Evaluation of the antioxidant efficacy of Terpenes isolated from the Zingiber officinale Roscoe in treating experimentally alloxan-induced diabetes in Male Albino Rats

Main Article Content

Ansaf Saleh Abar
Saher Mahmood Jwad

Keywords

Zingiber officinale, Terpene , Alloxan, Diabetes mellitus, Antioxidants,MCP-1

Abstract

Current study was directed in AL- ameen center for research and advanced biotechnologies and the Faculty of science / branch of Natural science / animal dynasty, and 49 male albino rats be appropriate to the Sprague Dawley rinsing were charity. They assessed 200-245 g, and were distributed into seven clusters, 7 males in every cluster. The main encompassed regulator cluster that was verbally medicated with aqua of physiological salt 0.9% sodium chloride . The another cluster was vaccinated dermally with alloxan 100 mg/kg. The next and final clusters were orally administrated with terpene extract at the two concentrations (150,300) mg/kg respectively. The fifth and sixth clusters were vaccinated with alloxan 100 mg/kg, and then was orally dosed with terpene extract 150 and 300 mg/kg respectively. The last cluster was vocally acquiesced to the amyral solution 0.1 mg/kg after being injected with alloxan 100 mg/kg. It is worth mentioning that the dosage process continued for 30 days, just the once per day. The results of the statistical analysis chronicled a significant elevation (P < 0.05) in levels of blood glucose, malondialdehyde (MDA) and MCP-1 in the cluster vaccinating with alloxan hypodermically and also the cluster of alloxan +amyral when related with the regulator cluster and the additional investigational clusters, otherwise the planes of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) exposed a significant decrease (P˂ 0.05) in the two clusters mentioned . As for the clusters that were orally managed with terpene extract at the two concentrations 150 and 300 mg/kg revealed a notable reduction (P < 0.05) in the equal of blood glucose ,MDA and MCP-1 when equated with the regulator cluster then other study clusters .On the other hand, the levels of SOD and CAT and GPX1 exposed an observable increment (P˂ 0.05) in the cluster that treated with terpene quotation in the concentration 300 mg/kg in equated to the regulator cluster and other clusters of study.

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