Isolation and Identification of Pathogenic Escherichia coli from Different Sources in Najaf Hospital
Main Article Content
Keywords
piperacillin , lmipenem , aztreonam , VITEK-2
Abstract
This study aimed to isolate and diagnose Escherichia coli from patients with urinary tract, wounds and burns and its antibiotic resistance pattern. 100 samples were collected from patients with urinary tract infections, wounds and burns for both sexes and different ages from different hospitals in the city of Najaf for the period from the twenty-seventh of July 2022 to the eleventh of December 2023, and after the diagnosis. Samples in the laboratory with selective media, in addition to compressed VITEK-2, and the results showed that the incidence of urinary tract infection was 91% for males and females, wounds 7% for males and females, and burns 2% for males, and the results showed that the percentage of isolates resistance to piperacillin was 64 %, amoxicillin-clavulanate resistance 63%, ceftriaxone resistance rate 43%, ceftazidime resistance 33%, trimethoprim-sulfamethoxazole resistance 29%, levofloxacin resistance rate 25%, doxycycline resistance rate 20%, aztreonam resistance rate 19%, cefoxitin had a resistance rate of 15%, gentamicin had a resistance rate of 5%, lmipenem had a resistance rate of 1%, amikacin had a resistance rate of 1%.
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29. Mahmood N A. Synthesis of Antifungal Chemical Compounds from Fluconazole with (Pharma-Chemical) Studying.; Research journal of Pharmaceutical, biological and chemical sciences. 2017 ;8 (3), 564 -73
30. AL-Azawi, Y.R.S. (2015). Screening for cag Genes and Other Virulence Genes Among Escherichia coli Isolated from patients With Urinary Tract Infection In Hilla City. M. Sc. Thesis. Faculty of Medicine, University of Babylon.
31. Zahra Muhsin Ali.(2018). Horizontal gene conjugative transmission acquisition and transfer of antibiotic-resistance genes in bacteria. SCIENTIFIC JOURNAL OF MEDICAL RESEARCH. Vol. 2, Issue 6, pp 70-74, Spring 2018.
32. Alaq Saeed Abdulhussain. (2022). The efficacy and safety of vitamin C administration to women with history of premature preterm rupture of membrane in prevention of such event in current pregnancy: Randomized controlled clinical trial. Journal of Population Therapeutics and Clinical Pharmacology, 29(04), 188–194. https://doi.org/10.47750/jptcp.2022.985
33. Soumeia Zeghoud , Bachir Ben Seghir., Imane Kouadri , Hadia Hemmami , Abdelkrim Rebiai , Mohammed Messaoudi, Chinaza Godswill Awuchi. (2023) Classification of Plants Medicine Species from Algerian Regions using UV Spectroscopy, HPLC Chromatography, and Chemometrics Analysis , Malaysian Journal of Chemistry , Vol 25, No 1 ,126-142 ., DOI: https://doi.org/10.55373/mjchem.v25i1.126
34. Wan Mohd Nuzul Hakimi Wan Salleh .; Nurunajah Ab Ghani . (2022) Mini Review on Botany, Traditional Uses, Phytochemistry and Biological Activities of Piper amalago (Piperaceae)., Malaysian Journal of Chemistry ,Vol 24, No 4 , 201-208 ., DOI: https://doi.org/10.55373/mjchem.v24i4.201
35. Shweta A. More, Nikhil Sakle and Santosh N. Mokale . (2022) Design and Synthesis of N-4-(substituted benzylidene)-N-2-(4-chloropyrimidin-2-yl)-6, 7-dimethoxyquinazoline-2, 4-diamines as Anticancer Agents , Malaysian Journal of Chemistry , Vol 24, No 3 ,132-143., DOI: https://doi.org/10.55373/mjchem.v24i3.132
36. Tajbakhsh, E., Ahmadi, P., Abedpour-Dehkordi, E., Arbab-Soleimani, N., Khamesipour, F. (2016). Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrobial Resistance and Infection Control 5(11): 1 – 8. DOI: 10.1186/s13756-016-0109-4.
37. Salman, V., Bailey, J. V., Teske, A. (2013). Phylogenetic and morphologic complexity of giant sulphur bacteria. Antonie Van Leeuwenhoek. 104: 169–186.
38. Aparna, J., Pillai, P., & Ampili, M. (2021). ANTIBACTERIAL ACTIVITY OF Clerodendrum infortunatum L. AND Vanda spathulata (L.) Spreng. AGAINST Escherichia coli AND Klebsiella sp. Uttar Pradesh Journal of Zoology , 637-644.
39. Mattigatti, S., & Datkhile, K. (2021). Synthesis, characterization and application of green synthesized ag nps in endodontic therapy to reduce microbial biofilm. Uttar Pradesh Journal of Zoology,42(12), 25-30.
40. V. V. Vaidya . (2023). The Effects of Heavy Metal Contamination on Antioxidant Enzyme Activity and Oxidative Stress in the Earthworm Perionyx excavates . Uttar Pradesh Journal of Zoology,44(3), 59-65
2. McGraw-Hill education , Inc : 851pp.
3. Wanger, A.; Chavez, V.; Huang, R. S. P.; Wahed, A.; Actor, J. K. and Dasgupta, A. (2017). Microbiology and Molecular Diagnosis in Pathology. Elsevier Inc. All Rights Reserved. 300pp.
4. Oliveira, R. V.; Oliveira, M. C. and Pelli, A. (2017). Disease Infection by Enterobacteriaceae Family in Fishes: A Review. J Microbiol Exp. 4(5): 00128.
5. Parvez, S. A., and Rahman, D. (2018). Virulence Factors of Uropathogenic E. coli. Microbiology of Urinary Tract Infections. DOI: 10.5772/intechopen.79557
6. Russo TA, Johnson JR. Proposal for a new inclusive designation for extraintestinal pathogenic isolates of Escherichia coli: ExPEC. J Infect Dis 2000;181:1753- 1754.
7. Clinical and Laboratory Standard Institutes (2017). Performance Standard for Antimicrobial Susceptibility Testing . 27th ed . CSLISupplement M100. Wajne , PA : Clinical and Laboratory Standards Institute . 32-41
8. Belayneh Regasa Dadi, Tamrat Abebe, Lixin Zhang, Adane Mihret, Workeabeba Abebe .; Wondwossen Amogne. (2020) Distribution of virulence genes and phylogenetics of uropathogenic
9. Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia . BMC Infectious Diseases. 20:108
10. Themphachana M, Kongpheng S, Rattanachuay P, Khianngam S, Singkhamanan K, Sukhumungoon P. Molecular characterization of virulence and antimicrobial susceptibility profiles of uropathogenic Escherichia coli from patients in a tertiary hospital, southern Thailand. Southeast Asian J Trop Med Public Health. 2016;46(6):1021–1030.
11. Layla (2022). Acomparision study of antibacterial activity of Bacillus subtilis and silver nanoparticles against antibiotics – resistant E.coli isolated from different infections. Master thesis , College of sience , University of Kufa, Najaf , Iraq.
12. Horng, H. ; Chang, W. ; Yeh, C. ; Huang, B. ; Chang, C. ; Chen, Y. ; Tsui, K. and Wang, P. (2017). Estrogen Effects on Wound Healing. Int. J. Mol. Sci. 18(11): 2325.
13. Wilkinson, H. N. and Hardman, M. J. (2017). The role of estrogen in cutaneous ageing and repair. Maturitas.; 103:60–64.
14. Ekrami A, Kalantar E: Bacterial infections in burn patients at a burn hospital in India. Indian Journal of Medical Research, 126(6): 541-544, 2007.
15. Al-Hilali, S. A. H. (2010). Occurance and molecular characterization of enteropathogenic Escherichia coli ( EPEC ) serotypes isolates from children with diarrhea in Najaf .M.Sc.Thesis , Kufa University, College of Medicine
16. Al-Muhannak, F. H. (2010 ). Spread of some extended- spectrum B-Lactamases in clinical isolates of Gram – negative Bacilli in Najaf . M. Sc. Thesis , Kufa University , College of Medicin.
17. Luma Abd Almunim Baker, Shaymaa Zuhir Jalal Aldin. (2022). Association of some biochemical parameters and blood pressure among males with hypertension in the camps of Nineveh province-Iraq. Journal of Population Therapeutics and Clinical Pharmacology, 29(04), 167–176. https://doi.org/10.47750/jptcp.2022.979
18. Mostafavi, S.K.S.; Najar-Peerayeh S. ; Mobarez , A.M.& Parizi , M.K. (2019) . Serogroup Distribution , diversity of exotoxin gene profiles and phylogenetic grouping of CTX-M-1-producing uropathogenic Escherichia coli
comparative immunology , Microbiology and Infectious Diseases 65, 148-153.
19. Hadi, Z. J.(2008). Detection of extended-spectrum beta-lactamases of Escherichia coli and Klebsiella spp. Isolated from patients with significant bacteriuria in Najaf . M. Sc. Thesis , College of Medicine . Kufa University.
20. Mahmood N. A ., Zainab M. F. Anticancer Study of Innovative Macrocyclic Formazan Compounds from Trimethoprim Drug . Egyptian Journal of Chemistry ,2023, 66(1), DOI: 10.21608/EJCHEM.2022.132514.5852
21. Kafilzadeh, F.& Forsimadan , F. (2016) . Investigating Multidrug Efflux Pumps in Relation to the Antibiotic Resistance Pattern Escherichia coli Strains from Patients in Iran . Biomed Res . 27 (4), 1130-1135.
22. Asadi, S.; Kargar , M.; Solhjo , K. ;Najaf ,N. and Ghorbani – Dalini , S. (2014) . The Association of Virulence Determinants of Uropathogenic Escherichia coli with Antibiotic Resistance. Jundishapur J Microbiol .7(5), 1-5.
23. Anago, E.; Ayi-Fanou , L.; Akpovi, C. D.; Hounkpe, W.B.; Tchibozo, M. A. D.; Bankole, H. S. & Sanni, A. (2015). Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonov, Benin, Ann. Clin Microb. Anti., 14:5.
24. Ahmed, A. M. & Shimamator, T. (2008). Emergence of acefepime and cefpirome –resistant Citrobacter freundi clinical isolate harbouring anovel chromosomally encoded AmpC B-Lactamase , CMY – 37 .Int. J. Antimicrob . Agents , 32, 250-261.
25. Hadi, O.M. ; Al-Maliki , A.H. ; Al-Zubaidy , M.S.M.& Nihmah, Y.K. (2014) . Prevalence of Uro pathogenic Escherichia coli in Al-Hashymia District of Babylon Province . JUBPAS . 9(22), 2479-2488.
26. Ramirez- Castillo , F. Y. ; Moreno-Flores , A. C. ; Avelar-Gonzalez , F. J. ; Marquez-Diaz , F. ; Harel , J. & Guerrero- Barrera , A. L. (2018) . An Evaluation of Multidrug- Resistant Escherichia coli Isolates in Urinary Tract Infections from Aguascalientes , Mexico : Cross-Sectional Study . Ann Clin Microbiol Antimicrob . 17 (34), 1-13.
27. Paltansing , S. (2015) .Antimicrobial Resistance in Enterobacteriaceae : Characterization and Dedection . Leiden University . 155.
28. Polse, R., Yousif, S., and Assafi, M. (2016). Prevalence and antimicrobial susceptibility patterns of uropathogenic E. coli among people in Zakho, Iraq. International Journal of Research in Medical Sciences, December, 1219–1223. ,DOI: 10.18203/2320-6012.ijrms20160813
29. Mahmood N A. Synthesis of Antifungal Chemical Compounds from Fluconazole with (Pharma-Chemical) Studying.; Research journal of Pharmaceutical, biological and chemical sciences. 2017 ;8 (3), 564 -73
30. AL-Azawi, Y.R.S. (2015). Screening for cag Genes and Other Virulence Genes Among Escherichia coli Isolated from patients With Urinary Tract Infection In Hilla City. M. Sc. Thesis. Faculty of Medicine, University of Babylon.
31. Zahra Muhsin Ali.(2018). Horizontal gene conjugative transmission acquisition and transfer of antibiotic-resistance genes in bacteria. SCIENTIFIC JOURNAL OF MEDICAL RESEARCH. Vol. 2, Issue 6, pp 70-74, Spring 2018.
32. Alaq Saeed Abdulhussain. (2022). The efficacy and safety of vitamin C administration to women with history of premature preterm rupture of membrane in prevention of such event in current pregnancy: Randomized controlled clinical trial. Journal of Population Therapeutics and Clinical Pharmacology, 29(04), 188–194. https://doi.org/10.47750/jptcp.2022.985
33. Soumeia Zeghoud , Bachir Ben Seghir., Imane Kouadri , Hadia Hemmami , Abdelkrim Rebiai , Mohammed Messaoudi, Chinaza Godswill Awuchi. (2023) Classification of Plants Medicine Species from Algerian Regions using UV Spectroscopy, HPLC Chromatography, and Chemometrics Analysis , Malaysian Journal of Chemistry , Vol 25, No 1 ,126-142 ., DOI: https://doi.org/10.55373/mjchem.v25i1.126
34. Wan Mohd Nuzul Hakimi Wan Salleh .; Nurunajah Ab Ghani . (2022) Mini Review on Botany, Traditional Uses, Phytochemistry and Biological Activities of Piper amalago (Piperaceae)., Malaysian Journal of Chemistry ,Vol 24, No 4 , 201-208 ., DOI: https://doi.org/10.55373/mjchem.v24i4.201
35. Shweta A. More, Nikhil Sakle and Santosh N. Mokale . (2022) Design and Synthesis of N-4-(substituted benzylidene)-N-2-(4-chloropyrimidin-2-yl)-6, 7-dimethoxyquinazoline-2, 4-diamines as Anticancer Agents , Malaysian Journal of Chemistry , Vol 24, No 3 ,132-143., DOI: https://doi.org/10.55373/mjchem.v24i3.132
36. Tajbakhsh, E., Ahmadi, P., Abedpour-Dehkordi, E., Arbab-Soleimani, N., Khamesipour, F. (2016). Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrobial Resistance and Infection Control 5(11): 1 – 8. DOI: 10.1186/s13756-016-0109-4.
37. Salman, V., Bailey, J. V., Teske, A. (2013). Phylogenetic and morphologic complexity of giant sulphur bacteria. Antonie Van Leeuwenhoek. 104: 169–186.
38. Aparna, J., Pillai, P., & Ampili, M. (2021). ANTIBACTERIAL ACTIVITY OF Clerodendrum infortunatum L. AND Vanda spathulata (L.) Spreng. AGAINST Escherichia coli AND Klebsiella sp. Uttar Pradesh Journal of Zoology , 637-644.
39. Mattigatti, S., & Datkhile, K. (2021). Synthesis, characterization and application of green synthesized ag nps in endodontic therapy to reduce microbial biofilm. Uttar Pradesh Journal of Zoology,42(12), 25-30.
40. V. V. Vaidya . (2023). The Effects of Heavy Metal Contamination on Antioxidant Enzyme Activity and Oxidative Stress in the Earthworm Perionyx excavates . Uttar Pradesh Journal of Zoology,44(3), 59-65
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Apr 26, 2023
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Rusul Najm, & Jinan Mohammed Hussein. (2023). Isolation and Identification of Pathogenic Escherichia coli from Different Sources in Najaf Hospital. Journal of Population Therapeutics and Clinical Pharmacology, 30(8), 459–464. https://doi.org/10.47750/jptcp.2023.30.08.049
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