STRUCTURAL BIOINFORMATICS OF HAEMOPHILUS INFLUENZAE MACRODOMAIN TER PROTEIN (MATP): IMPLICATIONS IN BIOCHEMISTRY, HEMATOLOGY AND DRUG DEVELOPMENT
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Keywords
MatP protein, HI1647 gene, Macrodomain Ter, Chromosome segregation, Homology modeling
Abstract
Introduction: Haemophilus influenzae is a significant pathogen implicated in respiratory and systemic infections, particularly among vulnerable populations. The MatP protein (HI1647 gene product) plays a critical role in chromosome segregation and bacterial viability, making it a potential therapeutic target. This study aimed to model the 3D structure of the MatP protein and correlate its relevance with clinical and hematological parameters in infected patients.
Objective: To model the three-dimensional structure of the structurally conserved MatP protein (HI1647) of Haemophilus influenzae using homology modeling and to investigate its potential as a novel antimicrobial drug target by correlating its role in bacterial viability with clinical and hematological indicators of infection severity in affected patients.
Methodology: The MatP amino acid sequence was retrieved from NCBI, and a BLASTp search was performed against the PDB. The E. coli MatP/MatS complex (PDB ID: 3VEA) was selected as a template for homology modeling using MODELLER 9.10. The best model was validated using PROCHECK. Clinical and laboratory data, including CRP, ALT, LDH, IL-6, WBC count, neutrophils, platelets, and hemoglobin levels, were collected from confirmed H. influenzae cases.
Results: The modeled HI1647 MatP protein showed high structural quality with >90% residues in the most favored regions of the Ramachandran plot. Clinically, infected patients exhibited elevated CRP, IL-6, and neutrophil levels, along with altered platelet counts.
Conclusion: The study confirms the structural conservation of the MatP protein and highlights its potential as a drug target. Hematological parameters provide valuable indicators of infection severity, supporting an integrated approach to diagnosis and therapeutic development in H. influenzae infections.
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Dr. Halima Sadi
Assistant Professor of Pharmacology, Department of Pharmacology, Bacha Khan Medical College, Mardan, Pakistan
Dr. Naeema Afzal
Professor of Pathology Department, Abbottabad International Medical College, Abbottabad, Pakistan
Dr. Hafiz Sajid Akbar
Associate Professor, Vertex Institute of Pharmacy, Mardan, Pakistan
Dr. Sarwat Abbasi
Assistant Professor, Biochemistry, Ayub Medical College, Abbottabad, Pakistan
Dr. Anber Noreen
Lecturer of Pathology Department, College of Medicine and Dentistry, Abbottabad, Pakistan
Dr. Muhammad Kaleem Khan
Associate Professor of Haematology, Women Medical College/District Pathologist, DHQ Teaching Hospital, Abbottabad, Pakistan