EVALUATION OF NEPHROPROTECTIVE POTENTIAL OF FICUS RELIGIOSA LEAVES AGAINST GENTAMICIN INDUCED NEPHROTOXICITY IN EXPERIMENTAL ALBINO WISTAR RATS
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Keywords
Ficus religiosa, IAEC, Nephroprotective, GM, phytochemical, biochemical parameters, histopathology, antioxidant activity, HPLC.
Abstract
Ficus religiosa (Peepal) belonging to family Moraceae is one of the most versatile medicinal plants having a wide spectrum of biological activity and traditionally used to treat gonorrhoea, diarrhoea, and dysentery and other urogenital disorders, and kidney disorders. Ficus religiosa contained tannins, phenols, saponins, alkaloids, methionine, terpenoids, flavanoids, glycosides, proteins, essential and volatile oils and steroids. Previous pharmacological studies revealed that Ficus religiosa possessed antimicrobial, anti-amnesic, anticholinergic, antidiabetic, anti-inflammatory, analgesic, cytotoxic, anti-ulcer, wound healing, antioxidant, anti- asthmatic, hepatoprotective effects. The present study was carried out to evaluate the Nephroprotective potential of Ficus religiosa leaves on gentamicin induced nephrotoxicity in Albino Wistar rats.
Material and Methods: The plant material were collected, dried and authenticated from the CSIR-NSCAIR, New Delhi. HPLC studies were also performed. Albino Wistar rats weighing 150-200g of either gender were used in the study, after the approval of IAEC. The albino wistar rats were randomly divided into seven groups of 5 animals in each. Group I was administered normal saline for 14 days. Group I was administered normal saline for 14 days. Gentamicin (GM, 80mg/kg, i.p.) was administered from 8 day and induced till 14 day in Group II to Group VII. 200mg/kg (Group IV and Group VI) and 400mg/kg (Group V and Group VII) ethanolic extract of Ficus mature and young leaves and 500mg/kg (Group III) of cystone as a standard was treated to rats 1 hr. before GM administration. After body weight measurements. The urine of each grpoup of rats was collected and the volume was measured over 24 hrs. On 15 day blood samples were collected and sacrificed. Sera were collected for biochemical analysis (Serun urea, Serum creatinine, uric acid, albumin, albumin, total proteins level and Sodium, Potassium, Chlorides).The animals were sacrificed after anaesthesia. The left kidney was excused for tissues homogenates that were used for biochemical parameters (Malondialdehyde, Superoxide dismutase, Glutathione, Catalase) and right kidney were used for histopathological studies.
Result: The phytochemical and HPLC analysis were measured.Treatment with ethanolic extract of FYL(200mg/kg,400mg/kg) significantly improved the altered levels of Serum Creatinine, Serum Urea , Uric acid, Albumin, Total proteins, Sodium, Potassium and Chlorides as compared to disease control groups. It also significantly restored the altered levels of MDA, SOD, GSH and CAT in renal tissues. Apart from these, extract of Ficus religiosa leaves also diminished histopathological alterations induced by GM in kidney injury .
Conclusion: The result of this study observed that the ethanolic extract of FML and FYLhasa significant nephroprotective effects against gentamicin –induced nephrotoxicityin Albino Wistar rats..
References
2. Tanase, Daniela Maria, Evelina Maria Gosav, Smaranda Radu, Claudia Florida Costea, Manuela Ciocoiu, Alexandru Carauleanu, Cristina Mihaela Lacatusu, Minela Aida Maranduca, Mariana Floria, and Ciprian Rezus. "The predictive role of the biomarker kidney molecule-1 (KIM-1) in acute kidney injury (AKI) cisplatin-induced nephrotoxicity." International journal of molecular sciences 20, no. 20 (2019): 5238.
3. Kellum, J.A.; Lameire, N.; Aspelin, P.; Barsoum, R.S.; Burdmann, E.A.; Goldstein, S.L.; Herzog, C.A.; Joannidis, M.; Kribben, A.; Levey, A.S.; et al. Kidney Disease: Improving Global Outcomes (KDIGO) Acute Kidney Injury Work Group. KDIGO clinical practice guideline for acute kidney injury. Kidney Int. Suppl. 2012, 2, 1–138. [Google Scholar]
4. Murray, P.T.; Mehta, R.L.; Shaw, A.; Ronco, C.; Endre, Z.; Kellum, J.A.; Chawla, L.S.; Cruz, D.; Ince, C.; Okusa, M.D. Potential use of biomarkers in acute kidney injury: Report and summary of recommendations from the 10th Acute Dialysis Quality Initiative consensus conference. Kidney Int. 2014, 85, 513–521. [Google Scholar] [CrossRef]
5. Kwiatkowska, Ewa, Leszek Domański, Violetta Dziedziejko, Anna Kajdy, Katarzyna Stefańska, and Sebastian Kwiatkowski. "The mechanism of drug nephrotoxicity and the methods for preventing kidney damage." International Journal of Molecular Sciences 22, no. 11 (2021): 6109.
6. Perazella, M.A. Pharmacology behind Common Drug Nephrotoxicities. Clin. J. Am. Soc. Nephrol. 2018, 13, 1897–1908. [Google Scholar] [CrossRef]
7. Luque, Y.; Louis, K.; Jouanneau, C.; Placier, S.; Esteve, E.; Bazin, D.; Rondeau, E.; Letavernier, E.; Wolfromm, A.; Gosset, C.; et al. Vancomycin-Associated Cast Nephropathy. J. Am. Soc. Nephrol. 2017, 28, 1723–1728. A.I. Morales et al. Metformin prevents experimental gentamicin-induced nephropathy by a mitochondria-dependent pathway Int. (2010).
8. P. Balakumar et al. Emerging role of PPAR ligands in the management of diabetic nephropathy Pharmacol Res(2009).
9. S.A. Khan et al. Protective effect of green tea extract on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney Pharmacol Res (2009).
10. Valibeik, Ali, Negar Naderi, Abdolhakim Amini, Niloufar Tavakoli Dastjerd, Sobhan Rahimi Monfared, Leila Jafaripour, Saeed Veiskarami, Mehdi Birjandi, Alireza Naderi Sohi, and Hassan Ahmadvand. "Effect of camphor on biochemical factors and gene expression of antioxidant enzymes, inflammatory and apoptotic factors against gentamicin-induced nephrotoxicity in rats." Journal of Renal Injury Prevention 10, no. 3 (2020): e21-e21.
11. Ghadigaonkar, Sushma, A. G. Reddy, B. Kalakumar, M. Lakshman, and U. Rajkumar. "Quantification of total phenolic content, total flavonoid content and evaluation of in vitro free radical scavenging activities in Ficus religiosa Linn." Pharm Innov J 10, no. 3 (2021): 84-8.
12. ARCHANA, VAJPAYEE, DWIVEDI SUMEET DUBEY KUSHAGRA, and JOSHI HEMANT. "4. COMPARATIVE STANDARDIZATION PARAMETERS OF FICUS CARICA LINN. AND FICUS RELIGIOSA LINN. BY VAJPAYEE ARCHANA1, DWIVEDI SUMEET, DUBEY KUSHAGRA AND JOSHI HEMANT." LIFE SCIENCES LEAFLETS 12 (2011): 376-383.
13. Majumder Pulak et al, An ethano-phytochemical and pharmacological review on novel indian medicinal plants used in herbal formulations, International Journal of Pharmacy and Pharmaceutical Sciences, 2013, Vol. 5(4): 74-83
14. Soni Rupesh et al, Effect of ethanolic extract of cinnamomum tamala leaves on wound healing in stz induced diabetes in rats, Asian J Pharm Clin Res, 2013, Vol 6 (4): 39-42
15. Satyal Prabodh et al, Bioactivities and Compositional Analyses of Cinnamomum Essential Oils from Nepal: C. camphora, C. tamala, and C. glaucescens , Natural Product Communications, 2013, Vol. 8 (12):1777-1784.
16. BALIGA, RADHAKRISHNA, NORISHI UEDA, PATRICK D. WALKER, and SUDHIR V. SHAH. "Oxidant mechanisms in toxic acute renal failure." Drug metabolism reviews 31, no. 4 (1999): 971-997
17. Randjelovic, Pavle, Slavimir Veljkovic, Nenad Stojiljkovic, Ljubinka Velickovic, Dusan Sokolovic, Milan Stoiljkovic, and Ivan Ilic. "Protective effect of selenium on gentamicin-induced oxidative stress and nephrotoxicity in rats." Drug and chemical toxicology 35, no. 2 (2012): 141-148.
18. Son, Yong, Sangduck Kim, Hun-Taeg Chung, and Hyun-Ock Pae. "Reactive oxygen species in the activation of MAP kinases." Methods in enzymology 528 (2013): 27-48.
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Mrs. Jaya Upadhyay
Assistant Professor Kailash Institute of Pharmacy & Management GIDA, Gorakhpur
Dr.Dharamveer panjwani
Head of Department, Hygia Institute of Pharmaceutical Education & Research, Lucknow
Dr. Abhishek Gupta
Associate Professor, Hygia Institute of Pharmaceutical Education & Research
Dr.J.N Mishra
Director, Kailash Institute of Pharmacy & Management GIDA Gorakhpur