IDENTIFICATION OF UNCHARACTERIZED WUCHERARIA BANCROFTI PROTEINS USING PHYRE 2.0 AND INTERPRO SOFTWARE
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Abstract
METHOD: Globally, filariasis is one of the most common diseases effected by wucheraria bancrofti parasite. The two uncharacterized wucheraria bancrofti were inserted into phyre 2 for knowing protein homology and 3D structure of proteins and interpro used for understanding domain annotation, visualisation and family of protein. RESULTS: The 1st protein showed the highest confidence and coverage level of 100% followed by second protein, first protein is having 11% disorderness and majority of beta-helix and alpha-helix and 23% and 23% and 21% respectively. Whereas second protein has no disorderness with 26% alpha-helix. The two proteins visualization exhibited secondary structure and more than 50 amino acid residues for each protein. CONCLUSION: The second protein would be (o44786 ID) best target lead for wcheraria bancrofti. This research aims to understand the structure and function of two uncharacterized wcheraria bancrofti proteins (P35666 ID, o44786 ID) using bioinformatic methods in an aim to learn more about the parasite methods. The two uncharacterized proteins were given input into phyre 2 for determining the active site, domains 3D structures and sequence function. Interpro is also used to determine the SUPER FAMILY of given proteins.
References
2.World Health Organization. Lymphatic filariasis fact sheet. Geneva: WHO; [cited 2025 Sep 19]. Available from: https://www.who.int/news-room/fact-sheets/detail/lymphatic-filariasis
3.History, Epidemiology and Control of Filariasis. PLoS Neglected Tropical Diseases. 2011;5(4):e1185.
4.Canada.ca. Infectious Substances – Wuchereria bancrofti: Pathogen Safety Data Sheet. Ottawa: Government of Canada; [cited 2025 Sep 19]. Available from: https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/wuchereria-bancrofti.html
5.World Health Organization. Lymphatic filariasis. Geneva: WHO; 2023 [cited 2025 Sep 19]. Available from: https://www.who.int/news-room/fact-sheets/detail/lymphatic-filariasi.s
6.Taylor MJ, Hoerauf A, Bockarie M. Lymphatic filariasis and onchocerciasis. Lancet. 2010;376(9747):1175-85. doi:10.1016/S0140-6736(10)60586-7.
7.Chandy C, Thanikachalam S, Ramalingam S. Basic science of lymphatic filariasis. Indian J Urol. 2005;21(1):10-16. doi:10.4103/0970-1591.19552.
8.Nutman TB. Insights into the pathogenesis of disease in human lymphatic filariasis. Lymphat Res Biol. 2013;11(3):144-8. doi:10.1089/lrb.2013.0015.
9.Simonsen PE, Mwakitalu ME. Urban lymphatic filariasis. Parasitol Res. 2013;112(1):35-44. doi:10.1007/s00436-012-3226-x.
10.Michael E, Bundy DA, Grenfell BT. Re-assessing the global prevalence and distribution of lymphatic filariasis. Parasitology. 1996;112(4):409-28. doi:10.1017/S0031182000066646.
11Centers for Disease Control and Prevention. Parasites – Lymphatic Filariasis. Atlanta: CDC; 2022 [cited 2025 Sep 19]. Available from: https://www.cdc.gov/parasites/lymphaticfilariasis.
12. Chauhan N, Sharma R, Hoti SL. Identification and biochemical characterization of macrophage migration inhibitory factor-2 (MIF-2) homologue of human lymphatic filarial parasite, Wuchereria bancrofti. Acta Tropica. 2015;142:71-78. doi:10.1016/j.actatropica.2014.10.009
13.Sharma R, Hoti SL, Meena RL, Vasuki V, Sankari T, Kaliraj P. Molecular and functional characterization of macrophage migration inhibitory factor (MIF) homolog of human from lymphatic filarial parasite Wuchereria bancrofti. Parasitology Research. 2012;111(5):2035-2047. doi:10.1007/s00436-012-3051-2.
14. Non-mammalian MIF superfamily review. Immunology & Cell Biology. 2017;95(5):372-381. doi:10.1111/imcb.12003 (or similar – see “The non-mammalian MIF superfamily” PMC article).
15.Singh I, Kanichery A, Kotimoole CN, Modi PK, Prasad TSK, Hoti SL. Unpacking immune modulation as a site of therapeutics innovation for nematode parasite Wuchereria bancrofti: a temporal quantitative phosphoproteomics profiling of macrophage migration inhibitory factor 2. OMICS. 2024;28(3):125-137. doi:10.1089/omi.2024.0002.
16.Sharma R, Hoti SL, Meena RL, Vasuki V, Sankari T, Kaliraj P. Molecular and functional characterization of macrophage migration inhibitory factor (MIF) homolog of human from lymphatic filarial parasite Wuchereria bancrofti. Parasitology Research. 2012;111(5):2035-2047. doi:10.1007/s00436-012-3051-2.
17.Nematode Orthologs of Macrophage Migration Inhibitory Factor (MIF) Homologs. Pathogens. 2022;11(2):258. doi:10.3390/pathogens11020258.
18.Sureshan M, Prabhu D, Saraboji K, et al. Structure-based designing of anti-filarial molecules targeting the key antioxidant enzyme glutathione peroxidase (GPx) of Wuchereria bancrofti: an integrated in silico and in vitro approach. Computers in Biology and Medicine. 2025 Sep;195:110669. doi:10.1016/j.compbiomed.2025.110669.
19.Filarial selenium glutathione peroxidase: a probable immunodiagnostic marker for lymphatic filariasis. [Conference/Abstract or Journal]. [year]; [volume(issue)]:[page numbers]. (Exact details may need verifying).
20. Powell HR, Islam SA, David A, Sternberg MJE, et al. Phyre2.2: A community resource for template-based protein structure prediction. Journal of Molecular Biology. 2025;437(15):168960. doi:10.1016/j.jmb.2025.168960.
21. Cho Y, Jones BF, Vermeire JJ, Leng L, DiFedele L, Harrison LM, et al. Structural and functional characterisation of a secreted hookworm macrophage migration inhibitory factor (MIF) that interacts with the human MIF receptor CD74. J Biol Chem. 2007;282(34):23447-56.
22. Pastrana DV, Raghavan N, Fitzgerald P, Eisinger SW, Metz C, Bucala R, et al. Filarial nematode parasites secrete a homologue of the human cytokine macrophage migration inhibitory factor. Infect Immun. 1998;66(12):5955-63.
23. Swagata Ghosh, Nona Jiang, Laura Farr, Renay Ngobeni, Shannon Moonah. Parasite-Produced MIF Cytokine: Role in Immune Evasion, Invasion and Pathogenesis. Front Immunol. 2019;10:1995.56
24. Muthusamy S, Prabhu D, Saraboji K, et al. Structure-based designing of anti-filarial molecules targeting the antioxidant enzyme glutathione peroxidase of Wuchereria bancrofti: an integrated in silico and in vitro approach. Comput Biol Med. 2025;195:110669.
25. Veerapathran A, Dakshinamoorthy G, Gnanasekar M, Reddy MVR, Kalyanasundaram R. Evaluation of Wuchereria bancrofti glutathione S-transferase as a vaccine candidate for lymphatic filariasis. PLoS Negl Trop Dis. 2009;3(6):e457.
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Swathi varri
Assistant Professor, Department of Pharmaceutics, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh- India.
Dr. D Narendra
Principal & Professor, Department of Pharmaceutical analysis, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh – India.
Navya
Student, Department of Pharmacy, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh – India.
Sowjanya
Student, Department of Pharmacy, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh – India.
Rupa Sri
Student, Department of Pharmacy, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh – India.
Deepika
Student, Department of Pharmacy, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh – India
Johaparvaz
Student, Department of Pharmacy, VJ’s college of Pharmacy, Rajahmundry, Andhra Pradesh – India