ASSESSMENT OF OXIDATIVE STRESS IN PATIENTS WITH TYPE 2 DIABETES MELLITUS IN CORRELATION TO HBA1C
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Keywords
TAC (total antioxidant capacity), MDA (malondialdehyde), HbA1c (glycosylated hemoglobin), Oxidative Stress, Diabetes Mellitus.
Abstract
Introduction: Diabetes mellitus is a complex disorder characterized by either insufficient insulin production or ineffective insulin action. Chronic hyperglycemia can lead to various diabetic complications, including neuropathy, nephropathy, and retinopathy. Oxidative stress is a primary factor contributing to these complications.
Aim: This study aims to assess the oxidative stress levels in patients with type 2 diabetes mellitus (type 2 DM) in comparison to healthy individuals.
Materials & Methods: The research involved 140 participants, comprising 70 individuals diagnosed with type 2 DM (Diabetic group) and 70 healthy controls (Control group). Following a thorough medical history assessment, 5 ml of venous blood was drawn from each participant in both groups and sent to a central laboratory for the evaluation of blood glucose parameters (HbA1c%, fasting blood sugar mg/dl, and postprandial blood sugar mg/dl) as well as oxidative stress markers (total antioxidant capacity in mmol/dl and malondialdehyde in mmol/dl). The collected data were analyzed using SPSS software version 23.0.
Results: The diabetic group exhibited a significant increase in HbA1c, fasting blood sugar, and postprandial blood sugar levels when compared to the healthy individuals. Furthermore, oxidative stress levels were found to be elevated in diabetic patients, indicated by an increase in mean malondialdehyde and a decrease in total antioxidant capacity (P<0.05). A strong positive correlation was observed between HbA1c and malondialdehyde.
Conclusion: The diabetic group demonstrated elevated blood glucose levels alongside increased oxidative stress. The study also established a significant correlation between glycemic levels and oxidative stress, as evidenced by the rise in malondialdehyde levels with increasing blood glucose levels.
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Dr. Supriya
Professor, Department of Biochemistry,PES University Institute of Medical Sciences and Research, Bangalore, Karnataka,India.
Dr.Raksha Hebbar K
Associate Professor, Department of Physiology, PES University Institute of Medical Sciences and Research,Bangalore, Karnataka, India.
Dr M Anuswaru
Assistant Professor, Department of Biochemistry, PES University Institute of Medical Sciences and Research, Bangalore, Karnataka, India.
Rishi KP
Student of MBBS, PES University Institute of Medical Sciences and Research, Bangalore, Karnataka, India.