IN-VITRO ANTIOXIDANT AND NEUROPROTECTIVE POTENTIAL OF MUSA PARADISIACA FRUIT PEEL EXTRACT IN SH-SY5Y HUMAN NEUROBLASTOMA CELLS
Main Article Content
Keywords
Musa paradisiaca, Anti-oxidant, SH-SY5Y cells, Neuroprotection, Parkinson’s disease.
Abstract
Parkinson’s disease (PD) is the second most prevalent neurodegenerative disorder after Alzheimer’s marked by dopaminergic neuronal loss linked to oxidative stress. The aim of present study was to investigate the in-vitro antioxidant and neuroprotective potential of the ethanolic extract of Musa paradisiaca fruit peel (EEMP) and its cytoprotective efficacy in SH-SY5Y human neuroblastoma cells.
Methods and Materials: Fruit peels of Musa paradisiaca were shade-dried, extracted with ethanol, and analyzed for physicochemical and phytochemical properties. Total phenolics, flavonoids, vitamin C, and vitamin E were quantified. Anti-oxidant activity was determined by the DPPH assay, and neuroprotective effects were assessed by the MTT assay on SH-SY5Y cells.
Results & Discussion: Quantitative analysis of EEMP revealed the presence of a significant amount of phenols, flavonoids, vitamin C and E. EEMP showed moderate antioxidant activity with IC₅₀ of 80.65 µg/mL and concentration-dependent cytotoxicity (IC₅₀ = 70.13 µg/mL) against SH-SY5Y cells, with morphological evidence of apoptosis at higher concentrations. The potent antioxidant and neuroprotective effects of EEMP may be attributed to its rich phenolic and vitamin content, which effectively counteracts oxidative stress and helps maintain neuronal integrity. Musa paradisiaca fruit peel may serve as a promising natural therapeutic candidate for the prevention and management of oxidative stress–related neurodegenerative disorders, such as Parkinson’s disease.
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Sumithira George
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112,
Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Abitha Sri Ramamurthy
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112, Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Ragul Ravi
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112, Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Abirami Gopi
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112, Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Abarna Shanmugam
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112, Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Chandru Elumalai
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112, Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Raguraman ponraj
Department of Pharmacology, The Erode College of Pharmacy and Research Institute, Erode – 638112, Tamil Nadu, India. (Affiliated to The Tamil Nadu Dr MGR Medical University Chennai).
Krishnamoorthy Balakrishnan
Department of pharmaceutics, Sanjivani College of pharmaceutical Sciences, Khetri-333503, Rajasthan. India (Affiliated to Rajasthan university of Health Sciences, Jaipur)
Suresh Ratinasamy
Department of pharmacology, Greensmed Labs, Chennai– 600097, Tamil Nadu, India.