Molecular Study of the Toxic Shock Syndrome Gene Tsst-1 in Staphylococcus Aureus Isolated from Different Female Cases

Main Article Content

Afifah Mohammed Wali
Rana S. Al-Taweel

Keywords

Molecular, Isolated, Toxic, Syndrome

Abstract

This study aimed at molecular investigation of the toxicity gene for toxic shock syndromeTsst-1, which is encoded by the T gene, produced by Staphylococcus aureus and isolated from multiple female cases. Accordingly, approximately 180 samples were collected from different female cases from inpatients and patients attending Imam Al-Sadiq Hospital, Al-Qasim General Hospital, and Al-Hashimiyya Hospital in Babylon Governorate.
Biochemical tests were carried out for the samples taken, where they were cultured on blood agar medium, and it was noted that there were significant differences at a level of significance of 5%, and the culture results were 120 (66.7%) positive bacterial isolates for Gram stain, compared to no recording of 60 (33.3%) samples of bacterial growth on blood agar media. Staphylococcus aureus 45 isolates (37.5%) were cultured on mannitol-saline medium and the diagnosis was confirmed byAPI staph. and the phaetek system (Vitek 2), where the results showed that there were no significant differences at the level of significance of 5% for the isolates grown on manthol saline medium. The drug sensitivity of the Staphylococcus aureus isolates towards antibiotics was tested by using the disc spread method on Acar Mueller-Hinton medium and according to the Karby-Bauer method. There are significant differences at the level of significance of 5%, and some virulence factors were investigated outwardly, as significant differences were found at the level of significance of 5%. Among the virulence factors were the formation of the biofilm, the percentage of which was 89%, the production of hemolytic enzyme by 100%, and the production of the enzyme coagulase (plasma coagulase). ) by 100% 

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References

1. Jaber, Rehab Dawas. (2022). Molecular characterization of some virulence factors and aminoglycoside-modifying enzymes in bacteriaStaphylococcus aureusIsolated from different clinical cases. Master's thesis. College of Education, Al-Qadisiyah University, Iraq.
2. Senok AC, Verstraelen H, Temmerman M, Botta G A. (2009). Probiotics for the treatment of bacterial vaginosis. Cochrane Database Syst Rev: CD006289.
3. Zainulabdeen, SM and Dakl, AAA (2021). Pathogenicity and virulence factors in Staphylococcus aureus. Malaysian Journal of Pharmaceutical Sciences, 8(1), 109-119.
4. Mahfouz, AA; Al-Azraqi, TA; Abbag, F.I. ; Al-Gamal, MN & Seef , S.t. (2010) Nosocomial infections in a neonatal intensive care unit in
southwestern Saudi Arabia. East Mediterrer Health J 16:40-44.
5. Krakauer, T. (2019). Staphylococcal Superantigens: Pyrogenic Toxins Induce toxic shock. Toxins, 11(3), 178.
6. Plata, K., Rosato, AE, & Wegrzyn, G. (2009). Staphylococcus aureus as an infectious agent: overview of the biochemistry and molecular genetics of its pathogenicity. ActaBiochimica Polonica, 56(4), 597
7. Hakimi, AR, Mohammadzadeh, A., Mahmoodi, P., and Alikhani, M. Y. (2018). Detection of Toxic Shock Syndrome Toxin (tsst) Gene Among Staphylococcus aureus Isolated from Patients and Healthy Carriers.
8. Lu, M., Fu, M., Zhang, Y., Shen, T., Xie, H. and Liu, D. (2022). Septicaemia with deep venous thrombosis and necrotising pneumonia caused by an acute community-acquired methicillin-resistant Staphylococcus aureus in an infant with a three-year follow-up: a case report. BMC Infectious Diseases, 22(1), 1-6
9. Sapugahawatte, DN, Li, C., Yeoh, YK, Dharmaratne, P., Zhu, C., & Ip, M. (2020). Swine methicillin-resistant Staphylococcus aureus carrying toxic-shock syndrome toxin gene in Hong Kong, China. Emerg Microbes Infection, 9(1), 1534-1536
10. Hassan, A.; Usman, J.; Kaleem, Fatima.; Omar, M.; Khalid, A. and Iqbal, M. (2011). Evaluation of different detection methods of biofilm formation in the clinical isolates. braz. J. Infect. dis.,15(4), 305-311
11. Chart, H., Jenkins, C., Smith, HR, Hedges, D. and Rowe, B. (1998). Haemolysin production by strains of verocytotoxin-producing Escherichia coli. Microbiology, 144(1), 103-107
12. MacFaddin, JF (2000). Biochemical tests for medical identification bacteria. 3rd ed. the Williams and Wilkins. London.
13. Sambrook, J.; Friegan, E.; & Miniatis, T. (1989). Molecular cloning laboratory manual, Cold Spring Harbor Laboratory. New York
14. Al-Douri, AYM (2011). Diagnostic and genetic study of the bacteria causing urinary tract infections in patients in tikrit city. ph. D.thesis. college of education. University of Tikrit
15. Al-Omari, AW; Mohamad AM and Raoof WM (2013). Detection of Biofilm Formation in some Pathogenic Bacteria Using Tube and Congo Red Agar Methods. J. of Rafidain science., 24(6): 55-65
16. Ratajczak, M., Kaminska, D., Dlugaszewska, J. and Gajecka, M. (2021). Antibiotic resistance, biofilm formation, and presence of genes encoding virulence factors in strains isolated from the pharmaceutical production environment. Pathogens, 10(2), 130-143