Design of Methodologies for the Determination of Selenium and Mercury in Samples of Environmental Interest by Solid Phase Spectrophotometry

Main Article Content

Muhammed Abdel Hasan Shallal

Keywords

Selenium and Mercury, Quantitative analysis, colorimetry methods, method development, Solid Phase Spectrophotometry

Abstract

Selenium is a micronutrient with biological, antioxidant, and catalytic properties. Mercury's potential for volatilization and methylation makes its environmental behaviour both interesting and concerning.Because organic compounds can bioaccumulate in the food chain, they are the most toxic. These analytes are extremely rare in nature. It is critical to have an analytical method that combines a speciespreserving sample treatment with a system that allows for detection at low concentration levels. There are effective methods and techniques for detecting these species, but they require complex instrumentation and installations, making them impractical for use outside of the lab. Solid phase spectrophotometry is up-front and sensitive method for determining these elements. Overall, the goal of this research was to develop an analytical technique, such as solid phase spectrophotometry, that can measure selenium and mercury in environmental samples in a reliable and safe manner. The technique is to fix a coloured analyte-containing species to a solid support with specific properties. After the coloured complex has been fixed, spectral measurements are taken to quantify the analyte.Organic and inorganic analytes have been measured due to the versatility of these methods. The parameters for determining selenium in foliar samples and mercury in water samples are proposed and standardised using solid phase spectrophotometry. The results of this method are validated to ensure accuracy. The precision, linear range, detection limit, and quantification limit all demonstrate dependability. 1-100 mg/L linear range, the detection limit was 1.92 mg/L, while the quantification limit was 6.41 mg/L. The method's repeatability and reproducibility tests revealed a low degree of dispersion.

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References

1. Amin, Alaa. (2014). Speciation of Selenium in Environmental Samples by Solid-Phase Spectrophotometry Using 2,3-Dichloro-6-(2,7-
dihydroxy-naphthylazo)quinoxaline. Journal of AOAC International. 97. 586-92.10.5740/jaoacint.11-002.
2. Clarkson, Thomas & Magos, Laszlo & Myers,Gary. (2003). The Toxicology of Mercury —Current Exposures and Clinical Manifestations.
The New England journal of medicine. 349. 1731-7. 10.1056/NEJMra022471.
3. El-Ramady, Hassan & domokos-szabolcsy, Eva & Shalaby, Tarek & Joe, Prokisch & Fári, Miklós. (2015). Selenium in Agriculture: Water, Air, Soil, Plants, Food, Animals and Nanoselenium. 10.1007/978-3-319-11906-9_5.
4. Gavrilenko, Nataliya & Saranchina, Nadezhda & Fedan, D. & Gavrilenko, Mikhail. (2017). Solidphase spectrophotometric iodometric
determination of nitrite and selenium(IV) using a polymethacrylate matrix. Journal of Analytical Chemistry. 72. 546-550.
10.1134/S1061934817030054.
5. Jenkins DJA, Kitts D, Giovannucci EL, SahyePudaruth S, Paquette M, Blanco Mejia S, Patel D, Kavanagh M, Tsirakis T, Kendall CWC, Pichika SC, Sievenpiper JL. Selenium, antioxidants, cardiovascular disease, and all-cause mortality: a systematic review and meta-analysis of randomized controlled trials. Am J Clin Nutr.2020 Dec 10;112(6):1642-1652. doi:10.1093/ajcn/nqaa245.
6. Kadis, Rouvim. (2011). Measurement uncertainty and chemical analysis. Journal of Analytical Chemistry. 63. 95-100.
10.1134/S1061934808010188.
7. Kompany-Zareh, Mohsen & Mansourian, Mahboubeh & Ravaee, Fariba. (2002). Simple method for colorimetric spot-test quantitative
analysis of Fe(III) using a computer controlled hand-scanner. Analytica Chimica Acta. 471. 97-104. 10.1016/S0003-2670(02)00871-1.
8. Krystyna Pyrzyńska (2002). Determination of Selenium Species in Environmental Samples.Microchimica Acta. 140. 55-62. 10.1007/s00604-001-0899-8.
9. Pellerano, R.G.; Romero, C.H.; Acevedo, A.H.; Vazquez, F.A. (2007); A low-cost method for the determination of trace-level copper in matrices of environmental interest by solid-phase spectrophotometry (EFS). Chemistry. Nova: 30(8), 2020-2024.
10. Saffaj, Taoufiq & IHSSANE, Bouchaib & Jhilal, F. & Bouchafra, Houda & Laslami, S. & Sosse, Saad. (2013). An overall uncertainty approach for the validation of analytical separation methods.The Analyst. 138. 10.1039/c3an00519d.
11. Serra, A.M. & Estela, Jose & Coulomb, Bruno & Boudenne, Jean-Luc & Cerdà, Víctor. (2010). Solid phase extraction - Multisyringe flow
injection system for the spectrophotometric determination of selenium with 2,3- diaminonaphthalene. Talanta. 81. 572-7.
10.1016/j.talanta.2009.12.045.
12. Soruraddin, Mohammad & Heydari, Rouhollah & Puladvand, Morteza & Zahedi, Mir. (2011). A New Spectrophotometric Method for
Determination of Selenium in Cosmetic and Pharmaceutical Preparations after Preconcentration with Cloud Point Extraction. International journal of analytical chemistry. 2011. 729651. 10.1155/2011/729651.
13. Tinggi U. Selenium: its role as antioxidant in human health. Environ Health Prev Med. 2008 Mar;13(2):102-8. doi: 10.1007/s12199-007-
0019-4. Epub 2008 Feb 28.
14. Wu, D.; Huang, W.; Lin, Z.; Duan, C.; He, C.; Wu, S.; Wang, D. (2008); Highly sensitive multiresponsive chemosensor for selective detection of Hg2+ in natural water and different monitoring environments. Inorganic Chemistry:47 (16), 7190-7201.
15. Mahdi, E. M., & Mustafa, M. A. (2022). Effect of different concentrations of extract of Urtica dioica and Cladosporium cladosporiodes on Tribolium castaneum or: Coleoptera after 24-48 hours of exposure in Samarra City/Iraq. HIV Nursing, 22(2), 3207-3210.
16. Mustafa, M. A., Kadham, S. M., Abbass, N. K., Karupusamy, S., Jasim, H. Y., Alreda, B. A., ... & Ahmed, M. T. (2023). A novel fuzzy M-transform technique for sustainable ground water level prediction. Applied Geomatics, 1-7.
17. Kadham, S. M., Mustafa, M. A., Abbass, N. K., & Karupusamy, S. (2022). IoT and artificial intelligence–based fuzzy-integral N-transform for sustainable groundwater management. Applied Geomatics, 1-8.