ANALYSIS OF WOUND AREA WITH TOPICAL THERAPY OF PROBIOTICS & LOW LEVEL LASER THERAPY IN RATS
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Abstract
Delayed wound healing is a major clinical concern. Low-level laser therapy (LLLT) at 660 nm and topical probiotics such as Streptococcus thermophilus have shown potential in accelerating tissue repair. However, comparative evidence between these modalities remains limited.
Objective: To evaluate and compare the effects of LLLT, Streptococcus thermophilus probiotic, and normal saline on wound healing progression over 21 days.
Methods: This experimental study included three groups receiving topical treatment: Group A (Control: normal saline), Group B (LLLT: 660 nm, 100 mW, 10 J/cm² for 30 s), and Group C (Probiotic: Streptococcus thermophilus). Wound samples were collected on days 3, 7, 14, and 21. Comparative analyses were performed between A & B, A & C, and B & C on selected days.
Results: Significant differences in wound healing were observed among all groups on days 3, 7, and 14. Both LLLT (Group B) and probiotic therapy (Group C) demonstrated faster wound contraction and improved tissue characteristics compared to the control. On day 21, no significant difference was found between Groups B and C, and both showed complete wound closure, whereas Group A exhibited delayed healing.
Conclusion: LLLT and Streptococcus thermophilus probiotic treatments significantly enhance wound healing compared to normal saline. By day 21, both modalities achieve comparable and complete wound closure, indicating their effectiveness as potential therapeutic options.
References
2. Kumar P, Das A, Banerjee P. Structural and functional aspects of the skin: a comprehensive review. Dermatol Clin Rev. 2022;8(2):112-126.
3. Lee M, Wong K. Advances in understanding the wound healing cascade. Int J Mol Med Sci. 2021;19(1):85-102.
4. Ahmed S, Malik R, Thompson G. The impact of diabetes on wound healing: mechanisms and clinical implications. Diabetes Wound Care J. 2023;14(2):312-329.
5. Sharma V, Patel N. Role of oxidative stress in delayed wound healing in diabetes. J Med Biochem Sci. 2022;10(4):221-238.
6. Goyal S, Khan H, Saxena R. Influence of reactive oxygen species and glycation in diabetic wound healing. Mol Biol Res. 2021;15(3):117-132.
7. Fernandez J, Ramos L, Costa D. Low-level laser therapy and its role in skin regeneration. Laser Ther J. 2023;18(1):45-62.
8. Mendes F, Oliveira P, Santos M. Effectiveness of photobiomodulation in chronic wounds: a systematic review. Photomed Laser Ther. 2022;17(3):98-112.
9. Rodriguez R, Kim B, Chen L. Low-level laser therapy in wound management: a meta-analysis. Clin Lasers Med Surg. 2023;25(2):73-91.
10. Park H, Choi Y, Lim J. The role of probiotics in wound healing: recent findings and future directions. J Microbiol Health Sci. 2023;29(1):156-172.
11. Jung S, Kang H, Lee E. Streptococcus thermophilus and its potential in skin barrier enhancement. J Cosmet Dermatol Sci. 2022;14(3):88-102.
12. Chen X, Lin M. Antimicrobial and skin-regenerating effects of probiotics in wound healing. Front Microbiol Med. 2021;20(4):215-231.
13. Takagi M, Nishimura Y, Fukuda T. Clinical effects of Streptococcus thermophilus in wound healing: a randomized controlled trial. J Clin Wound Care. 2023;11(2):125-140.
14. Özer H, İnci MA. Effect of low-level laser therapy in wound healing of primary molar teeth extraction. BMC Oral Health. 2024;24(1):348. doi:10.1186/s12903-024-05258-
15. Parmadiati AE, Putri YK, Zahra IA, Zulfa DR. Effectiveness of low-level laser therapy on oral wound healing: A scoping review. World J Adv Res Rev. 2024;24(2):2351–2361.
16. Xu Y, Lin Y, Gao S, Shen J. Study on mechanism of release oxygen by photo-excited hemoglobin in low-level laser therapy. Lasers in medical science. 2018;33(1):135-9.
17. Lombardi F, Augello FR, Artone S, et al. Efficacy of probiotic Streptococcus thermophilus in counteracting TGF-β1-induced fibrotic response in normal human dermal fibroblasts. J Inflamm (Lond). 2022;19:3. doi:10.1186/s12950-022-00294-2
18. Ebrahimi P, Hadilou M, Naserneysari F, et al. Effect of photobiomodulation in secondary intention gingival wound healing—a systematic review and meta-analysis. BMC Oral Health. 2021;21(1):258. doi:10.1186/s12903-021-01611-2
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Dr Devi Kumari
Assistant Professor, Department of Anatomy, Al Tibri Medical College Isra University Karachi, Pakistan
Dr Muhammad Asif
Professor, PT Chairman, Department of Rehabilitation and Health Sciences, Nazeer Hussain University Karachi, Pakistan.
Dr Asad Raza Jiskani
Professor of community Medicine, Isra University Karachi, Pakistan
Dr Jaswanti Kumari
Department of Gynae and Obstetric, FCPS Part 2 Training, DUHS Ojha Campus, Karachi, Pakistan
Dr Nadeem ul Haque
Professor, Department of Biochemistry, Al Tibri Medical College Karachi, Pakistan
Dr Aziza Khanam
Professor of Biochemistry, Additional Director Postgraduate Affairs, Isra University Karachi, Pakistan
Dr.Hina Khan
Professor, Deputy Director Research and Postgraduate Affairs, Deputy Director Research and Postgraduate Labs, Pakistan
