Detection the Phylogenetic groups of E. coli that isolated from diarrheal in children under five years and study their relationship of common serotypes in Baghdad hospitals

Main Article Content

Shahla Najim Abed Al-Azzawi
Rana Mujahid Abdullah

Keywords

diarrhea, E. coli, serological test, phyllo-groups

Abstract

The study included 50isolates of E.coli isolatesthat were obtained from (120) stool samples collected from children with diarrhea of both sexes who visited hospitals in Baghdad. E.coli serotypes were diagnosed according to serotyping method for different age groups of children under five years. The results were represented by (7) (14%) isolates of E.coli belonging to Anticoli 1 group, (10) (20%) isolates belonging to Anticoli 11 group, (5) (23%) isolates belonging to Anticoli 111 group and (22) (44%) isolates which included (2/22) (9%)belongs to serotypes O111, O78 by (2/22) (9%), and for each of the serotypes O25, O142, and O119 are (3/22) and by 14%, O55 (5/22) ) at 23% and O44 (4/22) by 18%. As for the rest of the isolates, no results were given, which is represented by (28) (56%) unknown isolates which were not classified for any of the serotypes. Moreover, the results showed that the highest percentage of diarrhea was in males at (56%), while in females it was(44%); in addition the highest percentage of infection with E. coli in males and females was within the age group (1-12) months by (48%), followed by the age group of (12-24) months by (28%), and then the age group (36-48) by 14%, while the lowest rate of E.coli infection in males and females found in the age group (24-36) months by (6%) followed by the age group (48-60) monthsby(4%).The results of the phylogenetic groups detected several groups includinggroup A (2/50, 4%), B1 (0/50, 0%), B2 (1/50, 2%), C (12/50, 24%), D (6/50,12%), F (3/50,6%), Clade1 (0%) and E (26/50, 52%) which is the predominant group.

Abstract 244 | PDF Downloads 165

References

1. Abdul-Razzaq MS , Abdul-Lateef LA .( 2011).Molecular phylogeny of Escherichia coli isolated from clinical samples in Hilla. Iraq. Afr J Biotechnol;10:15783–7 .
2. Al-Kalifawi, E. J.;( 2013). Bacterial Causes Conjunctivitis in Children, Study the Resistance to Antimicrobials and The Effect of Breast Milk on Isolated Bacteria. . Ibn Al-Haitham Jour. for Pure & Appl. Sci. University of Baghdad .Vol. 26 (2).8-17.
3. Alfinete, N.W., Bolukaoto, J.Y., Heine, L., Potgieter, N. and Barnard, T.G.,( 2022). Virulence and phylogenetic analysis of enteric pathogenic Escherichia coli isolated from children with diarrhoea in South Africa. International Journal of Infectious Diseases, 114, pp.226-232.
4. Abdul-Ghaffar, S.N. and Abu-Risha, R.A., (2017) . Virulence Genes Profile of Escherichia coli Isolated from Urinary Catheterized and Non-Catheterized Patients. Iraqi Journal of Science, pp.820-835.
5. Ibrahim, S. K., & Hadi, T. F. (2015). Bacteriological and Immunological Study of Rheumatoid Arthritis. International Journal of Science and Research, 6(7), 1483-1487.
6. Khalil, Z.K., (2016). Isolation and identification of Staphylococcus aureus, Listeria monocytogenes, E. coli O157: H7 and Salmonella specious from raw beef and lamb meat in Baghdad by PCR. Iraqi Journal of Science..
7. Abdullah, R.M., 2016. A study the effect of TiO2 nanoparticles combination with antibiotics and plant extracts against some Gram negative bacteria. Baghdad science journal, 13(3).
8. Al-Zubaidi, M.J. (2021). Molecular characterization of some serotypes ofDiarrheagenic Escherichiacoli bacteria causing diarrhea in Najaf Governorate. MSc Thesis . Education for Girls/University of Kufaas.
9. Berthe T , Ratajczak M , Clermont O , Denamur E , Petit F .( 2013). Evidence for coexistence of distinct Escherichia coli populations in various aquatic environments and their survival in estuary water. Appl Environ Microbiol;79:4684–93
10. Clermont O , Christenson JK , Denamur E . (2013).The Clermont Escherichia coli phylotyping method revisited: improvement of specificity and detection of new phylogroups. Environ Microbiol Rep.5:58–65 .
11. Clermont, O., Lescat, M., O’Brien, C.L., Gordon, D.M., Tenaillon, O., and Denamur, E. (2008) Evidence for a humanspecific Escherichia coli clone. Environ Microbiol 10: 1000–10006.
12. Clermont, O., Bonacorsi, S. and Bingen, E., (2000). Rapid and simple determination of the Escherichia coli phylogenetic group. Applied and environmental microbiology, 66(10), pp.4555-4558.
13. Clermont, O., Bonacorsi, S., and Bingen, E. (2004) Characterization of an anonymous molecular marker stronglylinked to Escherichia coli strains causing neonatal meningitis. J Clin Microbiol 42: 1770–1772.
14. Emami, A., Pirbonyeh, N., Javanmardi, F., Bazargani, A., Moattari, A., Keshavarzi, A. and Akbari, A.,( 2021). Molecular diversity survey on iarrheagenic Escherichia coli isolates among children with gastroenteritis in Fars, Iran. Future Microbiology, 16(17), pp.1309-1318.
15. Rezouqi,N.S. ; Ramadhan, A.; Nasir , B.; Hasin,W.A. (2016). A Study of the Effect of Some Growth Conditions on Inhibitory Activity of Lactobacillus delbrueckii and Lactobacillus fermentum to reduce Growth of E. coli. Iraqi Journal of Science. Vol. 57.(2) .1357-1364.
16. Galal ,S. (2021).Total population of South Africa 2019, by province. Available at: https://www.statista.com/statistics/1112169/ total-opulation- of- south- africa- by- province.
17. Iranpour, D , Hassanpour M , Ansari M , Tajbakhsh S , Khamisipour G , NajafiA . (2015). Phylo- genetic groups of Escherichia coli strains from patients with urinary tract infec- tion in Iran based on the new Clermont phylotyping method.BioMed Res Int;846219. J Clin Microbiol 42:1770–1772.
18. Javadi, K., Mohebi, S., Motamedifar, M. and Hadi, N., (2020). Characterization and antibiotic resistance pattern of diffusely adherent Escherichia coli (DAEC), isolated from paediatric diarrhoea in Shiraz, southern Iran. New Microbes and New Infections, 38, p.100780.)
19. Katongole, P , Kisawuzi DB , Bbosa HK , Kateete DP , Najjuka CF . (2019).Phylogenetic groups and antimicrobial susceptibility patterns of uropathogenic Escherichia coli clin- ical isolates from patients at Mulago National Referral Hospital, Kampala, Uganda. F10 0 0Research;8:1828.
20. Khairy, R.M., Fathy, Z.A., Mahrous, D.M., Mohamed, E.S. and Abdelrahim, S.S., (2020). Prevalence, phylogeny, and antimicrobial resistance of Escherichia coli pathotypes isolated from children less than 5 years old with community acquired-diarrhea in Upper Egypt. BMC Infectious Diseases, 20(1), pp.1-9.
21. Lescat, M., Clermont, O., Woerther, P.L., Glodt, J., Dion, S., Skurnik, D., et al. (2012) Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with hostdependant population structure. Environ Microbiol Rep.DOI: 10.1111/j.1758-2229.2012.00374.x
22. .Omolajaiye, S. A., Afolabi, K. O., and Iweriebor, B. C. (2020). Pathotyping and Antibiotic Resistance Profiling of Escherichia coli Isolates from Children with Acute Diarrhea in Amatole District Municipality of Eastern Cape , South Africa. BioMed Research International.
23. Park, S.B.; Park, Y.K.; Ha, M.W.; Thompson, K.D.; Jung, T.S.(2022)..Antimicrobial Resistance, Pathogenic,and Molecular Characterization of Escherichia coli from Diarrheal Patients in South Korea. Pathogens , 11, 385. https://doi.org/10.3390/pathogens11040385
24. Ramírez-Larrota, J. S., & Eckhard, U. (2022). An Introduction to bacterial biofilms and their proteases, and their roles in host infection and immune evasion. Biomolecules, 12(2), 306.
25. Snehaa, K., Singh, T., Dar, S.A., Haque, S., Ramachandran, V.G., Saha, R., Shah, D. and Das, S., 2021. Typical and atypical enteropathogenic Escherichia coli in children with acute diarrhoea: changing trend in East Delhi. biomedical journal, 44(4), pp.471-478.
26. Hassan, D.F. and Mahmood, M.B., (2019). Biosynthesis of iron oxide nanoparticles using Escherichia coli. Iraqi Journal of Science, pp.453-459.
27. Ghafil, J.A., ( 2018). Assessment the effect of non-thermal plasma on Escherichia coli and Staphylococcus aureus biofilm formtion in vitro. Iraqi Journal of Science, pp.25-29.