Molecular Study of the Toxic Shock Syndrome Gene Tsst-1 in Staphylococcus Aureus Isolated from Different Female Cases

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Afifah Mohammed Wali
Rana S. Al-Taweel


Molecular, Isolated, Toxic, Syndrome


This study aimed at molecular investigation of the toxicity gene for toxic shock syndromeTsst-1, which is encoded by the T gene, produced by Staphylococcus aureus and isolated from multiple female cases. Accordingly, approximately 180 samples were collected from different female cases from inpatients and patients attending Imam Al-Sadiq Hospital, Al-Qasim General Hospital, and Al-Hashimiyya Hospital in Babylon Governorate.
Biochemical tests were carried out for the samples taken, where they were cultured on blood agar medium, and it was noted that there were significant differences at a level of significance of 5%, and the culture results were 120 (66.7%) positive bacterial isolates for Gram stain, compared to no recording of 60 (33.3%) samples of bacterial growth on blood agar media. Staphylococcus aureus 45 isolates (37.5%) were cultured on mannitol-saline medium and the diagnosis was confirmed byAPI staph. and the phaetek system (Vitek 2), where the results showed that there were no significant differences at the level of significance of 5% for the isolates grown on manthol saline medium. The drug sensitivity of the Staphylococcus aureus isolates towards antibiotics was tested by using the disc spread method on Acar Mueller-Hinton medium and according to the Karby-Bauer method. There are significant differences at the level of significance of 5%, and some virulence factors were investigated outwardly, as significant differences were found at the level of significance of 5%. Among the virulence factors were the formation of the biofilm, the percentage of which was 89%, the production of hemolytic enzyme by 100%, and the production of the enzyme coagulase (plasma coagulase). ) by 100% 

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