Synergistic Activity of Olive Oil and Sesame Oil on The Sensitivity of Aeromonas Hydrophila to Ceftriaxone

Main Article Content

Laith Muslih Najeeb
Yousry Sadoon Rasheed

Keywords

Aeromonas hydrophila, Olive oil, sesame oil, ceftriaxone, Antimicrobial

Abstract

The study was conducted to find out the effect of sesame oil and olive oil in increasing the sensitivity of the local isolate Aeromonas hydrophila to Ceftriaxone (STI 30). The local isolate was taken that causes tooth decay from the laboratories of the College of Science at the University of Anbar, and the initial and confirmatory diagnosis was diagnosed using the VITEK 2, then its sensitivity was tested by the tablet method towards the Ceftriaxone (STI 30), and then its sensitivity was tested against the oils used under study after saturating the empty tablets with these oils, and then the antibiotic activity of these oils was tested with the antibiotic used under study.
The results showed that the isolate is resistant to STI 30, and that the used oils under study had synergistic activity and a significant difference, as the inhibition diameter increased to 43 mm, 14 mm, and 13mm after it was zero when using olive oil, sesame oil, and their mixture, respectively, in synergy with the STI30 used under study

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References

1. Alheety S M S A (2015). Effects of some natural materials on the virulence factors of the pathogenic bacteria isolated from the urinary tract
infection and burn. Master thesis, Department of Biology, College of Education, University of Anbar.
2. Bhowmick U D, Bhattacharjee S (2018). Bacteriological, Clinical and Virulence Aspects of-associated Diseases in Humans. Polish Journal
of Microbiology, 67(2): 137-150.
3. Chen PL, Wu CJ, Chen CS, Tsai PJ, Tang HJ, Ko WC (2014). A comparative study of clinicalAeromonas dhakensis and Aeromonas
hydrophila isolates in southern Taiwan:A. dhakensis is more predominant and virulent. Clin Microbiol Infect, 20:428-434.
4. Elad S, Zadik Y, Caton J G, Epstein J B (2019). Oral mucosal changes associated with primary diseases in other body systems. Periodontology
2000, 80(1): 28-48.
5. Elshinawy M I, Al-Madboly L A, Ghoneim W M, El-Deeb N M (2018). Synergistic effect of newly introduced root canal medicaments; ozonated
olive oil and chitosan nanoparticles, against persistent endodontic pathogens. Frontiers in microbiology, 9: 1371.
6. Hawraa N K A, Hazim A A (2014). Isolation and Characterization of A. hydrophila from the AlJadryia River in Baghdad (Iraq). American
Journal of Educational Research, 2(8): 658-662.
7. Işın AKYAR, Simge CAN (2013). Rapid identification of Aeromonas species in stool samples with chromogenic media and matrixassisted laser desorption ionization–time of flight mass spectrometry: an institutional experience. Turkish Journal of Medical Sciences, 43: 388-
392.
8. Jimenez-Lopez C, Carpena M, Lourenço-Lopes C, Gallardo-Gomez M, Lorenzo J M, Barba F J, Simal-Gandara J (2020). Bioactive compounds
and quality of extra virgin olive oil. Foods, 9(8): 10-14.
9. Mishyna M, Mozgova Y, Kovalenko N, Zamaziy T (2018). Standard protocols to laboratory classes in special microbiology for the II and III year English media students of medical and dentistry faculties, (Part 3).
10. Munshi M, Arya P, Kumar P (2020). Physicochemical analysis and fatty acid profiling of fenugreek (Trigonella Foenum-Graecum) seed oil
using different solvents. Journal of Oleo Science, 69(11): 1349-1358.
11. Murthy A M, Sullivan M J, Nhu N T K, Lo A W, Phan M D, Peters K M, Sweet M J (2019). Variation in hemolysin A expression between
uropathogenic Escherichia coli isolates determines NLRP3‐dependent vs.‐independent macrophage cell death and host colonization. The
FASEB Journal, 33(6): 7437-7450.
12. Patel K K, Tripathi M, Pandey N, Agrawal A K, Gade S, Anjum M M, Singh S (2019). Alginate lyase immobilized chitosan nanoparticles of
ciprofloxacin for the improved antimicrobial activity against the biofilm associated mucoid P. aeruginosa infection in cystic
fibrosis. International journal of pharmaceutics, 563: 30-42.
13. Saranraj P V, Durga D (2017). Essential oils and its antimicrobial properties-A Review . Life Science Archives, 3(2): 994-1011
14. Surya K S, Basanta K D, Bibhuti B P (2014). Isolation, biochemical characterization, antibiotic susceptibility study of Aeromonas hydrophila
isolated from freshwater fish. Int. J. Curr.Microbiol. App. Sci., 3(12): 259-267.
15. Urmi U L, Nahar S, Rana M, Sultana F, Jahan N, Hossain B, Haque M (2020). Genotypic to Phenotypic Resistance Discrepancies Identified
Involving β-Lactamase Genes, bla KPC, bla IMP, bla NDM-1, and bla VIM in Uropathogenic Klebsiella pneumoniae. Infection and drug
resistance, 2020(13): 2863-2875.