EXTRACTION, EVALUATION, MANUFACTURING, FORMULATION, ANALYSIS, CLINICAL FOR THE DELIVERY OF METABOLIC ENERGY BY LIPOSOMAL TECHNOLOGY USING MEDIUM-CHAIN TRIGLYCERIDE IN THE FORM OF ORAL ENERGY OF SHOTS

Abstract

Medium-chain triglycerides (MCTs) are rapidly metabolized dietary lipids that provide a readily available source of metabolic energy. MCT oil is predominantly composed of caprylic acid (C8) and capric acid (C10), which are rapidly digested, absorbed through the portal circulation, and transported to the liver for β-oxidation, resulting in acetyl-CoA formation, ATP generation, and ketone-body production. Unlike coconut oil, which contains a mixture of fatty acids with a high proportion of lauric acid (C12), MCT oil provides a concentrated source of rapidly metabolized medium-chain fatty acids. The present study was undertaken to develop and evaluate an MCT-based oral energy shot for rapid energy delivery. MCT oil was prepared through raw-material procurement and authentication, preliminary preparation, extraction using the Pre-activated Vedic Methodology for Extraction, filtration, concentration, and standardization. The extracted oil was evaluated for chemical and physicochemical characteristics, phytochemical profile, chromatographic characteristics where applicable, antioxidant activity, microbial quality, and heavy-metal and contamination status. The standardized MCT oil was formulated into an oral shot and evaluated for formulation characteristics, packaging, storage, and stability. The energy value was calculated based on the MCT content of the formulation. The study further considered the complete metabolic pathway of MCTs, including intestinal digestion, portal absorption, hepatic β-oxidation, acetyl-CoA formation, ATP generation, and ketogenesis. A preclinical safety evaluation using guinea pigs was incorporated to assess the tolerability and safety of the developed formulation under defined experimental conditions. Energy values were calculated theoretically from MCT content and were not experimentally measured. Overall, the study integrated MCT production, characterization, metabolic energy delivery, oral-shot formulation, stability assessment, and preclinical safety evaluation to develop a standardized MCT-based functional energy formulation.