AN APPROACH FOR AGE ESTIMATION WITH INCREMENTAL LINES FROM ROOT CEMENTUM OF HUMAN TEETH WITH LIGHT MICROSCOPE IN NORTH INDIAN POPULATION
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Keywords
Forensic odontology, Cementum, Age estimation, Incremental lines, Root cementum, Light microscopy, Ground tooth section
Abstract
Forensic age estimation plays one of the essential roles in the unknown human remains identification. Like in mass disasters and criminal investigations cases. Out of various odontological methods, analysis of incremental lines in root cementum has emerged as a non-destructive and reliable approach. Cementum, a mineralized avascular tissue covering the tooth root, continuously deposits throughout life in the form of alternating light and dark concentric bands, known as incremental lines or cemental annulations. This study explores the use of light microscopy to analyze these incremental lines for age estimation in the North Indian population. Ground sections of extracted teeth were prepared and examined under a light microscope to count the cemental annulations. The estimated age was then calculated by adding the eruption age of the respective tooth to the number of observed cemental lines. This method showed promising correlation between chronological and estimated age, supporting its validity and applicability in forensic contexts. The study reinforces the scientific rigor of cementum-based age estimation as a reliable adjunct in forensic odontology.
References
2. . Stein TJ. “Anatomy of root apex and histological changes with age.” Oral Surg Oral Med Oral Pathol. 1990; 69: 266-70.
3. Tencate A. “The estimation of age of skeletal remains from color of roots of teeth.” J Can Dent Assoc. 1977; 2: 83-6.
4. Gordan BC. “Archeological tooth and bone seasonal increments: the need for standardized terms and techniques.” Archaezoologia. 1993; 2: 9-16.
5. Schröder HE. Oralestrukturbildung: Etwicklungsge-schichte, struktur und function normalerhartgewebe und weichgewebe der mundhöhle und des kiefergelenkes. Berlin: Thieme .Verlag; 2000.
6. Aggarwal P, Saxena S, Bansal P. “Incremental lines in root cementum of human teeth: An approach to their role in age estimation using polarizing microscopy.” Indian J Dent Res. 2008; 19: 326-30.
7. . Kvaal S, Solheim. T. “Incremental lines in human dental cementum in relation to age.” Eur J Oral Sci. 1995; 103: 225-30.
8. SiddharthPundir, SusmitaSaxena, PoojaAggrawal. “Estimation of age based on tooth cementum annulations using three different microscopic methods.” Journal of Forensic Dental Sciences. 2009; 1(2): 82-7
9. Condon Keith, K Douglas, James M Charles. Cheverud and E Jane Buikstra. “Cementum Annulation and Age Determination in Homo sapiens. Its Estimates and Accuracy.” American Journal of Physical Anthropology. 1986; 71: 321-330.
10. CharanGowda BK, SrinivasaReddy.P, Kokila G, Pradeep L. “Cemental annulation and phase contrast microscope; tool for age estimation.” 2014; 6(1): 9-13.
11. . PriyaShirish Joshi, Madhuri S. Chougule, Gaurav Pralhad Agrawal. “Comparison of polarizing & phase contrast microscopy for estimation of age based on cemental annulations.” 2010; 3(3): 17-25.
12. Avadhani A, Tupkari JV, Khambati A, Sardar M. “Cementum annulations and age determination.” J For Dent Sci. 2009; 1(2): 73-6.
13. I kaur, D Shetty, A Manchanda, H Singh. “Efficacy of Age Estimation In Forensic Dentistry Using Cemental Annulations As A Criteria.” 2009; 4(2): 1-5.
14. Marija Randovic. Ageing in the Danube gorges population (9500-5500 BC) – tooth cementum annulations method. Bioarchaeology of ancient Europe. 2012; 47: 9-
15. Priya Gupta et al., Human Age Estimation from Tooth Cementum and Dentin Journal of Clinical and Diagnostic Research. 2014 Apr, Vol-8(4)
16. Anil Patel et al., Age Estimation Based On Incremental Lines Of Cementum Using Three Different Microscopes, NeuroQuantology|August 2022|Volume20|Issue10|Page 7561-7569.
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Dr. Neha Upadhyaya
PhD Scholar, Department of Oral Pathology and Microbiology NIMS Dental College and Hospital, Jaipur, Rajasthan, India.
Dr. Rohit Sharma
Professor and Head, Department of Oral Pathology and Microbiology, NIMS Dental College and Hospital, Jaipur, Rajasthan, India.
Dr. M.K. Sunil
Principal and Professor, NIMS Dental College and Hospital, Jaipur, Rajasthan, India.
Dr. Rakesh Kumar Mishra
Professor, Department of Otorhinolaryngology, PIMS, Banthara, Lucknow, India.
Dr. Akansha Srivastava
Senior Lecturer, Department of Oral Pathology and Microbiology, Dr B R Ambedkar Dental College, Patna, India.