddy VS, Kumar GS, Vezhavendhan N, Priya S. Cytomorphometric analysis of normal exfoliative cells from buccal mucosa in different age groups. Int J Clin Dent Sci 2011;2:53‑6.
14. Shetty DC, Wadhwan V, Khanna KS, Jain A, Gupta A. Exfoliative cytology: A possible tool in age estimation in forensic odontology. J Forensic Dent Sci 2015;7:63‑6.
15. Solheim T, Vonen A. Dental age estimation, quality assurance and age estimation of asylum seekers in Norway. Forensic Sci Int 2006;159 Suppl 1:S56‑60.
16. Star H, Thevissen P, Jacobs R, Fieuws S, Solheim T, Willems G. Human dental age estimation by calculation of pulp‑tooth volume ratios yielded on clinically acquired cone beam computed tomography images of monoradicular teeth. J Forensic Sci 2011;56 Suppl 1:S77‑82.
17. Wood RE. Forensic aspects of maxillofacial radiology. Forensic Sci Int 2006;159 Suppl 1:S4 ‑55.
18. Muhammad Khan Asifa , Phrabhakaran Nambiara,b, , Shani Ann Manic , Norliza Binti Ibrahima , Iqra Muhammad Khanc . Dental age estimation in Malaysian adults based on volumetric analysis of pulp/tooth ratio using CBCT data Legal Medicine 36 (2019) 50–58
19. Acharya AB, Vimi S. Effectiveness of Bang and Ramm’s formulae in age assessment of Indians from dentin translucency length. Int J Legal Med 2009;123:483-8