A Comparative Study On Different Methods For MRSA, Bacteraemia, Staphylococcus aureus, mecA Detection In Staphylococcus Aureus Isolates From Bacteraemia Patients.
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Keywords
MRSA, Bacteraemia, Staphylococcus aureus, mecA
Abstract
Introduction: Methicillin resistant Staphylococcus aureus bacteraemia cases are distributed throughout the globe. There is increased mortality and morbidity in cases of MRSA bacteraemia. MRSA strains are resistant to all antibiotics related to penicillin and beta lactam groups. Detection of MRSA is challenge as there are multiple way with different sensitivity and specificity. Therefore, this study is planned to compare different ways for MRSA detection.
Material & Methods: For a period of two years June 2017 to May 2019, 162 Staphylococcus aureus isolates from blood cultures received from different critical care units were studied. MRSA was phenotypically detected by Cefoxitin Disc Diffusion, oxacillin broth microdilution, oxacillin agar diffusion methods. Genotypic detection was done by mec A, mec C PCR.
Results: Out of 162 Staphylococcus aureus isolates screened, methicillin resistance was detected in 93 (57.4%) isolates by cefoxitin disk diffusion method, 84 (51.8%) isolates by the oxacillin broth microdilution 82(50.61%) isolates by Oxacillin Agar Diffusion method. mec A was detected in 94 strains. mec C was not detected.
Conclusion: MRSA detection by Cefoxitin Disk diffusion testing is reliable as genotyping detection is difficult to be done in the resource constraint setting.
References
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Dr Aditi Garg
Assistant Professor, Microbiology, ASMC Hardoi 0003-0315-3195, Uttar Pradesh
Dr Rani Kumari Jaiswal
Assistant Professor, Microbiology, Naraina Institute of Medical Sciences, Kanpur, Uttar Pradesh
Dr. Dhruv Agarwal
Associate Professor, Community Medicine, ASMC Hardoi, Uttar Pradesh
Dr. Anuragini Verma
Assistant Professor, RMLIMS, Lucknow 0001-9874-0565, Uttar Pradesh
Dr. Prashant Gupta
Professor, Microbiology, KGMU Lucknow 0002-6925-3546, Uttar Pradesh
Dr. Vimala Venkatesh
Professor, Microbiology, KGMU Lucknow 0002-6925-3546, Uttar Pradesh
