Real time and rapid detection of NDM 1 gene in beta lactam resistant bacteria using electrochemical assay based aptagene nanoreceptors

Hisham F. Mohammad; Hanan S. Makii; Abbas A. AL Hasani; Muna M. AL Hammadani

doi:10.47750/jptcp.2023.30.13.036

Hisham F. Mohammad

College of Science, University of Basrah, Iraq

Hanan S. Makii

Basrah Children Specialty Hospital, Basrah Province, Iraq

Abbas A. AL Hasani

College of Medicine, University of Basrah, Basra, Iraq

Muna M. AL Hammadani

Basrah Children Specialty Hospital, Basrah Province, Iraq

Keywords

NDM-1 gene, aptamer binding, PCR amplification, aptasensor, single strand DNA

Abstract

Background: New Delhi Metallo b lactamase (NDM 1) is an enzyme that makes bacteria resistant to a broad range of beta lactam antibiotics
Methods: In this study, the target gene was NDM-1 of beta lactam resistant bacteria which isolated on Mueller Hinton Agar (MHA) and identified via DNA extraction and PCR amplification then compared with the electrochemical assay based aptamer nanoreceptors.
Results: The results were encouraged and indicate that specific DNA aptasensor identified signal based the aptamer NDM-1 gene binding and exhibited affinity for the target DNA single strand, resulting in the direct detection of the NDM-1 in complex clinical bacteria samples in compare with PCR amplification.
Conclusions: This novel electrochemical gene sensor method allows for the cost effective, rapid, specific, and more sensitive detection of the NDM-1.

Abstract 122 | PDF Downloads 94

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Published

Jun 4, 2023

DOI https://doi.org/10.47750/jptcp.2023.30.13.036

How to Cite

Hisham F. Mohammad, Hanan S. Makii, Abbas A. AL Hasani, & Muna M. AL Hammadani. (2023). Real time and rapid detection of NDM 1 gene in beta lactam resistant bacteria using electrochemical assay based aptagene nanoreceptors. Journal of Population Therapeutics and Clinical Pharmacology, 30(13), 354–361. https://doi.org/10.47750/jptcp.2023.30.13.036