Toxoplasmosis and Relation with Some Immunological Markers for Aborted Women in Thi-Qar Province
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Keywords
Toxoplasma gondii, Abortion, IFN-γ, CD4.
Abstract
Background: Toxoplasma gondii is a major cause of abortion in pregnant women. Toxoplasmosis infection in the mother during pregnancy is frequently associated with transplacental transmission of the parasite to the fetus. The purpose of this study was to determine the serum level concentrations of (CD4 and IFN-)in patients with toxoplasmosis and control groups, aims of this study determine the serum level concentration of (CD4, IFNγ) in patients with toxoplasmosis and control groups. and compare the different concentrations of (CD4, IFN) in acute, chronic, and control groups.
Materials and MethodsFrom September 2020 to May 2021, a case-control study was carried out in ThiQar province to estimate the role of Toxoplasmosis in the occurrence of abortion among pregnant women. The current study included 120 aborted women as well as 20 healthy women as controls (Non pregnant and have no clinical history of abortion). All of these cases involved only females of reproductive age (16-44 years). Toxo-IgM and Toxo-IgG antibodies were tested first with a latex agglutination test (LAT) to detect positive samples, followed by an Enzyme Linked Immunesorbent Assay (ELISA) to detect IgG and IgM antibodies in both groups. CUSABIO method for measuring human interferon (IFN-) and 2- human cluster of differentiation 4 (CD4) (USA).
Results: IFN-γ values of a patient with Toxo- IgG, and Toxo- IgM were (31.4&38.64) respectively which is significantly higher than the control value )28.80( CD4 value in a patient with IgM was (3.045) which is significantly higher than the control patient value (2.925). Also, CD4 value of patients with Toxo IgG (4.457) was higher than control with a significant value.
Conclusion: The concentration of IFN-Y was increase in acute infection and decrease in chronic of toxoplasmosis . The concentration of CD4 were increased with chronic infection compared with acute.
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