Evaluating the biological activity of lemongrass and rosemary essential oils against some fungi isolated from vegetables and fruits
Main Article Content
Keywords
lemongrass , rosemary, antifungals, fungal growth, essential oils, vegetables, fruits
Abstract
Five dominant species of fungi were identified by morphological and molecular methods (Aspergillus niger, Penicillium fimorum , Penicllium digtaum, Sclerotinia sclerotiorum , Alternaria alternate. our study showed the antifungal effects of lemongrass(Cymbopogon citratus L) and rosemary(Rosmarinus officinalis L), essential oils (E.O) against the identified fungi which were determined in vitro conditions. Different concentrations were tested ( 500, 1000.1500) ppm. 1500ppm of lemongrass E.O showed 100% inhibitory effect against the tested fungi, whereas Rosemary (E.O) were less effective at the same concentration. The essential oils of lemongrass showed significant inhibitory effects against Aspergillus niger , Alternaria alternate when compared with rosemary oil. While the effect of rosemary oil had a clear effect on both Penicillium fimorum and Penicllium digtaum. In general, all tested essential oils showed. Inhibitory effect against all selected fungi
References
1. Sadiq Obeis Rasan, 2017, Formation and diagnosis of mycorrhizal fungi For the leaves of leafy crops in Diwaniyah spend your chastity, College of Science, Department of Life Sciences, Al-Qadisiyah University.
2. Ajayi A.O., Okoh A.I., (2013( .Antimycotic activity of some medicinal plants on aquatic fungi, European Journal of Experimental Biology, pp. 16-21.
3. Rasime Demirel Nalan Yilmaz Sariozlu Semra İlhan, ( 2013 ) Polymerase chain reaction (PCR)
identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province, Biological and Applied Sciences • Braz. arch. biol. technol. 56 (6).
4. Taha, Heba , Sharif Adiba, Tahir Amyan , ( .
2020 ) Isolation and identification of the fungi that cause rotting of green pepper fruits and study of their ability to produce Protease and Lipase enzymes, Arab Journal of Agricultural Sciences Volume Three, Issue 6, p. 61.
5. Al-Harithi, Asmaa Ahmed, Ramadan Nadim Ahmed, ( . 2009 ) Isolation and identification of species belonging to the genus Penicillium from indoor dust of buildings in the city of Mosul / Iraq, Al-Rafidain Science Journal, Vol. 20 1 for issue 1, pp. 1-10, 2009, p. 5.
6. Mahilrajan S., Nandakumar J., Kailayalingam R., Monoharan N.A., Srivijeindran S., (. 2014 )
Screening the antifungal activity of essential oils against decay fungi from palmyrah leaf handicrafts, Biological Research, 47 , p. 35.
7. Kishore N, Mishra AK, Chansouria JP. ( 1993 )
Fungitoxicty of essential oils against dermatophyts. Mycoses.;36:211–5.
8. Abe S, Sato Y, Inoue S, Ishibashi H, Maruyama N, Takizawa T, et al. ( . 2003 ) Anti-Candida albicans activity of essential oils including Lemongrass (Cymbopogon citratus Nippon Ishinkin Gakkai Zasshi. ;44:285–91.
9. Wannissorn B, Jarikasem S, Soontorntanasart T. .
( 1996 ) Antifungal activity of lemon grass and lemon grass oil cream. Phytother Res.;10:551–4.
10. Renata H. Esper , Edlayne Gonçalez , Marcia O. M. Marques , Roberto C. Felicio and Joana D. Felicio , ( . 2014 ) Potential of essential oils for protection of grains contaminated by aflatoxin produced by Aspergillus flavus, Front. Microbiol., 04 June.
11. Banwart., G., Basic Food Microbiology, Springer Shop, )2012(,1517.
12. Bennett, J. W. and Klich, M. Mycotoxins, Clin Microbiol Rev. 2003 Jul; 16(3): 497–516.
13. Baz, Ahmed Mohammad, Rasha Mosbah, Reham M. Goda , ( . 2021 ) Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils, Antibiotics 10(1):81,.
14. Agarwal, V.; Lal, P.; Pruthi, V. ( . 2008 )
Prevention of Candida albicans biofilm by plant oils. Mycopathologia, 165, 13–19.
15. Kadry, A.A.; El-Ganiny, A.M.; El-Baz, A.M. ( .2018 ) Relationship between Sap prevalence and biofilm formation among resistant clinical isolates of Candida albicans. Afr. Health Sci., 18, 1166–1174
16. Voda K., B. Boh, Vrtacnik M., Pohleven F., ( .2003 ) Effect of the antifungal activity of oxygenated aromatic essential oil compounds on the white-rot Trametes versicolor and the Brown-not Coniophora puteana, International Biodeterioration and Biodegradation, 51, pp. 51-59.
17. Stevi A., Beri C., Katarina S., Marina S., Dejan G., ( . 2014 ) Antifungal activity of selected essential oils against fungi isolated from medicinal plant
18. Stupar M., Grbic M.L., Dzamic A., Unkovic N., M. Ristic, ( . 2014 ) Antifungal activity of selected essential oils and biocide benzalkonium chloride against the fungi isolated from cultural heritage objects, South African Journal of Botany, 93, pp. 118-124.
19. Bennett, J. W. and Klich, M. ( . 2003 ) Mycotoxins, Clin Microbiol Rev. Jul; 16(3): 497–516.
20. Abdullah, S.K. and S.M, AL-Bader. 1990. On the Thermophilic and Thermotolerant Mycoflora of Iraqi Soils. Sydowia. 42 .pp.17.
21. Wang, X. C., Liu, C., Huang, L., Bengtsson-Palme, J., Chen, H., Zhang, J. H., ... & Li, J. Q. (2015). ITS 1: a DNA barcode better than ITS 2 in eukaryotes?. Molecular ecology resources, 15(3), 573-586.
22. Mohammad Moghaddam, Leila Mehdizadeh, ( .2016 ) Essential Oil and Antifungal Therapy .
23. Moghtader M .H Salari,A Farahmand, (. 2011 ) Evaluation of the antifungal effects of rosemary oil and comparison with synthetic borneol and fungicide on the growth of Aspergillus flavus .
24. Pitt, J.I. and Hocking A.D. )1997(. Fungi and Food Spoilage. 2nd. Gaithersburg, Maryland: Chapman and Hall. 593 p.
25. Kurtzman, C. P., Robnett, C. J., Basehoar, E., & Ward, T. J. (2018). Four new species of Metschnikowia and the transfer of seven Candida species to Metschnikowia and Clavispora as new combinations. Antonie van Leeuwenhoek, 111(11), 2017-2035Mahaadeven,A.1982.Biochemical aspects of plant disease resistance .Part 1 .Preformed inhibitory substances (prohibitins).Today and Tomorrow ,s Printers and publishers,New Delhi,India
26. Gabler, F.M, M.F. Mansour, J.L. Smilanick and B. E., Mackey. )2004(. Survival of spores of Rhizopus stolonifer, Aspergillus niger, Botrytis cinerea and Alternaria alternate after exposure to ethanol solutions at various temperatures. Journal of Appliction Microbiology. 96(6): 1354-1360.
27. Rushd Radwan Khalifa, Niran Salem Al-Jarrah, Uday Najm Ismail Matani, ( . 2017 ) Detection and investigation of contamination with the fungus Aspergillus niger A and the marking of Okra A in walnuts and pistachios, Iraqi Journal of Agricultural Sciences - 1223-1230(: 5)48 .
28. Renata H. Esper , Edlayne Gonçalez , Marcia O. M. Marques , Roberto C. Felicio and Joana D. Felicio , ( . 2014 ) Potential of essential oils for protection of grains contaminated by aflatoxin produced by Aspergillus flavus, Front. Microbiol., 04 .
29. Banwart., G., ( . 2012 ) Basic Food Microbiology, Springer Shop,1517.
30. Industrial Crop Production, 55, pp. 116-122.
31. Pitt John,I. and Ailsa,D.Hocking(2009)Fungi and food spoilage,p.519.
2. Ajayi A.O., Okoh A.I., (2013( .Antimycotic activity of some medicinal plants on aquatic fungi, European Journal of Experimental Biology, pp. 16-21.
3. Rasime Demirel Nalan Yilmaz Sariozlu Semra İlhan, ( 2013 ) Polymerase chain reaction (PCR)
identification of terverticillate Penicillium species isolated from agricultural soils in eskişehir province, Biological and Applied Sciences • Braz. arch. biol. technol. 56 (6).
4. Taha, Heba , Sharif Adiba, Tahir Amyan , ( .
2020 ) Isolation and identification of the fungi that cause rotting of green pepper fruits and study of their ability to produce Protease and Lipase enzymes, Arab Journal of Agricultural Sciences Volume Three, Issue 6, p. 61.
5. Al-Harithi, Asmaa Ahmed, Ramadan Nadim Ahmed, ( . 2009 ) Isolation and identification of species belonging to the genus Penicillium from indoor dust of buildings in the city of Mosul / Iraq, Al-Rafidain Science Journal, Vol. 20 1 for issue 1, pp. 1-10, 2009, p. 5.
6. Mahilrajan S., Nandakumar J., Kailayalingam R., Monoharan N.A., Srivijeindran S., (. 2014 )
Screening the antifungal activity of essential oils against decay fungi from palmyrah leaf handicrafts, Biological Research, 47 , p. 35.
7. Kishore N, Mishra AK, Chansouria JP. ( 1993 )
Fungitoxicty of essential oils against dermatophyts. Mycoses.;36:211–5.
8. Abe S, Sato Y, Inoue S, Ishibashi H, Maruyama N, Takizawa T, et al. ( . 2003 ) Anti-Candida albicans activity of essential oils including Lemongrass (Cymbopogon citratus Nippon Ishinkin Gakkai Zasshi. ;44:285–91.
9. Wannissorn B, Jarikasem S, Soontorntanasart T. .
( 1996 ) Antifungal activity of lemon grass and lemon grass oil cream. Phytother Res.;10:551–4.
10. Renata H. Esper , Edlayne Gonçalez , Marcia O. M. Marques , Roberto C. Felicio and Joana D. Felicio , ( . 2014 ) Potential of essential oils for protection of grains contaminated by aflatoxin produced by Aspergillus flavus, Front. Microbiol., 04 June.
11. Banwart., G., Basic Food Microbiology, Springer Shop, )2012(,1517.
12. Bennett, J. W. and Klich, M. Mycotoxins, Clin Microbiol Rev. 2003 Jul; 16(3): 497–516.
13. Baz, Ahmed Mohammad, Rasha Mosbah, Reham M. Goda , ( . 2021 ) Back to Nature: Combating Candida albicans Biofilm, Phospholipase and Hemolysin Using Plant Essential Oils, Antibiotics 10(1):81,.
14. Agarwal, V.; Lal, P.; Pruthi, V. ( . 2008 )
Prevention of Candida albicans biofilm by plant oils. Mycopathologia, 165, 13–19.
15. Kadry, A.A.; El-Ganiny, A.M.; El-Baz, A.M. ( .2018 ) Relationship between Sap prevalence and biofilm formation among resistant clinical isolates of Candida albicans. Afr. Health Sci., 18, 1166–1174
16. Voda K., B. Boh, Vrtacnik M., Pohleven F., ( .2003 ) Effect of the antifungal activity of oxygenated aromatic essential oil compounds on the white-rot Trametes versicolor and the Brown-not Coniophora puteana, International Biodeterioration and Biodegradation, 51, pp. 51-59.
17. Stevi A., Beri C., Katarina S., Marina S., Dejan G., ( . 2014 ) Antifungal activity of selected essential oils against fungi isolated from medicinal plant
18. Stupar M., Grbic M.L., Dzamic A., Unkovic N., M. Ristic, ( . 2014 ) Antifungal activity of selected essential oils and biocide benzalkonium chloride against the fungi isolated from cultural heritage objects, South African Journal of Botany, 93, pp. 118-124.
19. Bennett, J. W. and Klich, M. ( . 2003 ) Mycotoxins, Clin Microbiol Rev. Jul; 16(3): 497–516.
20. Abdullah, S.K. and S.M, AL-Bader. 1990. On the Thermophilic and Thermotolerant Mycoflora of Iraqi Soils. Sydowia. 42 .pp.17.
21. Wang, X. C., Liu, C., Huang, L., Bengtsson-Palme, J., Chen, H., Zhang, J. H., ... & Li, J. Q. (2015). ITS 1: a DNA barcode better than ITS 2 in eukaryotes?. Molecular ecology resources, 15(3), 573-586.
22. Mohammad Moghaddam, Leila Mehdizadeh, ( .2016 ) Essential Oil and Antifungal Therapy .
23. Moghtader M .H Salari,A Farahmand, (. 2011 ) Evaluation of the antifungal effects of rosemary oil and comparison with synthetic borneol and fungicide on the growth of Aspergillus flavus .
24. Pitt, J.I. and Hocking A.D. )1997(. Fungi and Food Spoilage. 2nd. Gaithersburg, Maryland: Chapman and Hall. 593 p.
25. Kurtzman, C. P., Robnett, C. J., Basehoar, E., & Ward, T. J. (2018). Four new species of Metschnikowia and the transfer of seven Candida species to Metschnikowia and Clavispora as new combinations. Antonie van Leeuwenhoek, 111(11), 2017-2035Mahaadeven,A.1982.Biochemical aspects of plant disease resistance .Part 1 .Preformed inhibitory substances (prohibitins).Today and Tomorrow ,s Printers and publishers,New Delhi,India
26. Gabler, F.M, M.F. Mansour, J.L. Smilanick and B. E., Mackey. )2004(. Survival of spores of Rhizopus stolonifer, Aspergillus niger, Botrytis cinerea and Alternaria alternate after exposure to ethanol solutions at various temperatures. Journal of Appliction Microbiology. 96(6): 1354-1360.
27. Rushd Radwan Khalifa, Niran Salem Al-Jarrah, Uday Najm Ismail Matani, ( . 2017 ) Detection and investigation of contamination with the fungus Aspergillus niger A and the marking of Okra A in walnuts and pistachios, Iraqi Journal of Agricultural Sciences - 1223-1230(: 5)48 .
28. Renata H. Esper , Edlayne Gonçalez , Marcia O. M. Marques , Roberto C. Felicio and Joana D. Felicio , ( . 2014 ) Potential of essential oils for protection of grains contaminated by aflatoxin produced by Aspergillus flavus, Front. Microbiol., 04 .
29. Banwart., G., ( . 2012 ) Basic Food Microbiology, Springer Shop,1517.
30. Industrial Crop Production, 55, pp. 116-122.
31. Pitt John,I. and Ailsa,D.Hocking(2009)Fungi and food spoilage,p.519.
Article Sidebar
Published
Apr 18, 2023
Article Details
How to Cite
Sara J. Jalel, Ibrahim J. Abed, & Aktham N. Jasim. (2023). Evaluating the biological activity of lemongrass and rosemary essential oils against some fungi isolated from vegetables and fruits. Journal of Population Therapeutics and Clinical Pharmacology, 30(8), 46–54. https://doi.org/10.47750/jptcp.2023.30.08.005
Section
Articles
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.