Detection of Escherichia coli O157:H7 strain in diabetic foot infection patients in Basrah, Iraq

Main Article Content

Zainab Mohammed Hussein
Lina Abbas Naser
Abdulhussein Khudair Marzoq

Keywords

shigatoxin, Enterohemorgic E. coli ,Hi-Chrome EC O157:H7, ECVt1

Abstract

Diabetic foot ulcers (DFUs) are defined by the typical triad of neuropathy, ischemia, and infection. The shigatoxin, Enterohemorgic E. coli, is considered one of the most dangerous types of toxins caused by a strain of E. coli O157:H7 for diabetic foot patients, which is transmitted through contamination with it, causing uremic hemolytic syndrome and renal failure for those with impaired kidney function.
To detect virulence factors, medium-high chrom was used after addition of supplements; serotyping was used; and it was revealed by the molecular method using the specific gene ECVt1.
This study showed a strain on Hi-Chrome medium, and the colonies showed a dark purple color after the addition of its supplement, Novoboicin, and Calcium Tellurite. 16(38.09%) As for serotyping, the study showed a strain after purification on Maconkey sorbitol medium 7 (16.7%). The study revealed a gene, ECVt1, of this strain, 9 (21.43%), which has a size of 637 bp.
Do not use antibiotics for this strain, as they will increase its toxicity and may lead to death.
The chromogenic substrate is broken down by E. coli O157:H7 and produces a dark purple to magenta-colored moiety. Samples were cultured on MacConkey sorbitol agar for the purpose of testing them serologically to ensure the appearance of E. coli. O157:H7, where the colonies are not sorbitol fermented Genetic detection confirmed the existence of the gene ECVt1 belonging to enterohememorragic E. coli, the main cause of this strain O157:H7

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