Assessment of Beta-lactamases and Integrons Genes among Bacteria Isolated From Bladder Cancer Patients with Urinary Tract Infections
Main Article Content
Keywords
MBLs genes ,ESBLs genes , Integrons , Bladder Cancer ,UTI
Abstract
Background and Aim: One of the most dangerous side effects and the main factor in both morbidity and mortality in cancer patients are infections. Urinary tract infections (UTIs) are among the most prevalent infections in cancer patients. The Recent study aimed to isolation , identification of bacteria from bladder cancer patients with UTIs treated with BCG OR Mitomycin C (MMC) and characterized the presence of bla SHV , bla IMP by PCR in addition to detection the prevalence of Integron gene in the isolated bacterium .
Methods: Two hundred urine samples were taken from patients with bladder cancer one hundred from bladder cancer patients treated with BCG (Group-1) and one hundred treated with Mitomycin C (Group –II) between the dates of 1 April 2021 and 15 October 2021 while they were enrolled in third floor Ghazi Al- Hariri Hospital, Medical City, Baghdad Province, and private clinics in Nasiriyah Province. Forty urine samples were taken from patients with UTI without Bladder Ca (Control ,Group -III). Bacterial strains were identified using the Indole test ,Oxidase test , β-hemolytic activity, API20 E test and by 16sRNA .
Results: The most commonly pathogens were Escherichia coli , followed by Klebsiella pneumoniae , Pseudomonas aeruginosa and Staphyllococcus aureus and other genera of UTI bacterium. The total BlaSHV Producers in the current study from 38 isolates were 18 isolate(47.3%).The highest BlaSHV Producer in all Groups was E.coli followed by K.pneumoniae and P.aeruginosae . None of all tested isolates were positive to BlaIMP gene while the presence of Integron class 1 was detected in( 34 )89.4% of total isolates.
References
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11. Anoar, KA., Ali, FA. & Omer, SA. Detection of metallo [Beta]-lactamase enzyme in somegram negative bacteria isolated from burn patients in Sulaimani city, Iraq. Eur Sci J. 2014, 10(3),485–496.
12. Mojica, M.F., Bonomo, R.A.& Fast, W. B1-Metallo-Beta-Lactamases: Where Do We Stand? Curr Drug Targets,2016, 17, 1029–1050. [CrossRef]
13. Correa, FE., Dantas, FG., Grisolia, AB., CrispimBdo, A.& Oliveira, KM. Identification of class 1 and 2 integrons from clinical and environmental Salmonella isolates. J Infect Dev Ctries;2014, 8:1518-1524.
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17. Shaebth ,L.J. . Molecular identification and sequencing of Pseudomonas aeruginosa virulence genes among different isolates in Al-Diwaneyah hospital . Iraqi Journal of Veterinary Sciences,2018, 32 ( 2) : 183-188.
18. Amine, A.E.K. Extended spectrum beta-lactamase producing bacteria in waste water alexandria, Egypt. Int. J. Biosci. Biochem. Bioinform.2013, 3, 605.
19. Haji,S.H., Jalal,S.T., Omer,S.A. and MawloodA.H. Molecular detection of SHV-Type ESBL in E. coli and K.pneumoniae and their antimicrobial resistance profile. Zanco J. Med. Sci.,2018, Vol. 22, No. (2). https://doi.org/10.15218/zjms.2018.035
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21. Carroll, K. C. , Hobden, J. A. , Miller, S., Morse, S. A., Mietzner, T. A., Detrick, B., … .. Jawetz, Melnick and Adelbergs Medical Microbiology27th ed.2016, McGraw-Hill Education.USA.
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25. Martínez-Delgado, G., Garza-Gangemi,A.M. & Castillejos-Molina,R.A. Urinary tract infections after transurethral resection of the bladder: Microbiology, antibiotic resistance, and associated risk factors. Microbiology, antibiotic resistance and associated risk factors. Rev. Mex. Urol.2020, 80(4):pp 1-12
26. Al-Hamadani ,A.H., Al-Rikabi,A.M. & AlFatlawi ,A.F. Detection of TEM and SHV genes in Escherichia coli and Klebseilla species isolated from cancer patients in Al-Diwaniya Governorate. QMJ .2013,9(16 ).22.
27. Arshad ,S.Z& Yousaf ,A..Determination of antibiotic susceptibility patterns in urinary tract infections among Cancer patients. Türk Fizyoterapi ve Rehabilitasyon Dergisi/Turkish
Journal of Physiotherapy and Rehabilitation 2021.
28. Lhwak ,N.S.&Abbas ,Y.A. Detection of Extended Spectrum β–Lactmase GeneeCTX-M-1 in Escherchia coli and Klebseilla pneumonia Isolated from Urinary Tract Infection of Pregnant Women in Al Nassyriah City. .J.Thi-Qar Sci.2018, 6(4) .92.
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Apr 18, 2023
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Huda J. Mohemmad, Yahya A. Abbas Alkhafaji, & Hazim R.Alkafaji. (2023). Assessment of Beta-lactamases and Integrons Genes among Bacteria Isolated From Bladder Cancer Patients with Urinary Tract Infections. Journal of Population Therapeutics and Clinical Pharmacology, 30(8), 9–23. https://doi.org/10.47750/jptcp.2023.30.08.002
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