The Effect of Infectious Bronchitis Virus In Embryonated Chicken Eggs
Main Article Content
Keywords
Infectious bronchitis virus, embryonated chicken eggs, RT-PCR, allantoic sac
Abstract
Specimens were collected from different farms in Iraq, then collected samples from (Baghdad, Babylon, Diyala, Wasit and Sallahulldin) were prepared by mortar and pestle and were inoculated into chicken embryos at age (9-11) days by allantoic sac inoculation. The death of embryos after 24 hr. post inoculation was ignored. There was no death of embryos on the first passage of the virus into chicken embryos and the allantoic fluid was harvested after 96 hours post inoculation. On second passage, one embryo was died and the allantoic fluid was harvested after 96 hr. post inoculation. On third passage, two embryos were died post inoculation and the allantoic fluid was harvested after 96 hr. The infected allantoic fluid was examined by RT-PCR to detect IBV. The result of RT-PCR was positive to detect IBV in infected harvested allantoic fluid.
References
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2. Flageul, A., Allée, C., Courtillon, C., Béven, V., Quenault, H., Blanchard, Y., ... & Brown, P. A. (2022). Infectious Bronchitis Coronavirus: Genome Evolution In Vaccinated And Non-Vaccinated SPF Chickens. Viruses, 14(7), 1392.DOI: Https://Doi.Org/10.3390/V14071392
3. Abbood, S. S., & Ali, B. H. (2022). Molecular Similarly Between Infectious Bronchitis Viruses And Common Vaccines. Revista Iberoamericana De Psicología Del Ejercicio Y El Deporte, 17(5), 311-315.
4. Lin, S. Y., & Chen, H. W. (2017). Infectious Bronchitis Virus Variants: Molecular Analysis And Pathogenicity Investigation. International Journal Of Molecular Sciences, 18(10), 2030.DOI: Https://Doi.Org/10.3390/Ijms18102030
5. AL-Zuhariy, M. T. (2017). Improved Vaccine Strategies Of Infectious Bronchitis Disease To Reduce Shedding Of Virulent Virus From Infected Birds. مجلة الكوفة للعلوم الطبية البيطرية | Kufa Journal For Veterinary Medical Sciences, 8(1).
6. Mahmood, A., Zahid, A., & Al-Khafaji, Z. (2017). In Silico Characterization Of Infectious Bronchitis Coronavirus (Qx Strain) Circulating In Iraq. International Research Journal Of Pharmaceutical Sciences, 7, 1-18.
7. Franzo, G., Massi, P., Tucciarone, C. M., Barbieri, I., Tosi, G., Fiorentini, L., & Moreno, A. (2017). Think Globally, Act Locally: Phylodynamic Reconstruction Of Infectious Bronchitis Virus (IBV) QX Genotype (GI-19 Lineage) Reveals Different Population Dynamics
And Spreading Patterns When Evaluated On Different Epidemiological Scales. Plos One, 12(9), E0184401.DOI: Https://Doi.Org/10.1371/Journal.Pone.0184401
8. Hussein, M. A., Sabbar, A. A., & Khammas, E. J. (2018). Isolation And Identification Of Infectious Bronchitis Virus And Experimental Infection In Broilers. Diyala Agricultural Sciences Journal, 10(Special Issue), 290-302
9. Cabal, A. B. S., & Wu, T. Y. (2022). Recombinant Protein Technology In The Challenging Era Of Coronaviruses. Processes, 10(5), 946.DOI: Https://Doi.Org/10.3390/Pr10050946
10. Isa, R. H., Abdo, J. M., & AL-Barzinji, Y. M. (2022). Genotyping Of Avian Infectious Bronchitis Virus In Broiler Farms In Duhok Province, North Of Iraq. Iraqi Journal Of Veterinary Sciences, 36(1), 171-175.DOI: Https://Doi.Org/10.33899/Ijvs.2021.129635.1670
11. Benyeda Z, Szeredi L, Mato T, Suveges T, Balka G, Abonyitoth Z Et Al (2010). Comparative Histopathology And Immunohistochemistry Of QX-Like, Massachusetts And 793/B Serotypes Of Infectious Bronchitis Virus Infection In Chickens. J Comp Pathol. 143(4):276–283. DOI: Medicine, 32(1), 223-230.DOI: Https://Doi.Org/10.1016/J.Jcpa.2010.04.007
12. Najimudeen, S. M., Barboza-Solis, C., Ali, A., Buharideen, S. M., Isham, I. M., Hassan, M. S., & Abdul-Careem, M. F. (2022). Pathogenesis And Host Responses In Lungs And Kidneys Following Canadian 4/91 Infectious Bronchitis Virus (IBV) Infection In Chickens. Virology, 566, 75-88.DOI: Https://Doi.Org/10.1016/J.Virol.2021.11.013
13. Khamas, E. J. (2008). Avian Influenza (H9N2) Outbreak In Iraq. The Iraqi Journal Of Veterinary Medicine, 32(1), 223-230.DOI: Https://Doi.Org/10.30539/Iraqijvm.V32i1.782
14. Abdulwahid, M. T., Zahid, A. H., & Kadhum, M. J. (2016). Effect Of Vitamin E And Cod Liver Oil Supplement With Bivalent Oil Based Vaccine Of Newcastle Disease And Infectious Bronchitis Disease On Immune Response Of The Broilers. Iraqi J. Of Agric. Sci, 47(3), 892-899.DOI: Https://Doi.Org/10.36103/Ijas.V47i3.582
15. Mohammed, M. H., Hair-Bejo, M., Zahid, A., Alazawy, A., Abdul Ahad, E. A., & Hasoon, M. F. (2013). Adaptation Of Infectious Bronchitis Virus In Primary Cells Of The Chick Embryo Chorioallantoic Membrane. Iraqi Journal Of Veterinary Sciences, 27(1), 33-37.
16. Mohammed, M. H. (2013). Isolation Of Infectious Bronchitis Virus In Primary Cells Of The Chick Embryo Chorioallantoic Membrane: MH Mohammed1@; M. Hair-Bejo1; Abdel Ameer Husain Zahid2; Amer Alazawy3; Emad Adwar Abdul Ahad4 And Mauida F. Hasoon1. The Iraqi Journal Of Veterinary Medicine, 37(1), 109-114.DOI: Https://Doi.Org/10.30539/Iraqijvm.V37i1.342
17. Ali, A., Ojkic, D., Elshafiee, E. A., Shany, S., El-Safty, M. M., Shalaby, A. A., & Abdul-Careem, M. F. (2022). Genotyping And In Silico Analysis Of Delmarva (DMV/1639) Infectious Bronchitis Virus (IBV) Spike 1 (S1) Glycoprotein. Genes, 13(9), 1617. DOI: Https://Doi.Org/10.3390/Genes13091617
18. Ameen, S. M., Adel, A., Selim, A., Magouz, A., Aboelkhair, M., & Bazid, A. H. (2022). A Multiplex Real-Time Reverse Transcription Polymerase Chain Reaction Assay For Differentiation Of Classical And Variant II Strains Of Avian Infectious Bronchitis Virus. Archives Of Virology, 167(12), 2729- 2741.DOI: Https://Doi.Org/10.1007/S00705-022-05603-7
19. Atta, R. N., & Allawe, A. B. (2018). Isolation And Sequencing Of Field Isolates Of Avian Infectious Bronchitis Virus In Iraq. J Entomol Zool Stud, 6, 529-540.
20. Ennaji, Y., Khataby, K., & Ennaji, M. M. (2020). Infectious Bronchitis Virus In Poultry: Molecular Epidemiology And Factors Leading To The Emergence And Reemergence Of Novel Strains Of Infectious Bronchitis Virus. In Emerging And Reemerging Viral Pathogens (Pp. 31-44). Academic Press.DOI: Https://Doi.Org/10.1016/B978-0-12-814966-9.00003-2
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Published
Mar 29, 2023
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How to Cite
Mustafa Hilal Ali, & Aida Bara Allawe. (2023). The Effect of Infectious Bronchitis Virus In Embryonated Chicken Eggs. Journal of Population Therapeutics and Clinical Pharmacology, 30(5), 273–278. https://doi.org/10.47750/jptcp.2023.30.05.027
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