The Impressive Evaluation of Selenium Yeast Role Against Diabetes Mellitus and its Comorbidities by Streptozotocin-Induced Diabetes in Rats

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Lina N Tamimi
Zainab Zakaraya
Wael Abu Dayyih
Thana Y. ALjaraedah
Rima H. Al Omari
Mohammad Abu Assab
Mohamed J. Saadh
Basim Mareekh
Mohammed F. Hamad


Selenium yeast; Anti-diabetic; Antioxidant; Anti-inflammatory; Diabetes mellitus


Current anti-diabetic drugs exhibit many side-effects and alternative strategies with minimal side effects and low cost are greatly needed. This study aimed to evaluate selenium yeast (SY) role against diabetes mellitus (DM) in Streptozotocin (STZ)-induced DM. STZ was intraperitoneal injected daily to Sprague-dawley rats to induce DM. SY (0.2 mg/kg) anti-diabetic effect on DM complications was evaluated after 8 weeks of treatment. Biochemical analyses were performed to evaluate SY effectiveness on glucose level, insulin sensitivity, lipid disturbances, oxidative mediators and inflammatory markers. STZ induced DM with toxic effect accompanied with toxic hepatic tissues, lipid disturbances, remarkable oxidative damage and hyper-inflammation. Although there was no significant difference, SY anti-diabetic effect illustrated by decreases in fasting blood glucose, insulin, HbA1c and HOMA-IR levels. SY significantly attenuated (P<0.05) lipid disturbances and their associated elevated atherogenic biomarkers. Moreover, SY treatments exhibited an anti-inflammatory effect as it ameliorated the increase in inflammatory parameters (CRP, TNF-α, IL-6). Also, it restored total antioxidant capacity and peroxisome proliferator-activated receptor (PPARƔ) levels that decreased by STZ-DM induction. This study highlighted SY promising therapeutic role in DM. SY ameliorated lipid accumulation, alleviated pro-inflammatory cytokines expression and restoring the antioxidant capacity. Further studies needed to evaluate SY combination with other standard anti-diabetic drugs to improve its efficacy.

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1. Hassan, R.M., et al., Mitigating effect of single or combined administration of nanoparticles of zinc oxide, chromium oxide, and selenium on genotoxicity and metabolic insult in fructose/streptozotocin diabetic rat model. Environ Sci Pollut Res Int, 2021. 28(35): p. 48517-48534.
2. Singh, P., et al., Assessment of antidiabetic effect of 4-HIL in type 2 diabetic and healthy Sprague Dawley rats. Hum Exp Toxicol, 2022. 41: p. 9603271211061873.
3. Nasir, A., et al., Anti-diabetic potential of ‘Safūf-i-Dhayābītus’ as standalone and as an adjuvant with glibenclamide in streptozotocin-induced diabetic rats. Phytomedicine Plus, 2022. 2(1): p. 100218.
4. Hailat, M.; Zakaraya, Z.;Al-Ani, I.; Meanazel, O.A.;Al-Shdefat, R.; Anwer, M.K.; Saadh,M.J.; Abu Dayyih,W. Pharmacokinetics and Bioequivalence of Two Empagliflozin,with Evaluation in Healthy Jordanian Subjects under Fasting and Fed Conditions. Pharmaceuticals 2022, 15,193.
5. Amawi, Kawther Faisal, et al., Diabetes upregulates the expression of HSP90 and downregulates HSP70 in the liver of diabetic rats. Comparative Clinical Pathology, 2019. 28: p. 473-478.
6. Kim, Y.D., et al., Metformin inhibits hepatic gluconeogenesis through AMP-activated protein kinase-dependent regulation of the orphan nuclear receptor SHP. Diabetes, 2008. 57(2): p. 306-14.
7. Aziz, T.A., et al., The efficacy and safety of Ginkgo biloba extract as an adjuvant in type 2 diabetes mellitus patients ineffectively managed with metformin: a double-blind, randomized, placebo-controlled trial. Drug Des Devel Ther, 2018. 12: p. 735-742.
8. L.N. Tamimi, Z. Zakaraya, M. Hailat, W. Abu Dayyih, E. Daoud, A. Abed, M.J. Saadh, et al. Anti-diabetic effect of cotreatment with resveratrol and pioglitazone in diabetic rats. European Review for Medical and Pharmacological Sciences, 2023; 27: 325-332
9. Steinbrenner, H., L.H. Duntas, and M.P. Rayman, The role of selenium in type-2 diabetes mellitus and its metabolic comorbidities. Redox Biol, 2022. 50: p. 102236.
10. Tanko, Y., et al., Effects of Selenium Yeast on Blood Glucose and Antioxidant Biomarkers in Cholesterol Fed Diet Induced Type 2 Diabetes Mellitus in Wistar Rats. Nig J Physiol Sci, 2017. 31: p. 147-152.
11. National Research Council, Guide for the Care and Use of Laboratory Animals. 8th edition ed. 2011: Washington (DC): National Academies Press (US).
12. Wareham, N.J., et al., The 30 minute insulin incremental response in an oral glucose tolerance test as a measure of insulin secretion. Diabet Med, 1995. 12(10): p. 931.
13. Alkather, Zainab; Hailat, Mohammad; Al-Shdefat, Ramadan; Abu Dayyih, Wael. Development and Validation of HPLC Method for Five Gliptins in Pharmaceutical Dosage Forms in Finished Marketed Products. Current Pharmaceutical Analysis, 2021, 17( 10), pp. 1263-1271(9
14. Steinbrenner, H., Interference of selenium and selenoproteins with the insulin-regulated carbohydrate and lipid metabolism. Free Radic Biol Med, 2013. 65: p. 1538-1547. 15. Hariharan, S. and S. Dharmaraj, Selenium and selenoproteins: it's role in regulation of inflammation. Inflammopharmacology, 2020. 28(3): p. 667-695.
16. Abdulmalek, S.A. and M. Balbaa, Synergistic effect of nano-selenium and metformin on type 2 diabetic rat model: Diabetic complications alleviation through insulin sensitivity, oxidative mediators and inflammatory markers. PLoS One, 2019. 14(8): p. e0220779.
17. Ahmed, M.K., et al., Ameliorative Effect of Selenium Yeast on Blood Glucose Level in Streptozotocin Induced Diabetes in Wistar Rats. Cell Biology, 2015. 3(1): p. 14-18.
18. Abd El-Hakim, Y.M., et al., Chitosan-Stabilized Selenium Nanoparticles and Metformin Synergistically Rescue Testicular Oxidative Damage and Steroidogenesis-Related Genes Dysregulation in High-Fat Diet/Streptozotocin-Induced Diabetic Rats. Antioxidants (Basel), 2020. 10(1).
19. Zainab ZZ et al. Ameliorative effect of selenium yeast in combination with pioglitazone on diabetes outcomes in streptozotocin-induced J Popul Ther Clin Pharmacol, December 2022.Vol 29(4):e202–e210; 24
20. Steinbrenner, H., et al., High selenium intake and increased diabetes risk: experimental evidence
for interplay between selenium and carbohydrate metabolism. J Clin Biochem Nutr, 2011. 48(1): p. 40-5.
21. Hei, Y.J., et al., Stimulation of MAP kinase and S6 kinase by vanadium and selenium in rat adipocytes. Mol Cell Biochem, 1998. 178(1-2): p. 367-75.
22. Ezaki, O., The insulin-like effects of selenate in rat adipocytes. J Biol Chem, 1990. 265(2): p. 1124-8.
23. Agbor, G.A., et al., Effect of selenium- and glutathione-enriched yeast supplementation on a combined atherosclerosis and diabetes hamster model. J Agric Food Chem, 2007. 55(21): p. 8731-6.
24. AL-Auqbi, T.F.R., A.M.R. Al-Mussawi, and A.-S.A.K.Y. J., Evaluation of the Potential Role of Serum Selenium in Diabetic Patients. AL-Kindy College Medical Journal, 2008. 4(2): p. 45-49.
25. Matough, F.A., et al., The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J, 2012. 12(1): p. 5-18.
26. Talbi, W., et al., Effects of selenium on oxidative damage and antioxidant enzymes of eukaryotic cells: wine Saccharomyces cerevisiae. J Appl Microbiol, 2019. 126(2): p. 555-566.

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