Isolation and diagnosis of Streptococcus mutans and Enterococcus faecalis from dental caries patients in Thi- Qar Governorate

Main Article Content

Emaduldeen Hatem Abed
Mohammed Hashim Al-Yasiri
Afrah Abid Maktoof


Dental caries, Streptococcus mutans, Enterococcus faecalis


This investigation was conducted to isolate Streptococcus mutans and Enterococcus faecalis from dental caries patients in Thi-Qar governorate. A total of (100) samples were gathered from people aged 4 to 65 with the help of a dentist, samples were taken with aseptic techniques. The samples were transferred by specially sterilized containers. Traditional and molecular method were used for isolation and diagnosis bacterial isolates. Total of Forty-five (45%) samples were identified as Streptococcus mutans followed by 25 (25) isolates identified as Enterococcus faecalis.

Abstract 442 | pdf Downloads 82


1. Tanner, A. C. R., Kressirer, C. A., Rothmiller, S., Johansson, I., & Chalmers, N. I. (2018). The caries microbiome: implications for reversing dysbiosis. Advances in dental research, 29(1), 78-85.
2. Yadav, K., & Prakash, S. (2017). Dental caries: A microbiological approach. J Clin Infect Dis Pract, 2(1), 1-15.
3. Bowen, W. H., Burne, R. A., Wu, H., & Koo, H. (2018). Oral biofilms: pathogens, matrix, and polymicrobial interactions in microenvironments. Trends in microbiology, 26(3), 229-242.
4. Li, J., Wu, T., Peng, W., & Zhu, Y. (2020). Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans. BMC microbiology, 20(1), 1-11.
5. Gross, E. L., Beall, C. J., Kutsch, S. R., Firestone, N. D., Leys, E. J., & Griffen, A. L. (2012). Beyond Streptococcus mutans: dental caries onset linked to multiple species by 16S rRNA community analysis.
6. Pinheiro, E. T., Gomes, B. P. F. A., Ferraz, C. C. R., Teixeira, F. B., Zaia, A. A., & Souza Filho, F. J. (2003). Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility. Oral microbiology and immunology, 18(2), 100-103.
7. Rodríguez-Niklitschek, C., & Oporto V, G. H. (2015). Clinical implications of Enterococcus faecalis microbial contamination in root canals of devitalized teeth: Literature review. Revista odontológica mexicana, 19(3), 181-186.
8. Al-Mizrakchi, A. (1998). Adherence of mutans Streptococci on teeth surfaces: microbiological and biochemical studies (Doctoral dissertation, Phd thesis, University of Al-Mustansiriya, Iraq).
9. Hirasawa, M., & Takada, K. (2002). Susceptibility of Streptococcus mutans and Streptococcus sobrinus to cell wall inhibitors and development of a novel selective medium for S. sobrinus. Caries research, 36(3), 155-160.
10. Bassa, S., Workie, S. B., Kassa, Y., & Tegbaru, D. W. (2023). Prevalence of dental caries and relation with nutritional status among school-age children in resource limited setting of southern Ethiopia. BMC Oral Health, 23(1), 84.
11. Vishnu, R. A., Alamelu, S., Arun, K. V., Sujitha, P., & Ganesh, P. R. (2022). Comparative evaluation of subgingival microbiome in healthy periodontium and gingivitis using next-generation sequencing technology: A case–control study. Journal of Indian Society of Periodontology, 26(3), 224.
12. P., Turagam, N., & Ealla, K. K. (2018). Molecular detection of Porphyromonas gingivalis in chronic periodontitis patients. J Contemp Dent Pract, 19(8), 992-6.
13. Abd Al-Zahra, H. S., & Saleh, M. B. (2018). Isolation and identification ofStreptococcus mutans from dental caries patients at Thi-Qar province/Iraq. journal of THI-QAR science, 6(4).
14. Costa Oliveira, B. E., Ricomini Filho, A. P., Burne, R. A., & Zeng, L. (2021). The route of sucrose utilization by Streptococcus mutans affects intracellular polysaccharide metabolism. Frontiers in microbiology, 12, 636684.
15. Mohammed, R. H., & Al Niaame, A. E. (2022). Isolation and identification of some species of pathogenic bacteria from Periodontitis, dental caries, and root canal inflammation, and the detection of some factors of their virulence. journal of the college of basic education.
16. Karayasheva, D., & Radeva, E. (2017). Importance of Enterococci (Enterococcus faecalis) for Dental Medicine–Microbiological Characterization, Prevalence and Resistance. Int J Sci Res, 6, 1970-1973.
17. Paganelli, F. L., Willems, R. J., & Leavis, H. L. (2012). Optimizing future treatment of enterococcal infections: attacking the biofilm?. Trends in microbiology, 20(1), 40-49.