IMMEDIATE EFFECTS OF STRUCTURED DEEP BREATHING ON CARDIOVASCULAR FUNCTION, AUTONOMIC REGULATION, AND PERCEIVED STRESS IN HEALTHY ADULTS

Main Article Content

Mallipeddi Kalyani
Jyothi Ashok Kumar
Praveen Kumar Panuganti

Keywords

Deep breathing, Heart rate variability, Blood pressure, Respiratory rate, Oxygen saturation, Perceived stress, Autonomic balance, Healthy adults

Abstract

Background: Deep breathing exercises are a simple, non-pharmacological technique shown to enhance autonomic balance, improve cardiovascular function, and reduce psychological stress. However, evidence on the acute effects of short-term, structured deep breathing in healthy adults remains limited. This study aimed to evaluate the impact of short-term deep breathing exercises on heart rate variability, blood pressure, respiratory function, oxygen saturation, and perceived stress.


Methods: A total of 100 healthy adults were randomly assigned to two groups: a Control group (n = 50; no specific intervention) and an Experimental group (n = 50; performed a standardized deep breathing protocol). Physiological parameters including systolic and diastolic blood pressure, heart rate, heart rate variability (RMSSD), respiratory rate, and oxygen saturation were measured at baseline and post-intervention. Psychological stress was assessed using the Perceived Stress Scale (PSS). Independent and paired t-tests were used for between- and within-group comparisons, respectively, with significance set at p < 0.05.


Results: At baseline, both groups were comparable across all parameters. Following the intervention, the experimental group demonstrated significant reductions in systolic blood pressure (–5.28 mmHg, p = 0.0045), diastolic blood pressure (–4.06 mmHg, p = 0.0025), heart rate (–4.28 bpm, p = 0.023), and respiratory rate (–2.03 breaths/min, p < 0.0001). Heart rate variability improved markedly (RMSSD: +8.39 ms, p = 0.0001), and oxygen saturation increased (+0.93%, p < 0.0001). A significant reduction in perceived stress was also observed (–4.79 points, p < 0.0001). No significant changes were found in the control group.


Conclusion: Short-term deep breathing exercises produced measurable improvements in cardiovascular regulation, autonomic balance, respiratory efficiency, and psychological well-being among healthy adults. These findings highlight the potential of brief, structured deep breathing as an effective, low-cost, and accessible strategy for promoting holistic health in everyday life.

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References

[1] Shaffer F, Ginsberg JP. An overview of heart rate variability metrics and norms. Front Public Heal 2017;5:258.
[2] McEwen BS. Physiology and Neurobiology of Stress and Adaptation: Central Role of the Brain. Physiol Rev 2007;87:873–904. https://doi.org/10.1152/physrev.00041.2006.
[3] Lehrer PM, Gevirtz R. Heart rate variability biofeedback: how and why does it work? Front Psychol 2014;5. https://doi.org/10.3389/fpsyg.2014.00756.
[4] Garg P, Mendiratta A, Banga A, Bucharles A, Piccoli MVF, Kamaraj B, et al. Effect of breathing exercises on blood pressure and heart rate: A systematic review and meta-analysis. Int J Cardiol Cardiovasc Risk Prev 2024;20:200232. https://doi.org/10.1016/j.ijcrp.2023.200232.
[5] Yeh T-T, Ho Y-C. Immediate effects of structured and natural deep breathing on heart rate variability and blood pressure in community-dwelling older adults. Exp Gerontol 2024;198:112644. https://doi.org/10.1016/j.exger.2024.112644.
[6] Catela D, Santos J, Oliveira J, Franco S, Mercê C. Heart Rate Variability, Blood Pressure and Peripheral Oxygen Saturation during Yoga Adham and Mahat Breathing Techniques without Retention in Adult Practitioners. J Funct Morphol Kinesiol 2024;9:184. https://doi.org/10.3390/jfmk9040184.
[7] Fincham GW, Strauss C, Montero-Marin J, Cavanagh K. Effect of breathwork on stress and mental health: A meta-analysis of randomised-controlled trials. Sci Rep 2023;13:432. https://doi.org/10.1038/s41598-022-27247-y.
[8] Shao R, Man ISC, Lee TMC. The Effect of Slow-Paced Breathing on Cardiovascular and Emotion Functions: A Meta-Analysis and Systematic Review. Mindfulness (N Y) 2024;15:1–18. https://doi.org/10.1007/s12671-023-02294-2.
[9] Zou Y, Zhao X, Hou Y-Y, Liu T, Wu Q, Huang Y-H, et al. Meta-Analysis of Effects of Voluntary Slow Breathing Exercises for Control of Heart Rate and Blood Pressure in Patients With Cardiovascular Diseases. Am J Cardiol 2017;120:148–53. https://doi.org/10.1016/j.amjcard.2017.03.247.
[10] Yau KK-Y, Loke AY. Effects of diaphragmatic deep breathing exercises on prehypertensive or hypertensive adults: A literature review. Complement Ther Clin Pract 2021;43:101315. https://doi.org/10.1016/j.ctcp.2021.101315.
[11] Laborde S, Allen MS, Borges U, Dosseville F, Hosang TJ, Iskra M, et al. Effects of voluntary slow breathing on heart rate and heart rate variability: A systematic review and a meta-analysis. Neurosci Biobehav Rev 2022;138:104711. https://doi.org/10.1016/j.neubiorev.2022.104711.
[12] Jensen MK, Andersen SS, Andersen SS, Liboriussen CH, Kristensen S, Jochumsen M. Modulating Heart Rate Variability through Deep Breathing Exercises and Transcutaneous Auricular Vagus Nerve Stimulation: A Study in Healthy Participants and in Patients with Rheumatoid Arthritis or Systemic Lupus Erythematosus. Sensors 2022;22:7884. https://doi.org/10.3390/s22207884.
[13] Malhotra V, Bharshankar R, Ravi N, Bhagat OL. Acute Effects on Heart Rate Variability during Slow Deep Breathing. Mymensingh Med J MMJ 2021;30:208–13.
[14] Ali MK, Liu L, Hussain A, Zheng D, Alam M, Chen J-H, et al. Root mean square of successive differences is not a valid measure of parasympathetic reactivity during slow deep breathing. Am J Physiol Integr Comp Physiol 2023;324:R446–56. https://doi.org/10.1152/ajpregu.00272.2022.
[15] Indra Frana Jaya KK. Effect of Diaphragmatic Breathing Exercise on Respiratory Rate and Oxygen Saturation in patients with Bronchial Asthma. Indones J Heal Serv 2024;1:18–24. https://doi.org/10.63202/ijhs.v1i1.5.
[16] Mazur MD, Hamner JW, Anand AN, Taylor JA. Cardiorespiratory Effects of Yogic Versus Slow Breathing in Individuals with a Spinal Cord Injury: An Exploratory Cohort Study. J Integr Complement Med 2024;30:878–85. https://doi.org/10.1089/jicm.2023.0641.
[17] Burge AT, Gadowski AM, Jones A, Romero L, Smallwood NE, Ekström M, et al. Breathing techniques to reduce symptoms in people with serious respiratory illness: a systematic review. Eur Respir Rev 2024;33:240012. https://doi.org/10.1183/16000617.0012-2024.
[18] Hopper SI, Murray SL, Ferrara LR, Singleton JK. Effectiveness of diaphragmatic breathing for reducing physiological and psychological stress in adults: a quantitative systematic review. JBI Database Syst Rev Implement Reports 2019;17:1855–76. https://doi.org/10.11124/JBISRIR-2017-003848.
[19] Ma X, Yue Z-Q, Gong Z-Q, Zhang H, Duan N-Y, Shi Y-T, et al. The Effect of Diaphragmatic Breathing on Attention, Negative Affect and Stress in Healthy Adults. Front Psychol 2017;8. https://doi.org/10.3389/fpsyg.2017.00874.
[20] Tharion E, Samuel P, Rajalakshmi R, Gnanasenthil G, Subramanian RK. Influence of deep breathing exercise on spontaneous respiratory rate and heart rate variability: a randomised controlled trial in healthy subjects. Indian J Physiol Pharmacol 2012;56:80–7.