QUALITY ASSESSMENT OF SPUTUM GRAM STAIN IN RELATION TO SPUTUM CULTURE FOR LOWER RESPIRATORY TRACT INFECTIONS IN A TERTIARY CARE CENTRE KANPUR
Main Article Content
Keywords
LRTI, Modified Bartlett’s Criteria, Klebsiella, Pseudomonas spp etc.
Abstract
Background: Lower respiratory tract infections (LRTIs) are among the most common infectious disease and responsible for the cause of morbidity and mortality worldwide. Microscopic examination of sputum is the most commonly followed method in the laboratory for diagnosing lower respiratory tract infections (LRTI).
Aim and Objectives:- Quality Assessment of Sputum Gram Stain in Relation to Sputum Culture for lower Respiratory Tract Infections in a Tertiary Care Centre Kanpur.
Material & Methods: - This study was a Cross- sectional study carried out at the Department of Microbiology, Rama Medical College Hospital & Research College Kanpur, and Uttar Pradesh, India from January 2021 to December 2021. All 150 sputum samples were collected from patients in our hospital during the study period and processed in the central laboratory. Repeated sputum samples from the same patient and samples received from pediatric age group were excluded from this study. Samples were evaluated by gross appearance and subjectively categorized into mucus (mucus strands present) and watery (saliva present).
Results: - In this study total 150 sputum samples, 105 (70%) samples were accepted according to modified Bartlett’s screening system and 45 (30%) samples were in the not acceptable category. Among acceptable category, 68(64.76%) samples were showed culture positivity. Among non-acceptable category, 11(24.44%) samples were showed culture positivity. The most common organism isolated was Klebsiella spp 29(42%), followed by Pseudomonas spp 23 (33.82%), Staphylococcus aureus 9(13.23%), Enterobacter spp 1(1.4%), Escherichia coli 1(1.4%), Citrobacter spp 1(1.4%) and Acinitobacter spp 1(1.4%). Streptococcus pyogenes 3(4.4%). Klebsiella spp. was the commonest isolated organism followed by Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes.
Conclusion: - Sputum quality assessment is a useful tool for recommended to receive good quality of sputum and do initial sputum screening for diagnosing clinically relevant lower respiratory tract infections.
References
2. Forbes BA, Sahm DF, Weissfeld AS. Bailey and Scott’s Diagnostic Microbiology; 12th edn. Missouri: Mosby; 2007.
3. Koneman EW, Allen SD, Janda WM, Schrekenberger PC. Color Atlas and Text book of Diagnostic Microbiology; 6th edn. Philadelphia: JB Lippencott; 2006.
4. Howard H Mizrachi and Paul N. Valenstein 1, 2. 1987 Randomized Trial Interpreting Sputum Quality in a Clinical Laboratory. J. Clin. Microbiol. Vol 25. No.12.
5. Joseph R Lentino and David A. Lucks. Non value of sputum culture in the management of lower respiratory tract infections. J. Clin. Microbiol. 1987; Vol 25. No 5.
6. Bindu Nair, Jenny Stapp, Lynn Stapp, Linda Bugni, Jill Van Dalfsen, and Jane L. Burns. Utility of Gram staining for evaluation of the quality of cystic fibrosis sputum samples. J. Clin. Microbiol. 2002; Vol 40. No 8.
7. Howard H Mizrachi and Paul N. Valenstein 1, 2. Randomized Trial Interpreting Sputum Quality in a Clinical Laboratory. J. Clin. Microbiol. 1987; Vol 25. No.12.
8. N Arora, MK Daga, R Mahajan, S Krishna Prakash and N Gupta. 2001. Microbiol Pattern of acute infective exacerbation of chronic obstructive airway disease in a hospital based study. Indian J chest dis allied sci 2001; 43: 157-62.
9. Mariraj j, et al Sputum Gram Stain Assessment in Relation to Sputum Culture for Respiratory Tract Infections in a Tertiary Care Hospital. Journal of Clinical and Diagnostic Research. 2011; Vol-5(8): 1699-1700.
10. Gorica Popova et al Sputum Quality Assessment Regarding Sputum Culture for Diagnosing Lower Respiratory Tract Infections in Children Open Access Macedonian Journal of Medical Sciences. 2019; 30: 7(12):1926-1930.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
