A COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFECTS OF HERBAL ROOT IRRIGANT CARVACROL VERSUS SODIUM HYPOCHLORITE USING PASSIVE AND ULTRASONIC IRRIGATION TECHNIQUE – AN IN VITRO STUDY

Main Article Content

Dr. Kanika Jain
Dr.Aakriti
Dr. Anukriti Pathak

Keywords

Enterococcus faecalis, Biomechanical Preparation, Antimicrobial Efficacy, Azadirachta indica, Sodium hypochlorite.

Abstract

AIM & OBJECTIVE- Microorganisms are the actual cause of all the pathologies of the pulpal and periapical tissues. Enterococcus faecalis is the most commonly found bacteria in failed root canal treatments. Chemical irrigation of canals along with biomechanical preparation helps in the elimination of microorganisms. The present study was aimed to evaluate the antimicrobial effect of herbal root canal irrigant Azadirachta indica with sodium hypochlorite (NaOCl) using passive and ultrasonic irrigation technique.


MATERIAL & METHODS - The antimicrobial efficacy of irrigant was assessed by counting the colony forming units of E. faecalis which was taken from root surface by the sterile paper point and inoculated on blood agar and incubated for 24 hours at 370C. It was done by incubating irrigated teeth samples with paper point for 30 minutes. Then, those paper points were used for inoculating the E. faecalis colonies on the blood agar. Blood agar plates were incubated for 24 hour at 370C. Then, Colony forming unit and percent reduction can be calculated.


RESULTS – Azadirachta indica has antimicrobial effect against E faecalis but it can not be ultrasonically activated.


CONCLUSION – Due to various toxic effects of sodium hypochlorite, Azadirachta indica can be used as an intracanal irrigant as it has antimicrobial effects against E faecalis.

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