Purification and Characterization of Antifungal Protein from Bacteria
Main Article Content
Keywords
Fungi, Bacteria, Antifungal Protein, Purification, Characterization.
Abstract
Fungal infections have dramatically increased over the past few years, as evidenced by an increase in the number of deaths from fungal infections including AIDS, cancer, etc. New antifungal treatments must be developed to meet the global challenge of preventing fungal infection and mycotoxin contamination of food and feed. Due to their characteristics like diversity and function, antifungal spectrum, mechanism of action, high stability, and the availability of biotechnological production methods, antimicrobial peptides and
proteins (AMPs) with antifungal activity are attracting significant attention as natural antifungal agents. This study focused on the isolation of antifungal protein-producing microorganisms by using a crowded plate technique from the soil along with the purification of antifungal protein by methods like ammonium sulfate precipitation, solvent extraction, etc. along with this characterization of antimicrobial protein was carried out by polyacrylamide gel electrophoresis. Antifungal potential of purified protein was tested using
the agar well diffusion method against respective pathogenic fungi. Bacillus species having good antifungal activity have been isolated from the soil. Efficient extraction of antifungal compound produced by isolated Bacillus species has been done using ammonium sulfate precipitation as well as a solvent extraction method. The purified antifungal protein exhibits good antifungal activity against food-borne pathogens like Aspergillus niger and Candida albicance. Therefore, the isolated Bacillus strain and its potent antifungal
protein could be used in various food and biomedical applications.
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