ASSESSMENT OF WOUND HEALING ACTIVITY OF A TOPICAL HERBAL GEL IN AN EXCISION MODEL OF ALBINO WISTAR RATS
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Keywords
Topical Herbal Gel, Excision Model, Wound Contraction, Interleukin-1-beta, Transforming Growth Factor-beta1
Abstract
There are several promising preclinical models, including mice, rats, rabbits, and pigs, that can be utilized to initiate acute or chronic wounds. These models can be induced using various distinct techniques, with excision being the most common. Once a suitable model has been determined for a study, researchers must select an appropriate and reproducible technique that enables the monitoring of wound improvement over time. This study analyzed the healing efficacy of ATC Topical herbal gel in Wistar rats using the excision wound model. The ATC Topical herbal gel
was prepared at concentrations of 1%, 3%, and 9%, respectively, along with standard drugs (Povidone-Iodine Ointment USP 5% w/w-PI). The wound area contraction was measured on the 1st, 5th, 15th, and 21st days. The treated group exhibited significant changes in the improvement of wound healing and epithelialization, as indicated by biochemical biomarkers such as SOD (Superoxide dismutases), GSH (Reduced Glutathione), CAT (catalase), and MPO (myeloperoxidase). Furthermore, the treated group demonstrated a well-organized dermis devoid of inflammatory cells compared to the disease control group. Additionally, this experiment aimed to examine cytokine levels following wound healing.
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Dinesh Kumar
Research Scholar, Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan
Vivek
Associate Professor, Shri Jagdishprasad Jhabarmal Tibrewala University, Jhunjhunu, Rajasthan,
Rakesh Kumar Jat
Principal, Institute of Pharmacy, Shri Jagdishprasad Jhabarmal, Tibrewala University, Jhunjhunu, Rajasthan