EVALUATION OF EXPRESSION OF MIR-21 AND MIR-548 IN ORAL SQUAMOUS CELL CARCINOMA (OSCC)

Main Article Content

Zainab Thaer Hameed
Seyyed Meysam Abtahi Froushani

Keywords

miR-21, miR-548, Gene expression, oral squamous cell carcinoma, biomarker, micro-RNA

Abstract

Background: microRNAs (miRNAs) are a group of short non-coding RNAs with 18-22 bp length, this category of genes plays critical roles in cellular pathways and dysregulation of their expression is related to the development and progression of different types of cancer including OSCC. This study evaluated miR-21 and miR-548 expression patterns in OSCC samples and these genes' potential as diagnostic biomarkers for oral squamous cell carcinoma (OSCC).


Method: In this cross-sectional study, tumor and marginal tissues were collected from 100 patients with OSCC. Following RNA extraction, miR-21 and miR-548 expression levels were measured using SYBR green master mix and real-time quantitative PCR.


Results: Our study revealed a notable rise in miR-21 and miR-548 expression levels in tumor tissue samples compared to marginal tissue samples. Additionally, miRNA expression was linked to specific clinicopathological features of the patients. ROC curve analysis indicates that only the expression of miR-21 can be used as a biomarker for OSCC.


Conclusions: Our data showed upregulation of miR-21 and miR-548. However, based on our findings, only miR-21 expression could serve as a promising diagnostic and prognostic biomarker in OSCC. Further research is necessary to validate this assertion.

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