Correlation between (Procalcitonin, CRP and interleukin-6) with cystatin C in type-2 diabetes mellitus patients

Abstract

All nucleated cells consistently synthesize and secrete cystatin C, a basic protein that is not glycosylated. Because it is less influenced by outside variables including gender, race, and muscle mass, cystatin C is a more trustworthy measure than blood creatinine. Serum cystatin C levels in type 2 diabetes mellitus are not well understood in individuals with obesity, nevertheless. A low-molecular weight protein called cystatin C that is readily filtered by the kidneys has just come to light as a potential biomarker that may be utilized to identify early renal failure in people with type 1 or type 2 diabetes. Additionally, it has been demonstrated that cystatin C can be used to identify cardiovascular disease in diabetics, and it may possibly be connected to the occurrence of type ll diabetes in obese individuals. This study aimed correlation cystatin C (Cys C) levels in type ll diabetes and Procalcitonin , CRP and interleukin-6 .inflammatory marker
Material and methods: the blood samples of Type II diabetes mellitus (30 cases) and control (30 normal) groups were collected in private laboratory through the study dated between February 2022 and june 2022. HbA1c test was done immediately then the blood sample was centrifuged. Serum divided into two part. The first part was used for the following test : random blood sugar, total protein, albumin, GPT, GOT, ALP. The other was refrigerated for Procalcitonin , CRP, interleukin-6 and cystatin C tests by using enzyme-linked immunosorbent assay (ELISA).
Results: The mean cystatin-c levels were increased in Type II diabetes patients significantly (P≤ 0.0001) compared with healthy groups (2.55±1.12 pg/ml, 0.55±0.21pg/ml ) respectively. Also, CRP ,IL-6 and procalcitonin levels were bigger in Type II diabetes patients significantly (P > 0.0001) in Type 2 diabetes linked with healthy groups (30.45±16.4 ng/ml, 2.19±1.27 ng/ml , 16.19±4.32 ng/ml 6.06±3.10 and 4.92±4.00 , 0.315± 0.22 ) respectively.
Conclusions: According to our research, individuals with Type II diabetes have circulating Cys C levels that are higher than those of healthy individuals. To determine the cause of abnormal Cys C concentrations in Type II diabetes, more research is still required.