CIMTris METHOD FOR DETECTING CARBAPENEMASE IN CLINICALLY ISOLATED NLFs AMIDST RISING DRUG RESISTANCE
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Keywords
Carbapenemase producing organism, CIMTris Method, Non-lactose fermenting Gram-negative bacilli, Multidrug-resistance, Carbapenemase, Phenotypic detection.
Abstract
Background: Carbapenemase-producing non-lactose fermenting gram-negative bacilli (NLFGNB), especially Acinetobacter baumannii and Pseudomonas aeruginosa, pose a major threat in healthcare settings due to rising antimicrobial resistance and limited treatment options.
Objectives: This study aimed to investigate the prevalence of carbapenemase production in NLF bacteria isolated from clinical specimens.
Methods: A total of 207 clinical NLFGNB isolates were tested using the CIMTris method, which involves incubating isolates in Tris-HCl buffer with meropenem discs, followed by zone-of-inhibition analysis on E. coli-inoculated agar plates.
Results: Of 207 isolates, 28.0% were carbapenemase-positive, 10.6% indeterminate, and 60.9% negative. Highest positivity was seen in A. baumannii (48.1%), followed by P. aeruginosa (15.7%) and A. lwoffii (16%). The CIMTris method showed 97.6% sensitivity and 92.6% specificity.
Conclusion: CIMTris is a rapid, cost-effective, and sensitive phenotypic method for detecting carbapenemase in NLFGNBs, especially effective for A. baumannii. However, molecular testing is recommended for indeterminate results to ensure early detection and containment of emerging resistance in high-risk hospital settings.
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Pinaki Chattopadhyay
Associate Professor, Department of Microbiology, Midnapore Medical College & Hospital, Vidyasagar Road, Paschim Medinipur, Pin-721101, West Bengal, India.
Avishek Roy
Molecular Biologist, VRDL Laboratory, Department of Microbiology, Midnapore Medical College & Hospital, Vidyasagar Road, Paschim Medinipur, Pin-721101, West Bengal, India.
Sabyasachi Saha
Microbiologist, C&DST Laboratory (under N.T.E.P), Department of Microbiology, Midnapore Medical College & Hospital, Vidyasagar Road, Paschim Medinipur, Pin-721101, West Bengal, India.
Sayantanu Jana
Molecular Biologist, VRDL Laboratory, Department of Microbiology, Midnapore Medical College & Hospital, Vidyasagar Road, Paschim Medinipur, Pin-721101, West Bengal, India.
Ranadeep Ghosh
Professor, Department of Microbiology, Midnapore Medical College & Hospital, Vidyasagar Road, Paschim Medinipur, Pin-721101, West Bengal, India.
Kheya Mukherjee
Professor & Head, Department of Microbiology, Midnapore Medical College & Hospital, Vidyasagar Road, Paschim Medinipur, Pin-721101, West Bengal, India.