Ca Ionophore Vs. Standard Culture Media In Cryopreserved Semen Of Iraqi Patients Undergoing ICSI Cycles

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Manar Alhoda Zaid Al-Faqheri
Manal Taha Al-Obaidi
Ula Al-Kawaz


Artificial oocyte activation, in vitro fertilization, intracytoplasmic sperm injection


Background: ICSI is increasingly used instead of traditional IVF for all IVF-using couples for several reasons. It has already successfully activated mammalian oocytes with an artificial oocyte activator called calcium ionophores. There is a growing trend to use such activators to address the many covert causes of ineffective fertilization and improve embryo quality. The fear that fertilization might not happen is one of the most important ones.
Aim of the study: to analyze the effects of artificial oocyte activation on the outcomes of ICSI procedure when cryopreserved semen is used.
Material and methods: A total of 17 patients who were selected and subjected to intra-cytoplasmic sperm injection procedure, after controlled ovarian stimulation and ovum pick oocyte immediately following Intracytoplasmic sperm injection were divided into two groups (oocyte with ca ionophore and without ca ionophore) then fertilization rate and embryo quality were checked to each group.
Result: The study involved 162 oocytes, 83 in the ca ionophore group and 79 in the control group. The fertilization rate did not show any noteworthy difference between the two groups. Moreover, there was no significant statistical difference between the two groups regarding embryo grades 1, 2, and 3.
Conclusion: Artificial oocyte activation did not benefit fertilization rate nor embryo quality when cryopreserved semen was used for insemination.

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