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Zainab Thaer Hameed
Seyyed Meysam Abtahi Froushani


lncRNA MEG-3, Gene expression, oral squamous cell carcinoma, biomarker, Neoplasm


Background: According to previous studies, aberrant expression of various non-coding RNAs, including microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), plays undeniable roles in the development and progression of different types of cancer, including OSCC. This study aimed to investigate long non-coding RNA Maternally Expressed 3 (LNC- MEG3) expression patterns as a potential diagnostic biomarker for oral squamous cell carcinoma (OSCC).

Method: In this cross-sectional study, LNC- MEG3 expression patterns were examined using real-time PCR method. Firstly, tumor tissue and margins were separated during surgery in 100 patients. Then total RNA was extracted and transcribed to cDNA from the obtained to measure the transcription level of lnc-MEG3 by qRT-PCR. GraphPad 6 Prism v.8 was used to perform the statistical analyses. Comparisons between groups in internal samples were conducted by paired student's t-test (P < 0.05). 

Results: Our results indicated that the lncRNA MEG3 is downregulated in oral squamous cell carcinoma (OSCC) tumor samples compared to adjacent marginal tissue. These changes were significantly associated with lymph node invasion, tissue differentiation, and metastasis. Also, using receiver operating characteristic curve analysis, the MEG-3 expression pattern was found as a possible diagnostic biomarker for OSCC (The area under the curve was 0.62 and p-value:0.012).

Conclusion: We identified lncRNA MEG-3 downregulation in OSCC tumorigenesis. Our findings suggest lncRNA MEG-3 as a possible biomarker for OSCC.

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