Protective Action of SIRT1 Activator Aptamer in Human Skin Cell Line
Main Article Content
Keywords
Reactive oxygen species, Human keratinocytes cell line (HaCaT), SIRT1 aptamer, Apoptosis
Abstract
The skin is the largest organ of the body. The general aging process, which is genetically fixed and happens solely with the passage of time, is referred to as the intrinsic skin aging process. The skin aging process caused by external causes is referred to as extrinsic skin aging. The skin must endure a continual bombardment from the outside by reactive oxygen species, or ROS, which are given to the skin by the environment and created within the skin itself, either as a reaction to incoming UV radiation or made when mitochondria aerobic respiration. An increase in the levels of ROS lead to damage the mtDNA, affecting cell signaling and inducing the apoptosis responses, such as senescence, fibrosis, calcification, and hypertrophy. During the past decade, investigators have reported the relationship between disturbance of SIRT1 activation and the onset of aging. Sirtuin1 is indispensable for DNA repair which make it good anti-senescence/anti-ageing targets and because ROS and SIRT1 are disturbed in the aging process. Because of its enhancing effect on reactive oxygen species and apoptotic pathways, an aberrant increase in NO generation has been linked to early skin aging. We'll look at how SIRT1 aptamer (as a SIRT1 activator) can protect cells against sodium nitroprusside-induced cell death (SNP), employing a human keratinocyte cell line to study a well-known NO generating chemical with suspected harmful and apoptotic effects on keratinocytes (HaCaT). As a result, the primary goal of this research is to discover and define the protective effects of SIRT1 activators in human skin cells. Finally, the findings imply that SIRT1 aptamer might be effective in preventing skin aging caused by reactive oxygen species (ROS).
References
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33. Wlodkowic D, Skommer J, Pelkonen J. Towards an understanding of apoptosis detection by SYTO dyes. Cytometry Part A: The Journal of the International Society for Analytical Cytology. (2007); 71(2): 61-72.
34. Chung K. W., Choi Y. J., Park M. H., Jang E. J., Kim D. H., Park B. H. Molecular insights into SIRT1 protection against UVB-induced skin fibroblast senescence by suppression of oxidative stress and p53 acetylation. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, (2015). 70.8; 959:968.
35. Takayama K, Ishida K, Matsushita T, Fujita N, Hayashi S, Sasaki K, Kuroda R. SIRT1 regulation of apoptosis of human chondrocytes. Arthritis & Rheumatism, (2009).60(9); 2731:2740.
36. Reichert, A. S,Neupert W. Contact sites between the outer and inner membrane of mitochondria—role in protein transport. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. (2002); 1592(1): 41-49.
37. Horvath S. E., Daum G. Lipids of mitochondria. Progress in lipid research. (2013); 52(4): 590-614.
38. Unsay J. D., Cosentino K, Subburaj Y., García-SáezA. J. Cardiolipin effects on membrane structure and dynamics. Langmuir. (2013); 29(51): 15878-15887.
39. Bernardi, P. The permeability transition pore. Control points of a cyclosporin A-sensitive mitochondrial channel involved in cell death. Biochimica et Biophysica Acta (BBA)-Bioenergetics. (1996); 1275(1-2):5-9.
40. Zamzami N, Maisse C, Métivier D, Kroemer G. Measurement of membrane permeability and the permeability transition of mitochondria. Methods in cell biology. (2007); 80: 327-340.
41. Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper J. M. Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry. Experimental cell research. (2009); 315(10): 1706-1714.
42. Kaštelan M, Prpić-Massari L.,Brajac I. Apoptosis in psoriasis. Acta Dermatovenerologica Croatica. (2009); 17(3): 0-0.
43. Briganti S, Picardo, M. Antioxidant activity, lipid peroxidation and skin diseases. What's new. Journal of the European Academy of Dermatology and Venereology. (2003);17(6): 663-669.
44. Kamata H, Honda S. I., Maeda S, Chang L, Hirata H, Karin M. Reactive oxygen species promote TNFα-induced death and sustained JNK activation by inhibiting MAP kinase phosphatases. (2005). 120(5):649-661.
45. Xia Y., Dawson V. L., Dawson T. M., Snyder S. H., Zweier J. L.Nitric oxide synthase generates
superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proceedings of the National Academy of Sciences. (1996); 93(13): 6770-6774.
46. Kobayashi M, Shu S, Marunaka K, Matsunaga T, Ikari A. Weak ultraviolet B enhances the mislocalization of claudin-1 mediated by nitric oxide and peroxynitrite production in human keratinocyte-derived HaCaT cells. International journal of molecular sciences. 2020 Jan;21(19):7138.
47. Tejero J, Shiva S, Gladwin M. T. Sources of vascular nitric oxide and reactive oxygen species and their regulation. Physiological reviews. (2019); 99(1): 311-379.
48. Cals-Grierson M. M, Ormerod A. D. Nitric oxide function in the skin. Nitric oxide. (2004);10(4): 179-193.
49. Dawson V. L., Dawson T. M., London E. D., Bredt, D. S., Snyder S. H. Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures. Proceedings of the National Academy of sciences. (1991); 88(14): 6368-6371.
50. Bastianetto S., Zheng W. H., Quirion, R. Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide‐related toxicity in cultured hippocampal neurons. British journal of pharmacology. (2000); 131(4): 711-720.
51. Wu S., Wang L., Jacoby A. M., Jasinski K., Kubant R., Malinski, T. Ultraviolet B Light‐induced Nitric Oxide/Peroxynitrite Imbalance in Keratinocytes—Implications for Apoptosis and Necrosis. Photochemistry and photobiology. (2010); 86(2): 389-396.
52. Pérez-Matute P., Zulet M. A., Martínez J. A. Reactive species and diabetes: counteracting oxidative stress to improve health. Current opinion in pharmacology. (2009); 9(6): 771-779.
53. Zhang L., Lei J., Liu J., Ma F., Ju H. In situ activation and monitoring of the evolution of the intracellular caspase family. Chemical science. (2015); 6(6): 3365-3372.
54. Takayama K., Ishida K., Matsushita T., Fujita N., Hayashi S, Sasaki K, Kuroda, R. SIRT1 regulation of apoptosis of human chondrocytes. Arthritis & Rheumatism. (2009); 60(9): 2731-2740.
55. Wu Y., Zhao D., Zhuang, J., Zhang F., Xu C. Caspase-8 and caspase-9 functioned differently at different stages of the cyclic stretch-induced apoptosis in human periodontal ligament cells. PLoS One. (2016);11(12): e0168268.
2. Cabral-Pacheco GA, Garza-Veloz I, Castruita-De la Rosa C, Ramirez-Acuña JM, Perez-Romero BA, Guerrero-Rodriguez JF, et al.The Roles of Matrix Metalloproteinases and Their Inhibitors in Human Diseases. 2020.Dec 20;21(24):9739.
3. Calapre L., Gray E. S., Kurdykowski S., David, A. Descargues, P., Ziman, M. SIRT1 activation mediates heat-inuced survival of UVB damaged Keratinocytes. BMC dermatology. (2017). 17(1): 8.
4. Ming M., Soltani K., Shea C. R., Li, X., & He Y. Y. Dual role of SIRT1 in UVB-induced skin tumorigenesis. Oncogene. 2015 Jan 15;34(3):357-63.
5. Al-Sudani B, Ragazzon-Smith AH, Aziz A, Alansari R, Ferry N, Krstic-Demonacos M, Ragazzon PA. Circular and linear: a tale of aptamer selection for the activation of SIRT1 to induce death in cancer cells. RSC Advances. 2020;10(73):45008-18.
6. Bielach-Bazyluk A., Zbroch E., Mysliwiec H., Rydzewska-Rosolowska A., Kakareko K., Flisiak I ,et al, Sirtuin 1 and skin: implications in intrinsic and extrinsic aging—a systematic review. Cells. (2021). 10(4): 813.
7. Al-Sudani BT, Hameed B, Alahmar AT. Gene Expression Analysis in MOTN-1 Cell Line after Treating with New Development Aptamer. blood cells. 2020 Feb 1;4:5.
8. Joudah MS, Al-Sudani BT, Arif IS. New Bio-Therapeutic Candidate for Pancreatic Cancer. Indian Journal of Forensic Medicine & Toxicology. 2020 Jan 1;14(1).
9. Talib Lj, Al-Abbassi Mg, Al-Sudani BT. Effect of SIRT1 Activators on Human Follicular Thyroid Cancer. International Journal of Pharmaceutical Research. 2020 Oct;12(4).
10. Qusay A, Marie NK, Al-Sudani BT. Utilization of natural stabilizer to prepare liposomal conjugate for the newly developed aptamer. Systematic Reviews in Pharmacy. 2020;11(7):32-50.
11. TARIQ RA, Al-Sudani BT, Arif IS. Promising Preclinical Data to a Possible Antidiabetic Drug International Journal of Pharmaceutical Research, 2020,12 (4).
12. Joudah MS, Arif IS, Al-Sudani BT. Crosstalk Between Sirt1 Activators And Nf-Κb Axis As A Therapeutic Target To Reduce Pancreatic Cancer. Systematic Reviews in Pharmacy. 2021;12(3):207-12.
13. Al-Sudani BT. Novel therapies for cancer treatment: designing high affinity selective ligands against SIRT1 enzyme. University of Salford (United Kingdom); 2017.
14. Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, Minor L. Cell viability assays. Assay Guidance Manual [Internet]. 2016 Jul 1.
15. Neri S, Mariani E, Meneghetti A, Cattini L, Facchini A. Calcein-acetyoxymethyl cytotoxicity assay: standardization of a method allowing additional analyses on recovered effector cells and supernatants. Clinical Diagnostic Laboratory Immunology. 2001 Nov 1;8(6):1131-5.
16. Wlodkowic D, Skommer J, Darzynkiewicz Z. Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes. InMammalian Cell Viability 2011 (pp. 81-89).
17. Elefantova K, Lakatos B, Kubickova J, Sulova Z, Breier A. Detection of the mitochondrial membrane potential by the cationic dye JC-1 in L1210 cells with massive overexpression of the plasma membrane ABCB1 drug transporter. International journal of molecular sciences. 2018 Jul;19(7):1985.
18. Lee H, Lee D, Kang KS, Song JH, Choi YK. Inhibition of intracellular ROS accumulation by formononetin attenuates cisplatin-mediated apoptosis in LLC-PK1 cells. International journal of molecular sciences. 2018 Mar;19(3):813.
19. Kumar S, Kashyap P. Antiproliferative activity and nitric oxide production of a methanolic extract of Fraxinus micrantha on Michigan Cancer Foundation-7 mammalian breast carcinoma cell line. Journal of intercultural ethnopharmacology. 2015 Apr;4(2):109.
20. El-Huneidi W, Shehab NG, Bajbouj K, Vinod A, El-Serafi A, Shafarin J, Bou Malhab LJ, Abdel-Rahman WM, Abu-Gharbieh E. Micromeria fruticosa induces cell cycle arrest and apoptosis in breast and colorectal cancer cells. Pharmaceuticals. 2020 Jun;13(6):115.
21. Weller R. Nitric oxide: a key mediator in cutaneous physiology. Clin Exp Dermatol. (2003). 28: 511–514. 1365.
22. Xia Y, Dawson VL, Dawson TM, Snyder SH, Zweier JL. Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proc Natl Acad Sci U S A. (1996). 93: 6770–6774.
23. Bastianetto S, Dumont Y, Duranton A, Vercauteren F, Breton L, Quirion R. Protective action of resveratrol in human skin: possible involvement of specific receptor binding sites. Plos one. 2010; 5.9: e12935.
24. Serafeim A, HolderM. J, Grafton G, Chamba A, Drayson M. T, Luong Q. T, et al. Selective serotonin reuptake inhibitors directly signal for apoptosis in biopsy-like Burkitt lymphoma cells. Blood, The Journal of the American Society of Hematology. 2003;101.8: 3212-3219.
25. Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J. Novel fluorescence assay using calcein‐AM for the determination of human erythrocyte viability and aging. Cytometry Part A: the journal of the International Society for Analytical Cytology. 2005; 66.1: 78-84.
26. Probes M. Molecular SYTO green-fluorescent nucleic acid stains. Oregon, USA Molecular Probes, Eugene. 2003.
27. Wlodkowic D, Skommer J., Faley S, Darzynkiewicz Z, Cooper J. M. Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry. Experimental cell research.2009; 315.10: 1706-1714.
28. Kaštelan M., Prpić-Massari L., Brajac, I. Apoptosis in psoriasis. Acta Dermatovenerologica Croatica. (2009); 17(3): 0-0.
29. Kuranaga, E. Beyond apoptosis: caspase regulatory mechanisms and functions in vivo. Genes to Cells. (2012); 17(2): 83-97.
30. El‐Domyati M, Moftah N. H, Nasif G. A., Abdel‐Wahab H. M., Barakat, M. T, Abdel‐Aziz, R. T. Evaluation of apoptosis regulatory proteins in response to PUVA therapy for psoriasis. Photodermatology, photoimmunology photomedicine. (2013); 29(1): 18-26.
31. Yao X, Hao S, Yu P. Association study of the caspase gene family and psoriasis vulgaris susceptibility in northeastern China. BioMed research international. 2019.
32. Kim, E., Han, S. Y., Hwang, K, Kim, D, Kim, E. M, Hossain, M. A, Cho, J. Y. Antioxidant and cytoprotective effects of (−)-epigallocatechin-3-(3 ″-o-methyl) gallate. International journal of molecular sciences. (2019); 20(16): 3993.
33. Wlodkowic D, Skommer J, Pelkonen J. Towards an understanding of apoptosis detection by SYTO dyes. Cytometry Part A: The Journal of the International Society for Analytical Cytology. (2007); 71(2): 61-72.
34. Chung K. W., Choi Y. J., Park M. H., Jang E. J., Kim D. H., Park B. H. Molecular insights into SIRT1 protection against UVB-induced skin fibroblast senescence by suppression of oxidative stress and p53 acetylation. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, (2015). 70.8; 959:968.
35. Takayama K, Ishida K, Matsushita T, Fujita N, Hayashi S, Sasaki K, Kuroda R. SIRT1 regulation of apoptosis of human chondrocytes. Arthritis & Rheumatism, (2009).60(9); 2731:2740.
36. Reichert, A. S,Neupert W. Contact sites between the outer and inner membrane of mitochondria—role in protein transport. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. (2002); 1592(1): 41-49.
37. Horvath S. E., Daum G. Lipids of mitochondria. Progress in lipid research. (2013); 52(4): 590-614.
38. Unsay J. D., Cosentino K, Subburaj Y., García-SáezA. J. Cardiolipin effects on membrane structure and dynamics. Langmuir. (2013); 29(51): 15878-15887.
39. Bernardi, P. The permeability transition pore. Control points of a cyclosporin A-sensitive mitochondrial channel involved in cell death. Biochimica et Biophysica Acta (BBA)-Bioenergetics. (1996); 1275(1-2):5-9.
40. Zamzami N, Maisse C, Métivier D, Kroemer G. Measurement of membrane permeability and the permeability transition of mitochondria. Methods in cell biology. (2007); 80: 327-340.
41. Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper J. M. Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry. Experimental cell research. (2009); 315(10): 1706-1714.
42. Kaštelan M, Prpić-Massari L.,Brajac I. Apoptosis in psoriasis. Acta Dermatovenerologica Croatica. (2009); 17(3): 0-0.
43. Briganti S, Picardo, M. Antioxidant activity, lipid peroxidation and skin diseases. What's new. Journal of the European Academy of Dermatology and Venereology. (2003);17(6): 663-669.
44. Kamata H, Honda S. I., Maeda S, Chang L, Hirata H, Karin M. Reactive oxygen species promote TNFα-induced death and sustained JNK activation by inhibiting MAP kinase phosphatases. (2005). 120(5):649-661.
45. Xia Y., Dawson V. L., Dawson T. M., Snyder S. H., Zweier J. L.Nitric oxide synthase generates
superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proceedings of the National Academy of Sciences. (1996); 93(13): 6770-6774.
46. Kobayashi M, Shu S, Marunaka K, Matsunaga T, Ikari A. Weak ultraviolet B enhances the mislocalization of claudin-1 mediated by nitric oxide and peroxynitrite production in human keratinocyte-derived HaCaT cells. International journal of molecular sciences. 2020 Jan;21(19):7138.
47. Tejero J, Shiva S, Gladwin M. T. Sources of vascular nitric oxide and reactive oxygen species and their regulation. Physiological reviews. (2019); 99(1): 311-379.
48. Cals-Grierson M. M, Ormerod A. D. Nitric oxide function in the skin. Nitric oxide. (2004);10(4): 179-193.
49. Dawson V. L., Dawson T. M., London E. D., Bredt, D. S., Snyder S. H. Nitric oxide mediates glutamate neurotoxicity in primary cortical cultures. Proceedings of the National Academy of sciences. (1991); 88(14): 6368-6371.
50. Bastianetto S., Zheng W. H., Quirion, R. Neuroprotective abilities of resveratrol and other red wine constituents against nitric oxide‐related toxicity in cultured hippocampal neurons. British journal of pharmacology. (2000); 131(4): 711-720.
51. Wu S., Wang L., Jacoby A. M., Jasinski K., Kubant R., Malinski, T. Ultraviolet B Light‐induced Nitric Oxide/Peroxynitrite Imbalance in Keratinocytes—Implications for Apoptosis and Necrosis. Photochemistry and photobiology. (2010); 86(2): 389-396.
52. Pérez-Matute P., Zulet M. A., Martínez J. A. Reactive species and diabetes: counteracting oxidative stress to improve health. Current opinion in pharmacology. (2009); 9(6): 771-779.
53. Zhang L., Lei J., Liu J., Ma F., Ju H. In situ activation and monitoring of the evolution of the intracellular caspase family. Chemical science. (2015); 6(6): 3365-3372.
54. Takayama K., Ishida K., Matsushita T., Fujita N., Hayashi S, Sasaki K, Kuroda, R. SIRT1 regulation of apoptosis of human chondrocytes. Arthritis & Rheumatism. (2009); 60(9): 2731-2740.
55. Wu Y., Zhao D., Zhuang, J., Zhang F., Xu C. Caspase-8 and caspase-9 functioned differently at different stages of the cyclic stretch-induced apoptosis in human periodontal ligament cells. PLoS One. (2016);11(12): e0168268.
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Mar 29, 2023
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Rana Faris Salman, Basma Talib Al-Sudani, & Bahir Abdul Razzaq Mshimesh. (2023). Protective Action of SIRT1 Activator Aptamer in Human Skin Cell Line. Journal of Population Therapeutics and Clinical Pharmacology, 30(5), 342–359. https://doi.org/10.47750/jptcp.2023.30.05.035
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