SIMULTANEOUS QUANTIFICATION OF FLAVONOIDS BY USING RP-HPLC, PHYTOCHEMICAL PROFILING AND CYTOTOXIC ACTIVITY OF HIPPOPHAE RHAMNOIDES BERRIES.

Main Article Content

Sahar Zahid
Sadia Chaman
Shaista Qamar
Rabia saeed
Fatima Usman
Hamza Imam Dar
Ahmad Asghar
Muhammad Ahad Ali
Ali Abbas
Ali Abbas
Toobah Ashraf

Keywords

Hippophae, Phytochemicals, HPLC, flavonoids, cytotoxicity

Abstract

Hippophae rhamnoides commonly known as Seabuck thorn is native to Europe and Asia. This plant has been reported for the treatment of pulmonary, gastrointestinal, cardiac and blood disorders. The present study, for the first time, was undertaken to explore the biological and phytochemical profiling of the methanol, ethanol and aqueous extracts of berries of H. rhamnoides . Phytochemical analysis revealed the presence of alkaloids, glycosides, tannins, flavonoids and terpenoids. Proximate analysis i.e. moisture content, pH and ash values were in normal range indicating the stability of crude powder. Elemental analysis indicated the presence of essential mineral nutrients in trace amounts. RP-HPLC based quantification revealed the presence of significant amount of flavonoids ranging from 0.005 - 5.450 µg/ml. The plant exhibited considerable levels of cytotoxicity against BHK-21 cell line with the LC50 values ranging between 75-330 µg/ml, while extracts at concentration of 400 μg/ml showed significant cytotoxicity for Vero cell line.

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