Interleukin-4 (C-589T) and Interleukin-17 (-197 G>A) Gene Polymorphisms in Psoriasis patients in Thi-Qar province south of Iraq

Main Article Content

Salah Mahdi Muttar
Hassan Risan Mubarek
Ahmed Abdulhussein kawin


polymorphisms, Interleukin-4, Interleukin-17, psoriasis, PCR


Psoriasis is the common immune-mediated skin diseases which caused by the interaction between genetic and environmental risk factors. The present study aimed to determine the relationship between IL-17 (-197G>A) and IL-4 (C-589T) polymorphisms and susceptibility to psoriasis. This study included 100 patients and 50 healthy. 2-3 ml of Blood samples were collected in EDTA tube and
preserved at -20 temperature for DNA extraction. IL-4 and IL-17 were amplified by Polymerase Chain Reaction (PCR) technique. In this study, 45% of psoriasis patients and 50% of healthy possessed GG genotype of IL-17 (-197 G>A) (wild genotype). 35% of patients and 30% of healthy have GA genotypes with non-significant differences compared to wild genotype (OR=0.77, 95% CI= 0.35- 1.67). The same ratio of patients and healthy (20%) have homozygous AA genotype with nonsignificant differences compared to GG genotype (OR=0.90, 95% CI= 0.36-2.22). As for the IL-4, the CT and TT genotypes showed a higher frequency in the healthy group (36% and 20%, respectively) (OR=2.38, 95% CI= 1.08-5.24) compared to patients’ group (22% and 14% for CT and TT, respectively (OR = 2.07, 95% CI= 0.80-5.34). The T allele frequency was 25% in psoriasis patients and 38% in the healthy group with significant differences (OR = 1.83, 95% CI= 1.09-3.07).

Abstract 182 | pdf Downloads 161


1. Białecka, M., Ostasz, R., Kurzawski, M., Klimowicz, A., Fabianczyk, H., Bojko, P., ... & Drozdzik, M. (2016). IL17A and IL17F gene polymorphism association with psoriasis risk and response to treatment in a polish population. Dermatology, 232(5), 592-596.
2. Carvalho, A. L., & Hedrich, C. M. (2021). The molecular pathophysiology of psoriatic arthritis the complex interplay between genetic
predisposition, epigenetics factors, and the microbiome. Frontiers in molecular biosciences, 8, 662047.
3. Chamoun, M. N., Blumenthal, A., Sullivan, M. J., Schembri, M. A., & Ulett, G. C. (2018). Bacterial pathogenesis and interleukin-17: interconnecting mechanisms of immune regulation, host genetics, and microbial virulence that influence severity of infection. Critical reviews in microbiology, 44(4), 465-486.
4. Coimbra, S., Oliveira, H., Reis, F., Belo, L., Rocha, S., Quintanilha, A., & Santos‐Silva, A. (2010). Interleukin (IL)‐22, IL‐17, IL‐23, IL‐8, vascular endothelial growth factor and tumour necrosis factor‐α levels in patients with psoriasis before, during and after psoralen–ultraviolet A
and narrowband ultraviolet B therapy. British Journal of Dermatology, 163(6), 1282-1290.
5. Craven, N. M., Jackson, C. W., Kirby, B., Perrey, C., Pravica, V., Hutchinson, I. V., & Griffiths, C. E. M. (2001). Cytokine gene polymorphisms in
psoriasis. British Journal of Dermatology, 144(4), 849-853.
6. Dopytalska, K., Ciechanowicz, P., Wiszniewski, K., Szymańska, E., & Walecka, I. (2021). The role of epigenetic factors in psoriasis.
International Journal of Molecular Sciences, 22(17), 9294.
7. Hahn, M., & Ghoreschi, K. (2017). The role of IL-4 in psoriasis. Expert Review of Clinical Immunology, 13(3), 171-173.
8. Hamza, A., Elwafa, R. A. A., & Omar, S. S. (2021). IL17A (rs2275913 G> A) and IL17F (rs2397084 T> C) Gene Polymorphisms: Relation to Psoriasis Risk and Response to Methotrexate.
9. Huang, T. H., Lin, C. F., Alalaiwe, A., Yang, S. C., & Fang, J. Y. (2019). Apoptotic or antiproliferative activity of natural products
against keratinocytes for the treatment of psoriasis. International Journal of Molecular Sciences, 20(10), 2558.
10. Indhumathi, S., Rajappa, M., Chandrashekar, L., Ananthanarayanan, P. H., Thappa, D. M., & Negi, V. S. (2017). T helper-2 cytokine/regulatory Tcell gene polymorphisms and their relation with risk of psoriasis in a South Indian Tamil cohort. Human Immunology, 78(2), 209-215.
11. Kamiya, K., Kishimoto, M., Sugai, J., Komine, M., & Ohtsuki, M. (2019). Risk factors for the development of psoriasis. International Journal of
Molecular Sciences, 20(18), 4347.
12. Kim, S. Y., Hur, M. S., Choi, B. G., Kim, M. J., Lee, Y. W., Choe, Y. B., & Ahn, K. J. (2017). A preliminary study of new single polymorphisms
in the T helper type 17 pathway for psoriasis in the Korean population. Clinical & Experimental Immunology, 187(2), 251-258.
13. Kimmel, G. W., & Lebwohl, M. (2018). Psoriasis: overview and diagnosis. Evidence-based psoriasis, 1-16.
14. Li, Y., Wei, C., Xu, H., Jia, J., Wei, Z., Guo, R., & Gao, X. (2018). The immunoregulation of Th17 in host against intracellular bacterial infection. Mediators of inflammation, 2018.
15. Lonnberg, A. S., Skov, L., Skytthe, A., Kyvik, K., Pedersen, O., & Thomsen, S. (2013). Heritability of psoriasis in a large twin sample. British Journal of Dermatology, 169(2), 412-416.
16. Lowes, M. (2014). Suarez-Fari nas M, Krueger JG. Immunology of Psoriasis. Annu Rev Immunol, 32, 227-255.
17. Mills, K. H. (2022). IL-17 and IL-17-producing cells in protection versus pathology. Nature Reviews Immunology, 1-17.
18. Mohamed, N. S., Siddig, E. E., Ahmed, A. E., Albsheer, M., Abdelbagi, H., Ali, E. T., ... & , R. A. (2020). Frequency distribution of IL17A G197A (rs2275913) and IL-17F A7488G (rs763780).
19. NPF (The National Psoriasis Foundation)(2017). Get the Facts about Psoriasis and Psoriatic Arthritis.
20. Oka, A., Mabuchi, T., Ozawa, A., & Inoko, H. (2012). Current understanding of human genetics and genetic analysis of psoriasis. The Journal of dermatology, 39(3), 231-241.
21. Sanad, E. M., Nazmy, N. N., Abd‐El Hamid El Sayed, R., & Hamed, A. M. (2022). Interleukin‐17A gene single nucleotide polymorphism and its
relation to fungal growth in psoriatic patients: A preliminary study. Journal of Cosmetic Dermatology, 21(7), 3059-3067.
22. Smolnikova, M. V., Freidin, M. B., Barilo, A. A., & Smirnova, S. V. (2019). Analysis of association between cytokine gene polymorphisms and
psoriatic disease in Russians of East Siberia.
23. Springate, D. A., Parisi, R., Kontopantelis, E., Reeves, D., Griffiths, C. E. M., & Ashcroft, D. M. (2017). Incidence, prevalence and mortality of
patients with psoriasis: a UK population‐based cohort study. British Journal of Dermatology, 176(3), 650-658.
24. Tashiro, T., & Sawada, Y. (2022). Psoriasis and Systemic Inflammatory Disorders. International Journal of Molecular Sciences, 23(8), 4457.
25. Zhou, S., & Yao, Z. (2022). Roles of infection in psoriasis. International Journal of Molecular Sciences, 23(13), 6955.