Anticariogenic Anti Inflammatory and Cytotoxic Effects of Mouthwash Prepared Using Oolong Tea and Triphala Formulation

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Lakshana Suresh
Sandhya Sundar
Rajesh Kumar Shanmugam
Ramya Ramadoss
Suganya Paneerselvam
Pratibha Ramani


It can be concluded that the mouthwash prepared using oolong tea and triphala show strong anti cariogenic, anti inflammatory and cytotoxic effects.


Background: Oolong tea has remarkable health benefits despite it not being the most well-liked supplement. It can help with blood sugar management and weight loss, as well as prevent cancer, cardiovascular disease, and age-related cognitive decline. It could play a part in preventing cancer. Since ancient times, triphala has been utilized in traditional Ayurvedic medicine as a multipurpose remedy for symptoms ranging from stomach aches to tooth decay.
Aim: To assess the anticariogenic, anti-inflammatory and cytotoxic effects of mouthwash prepared using Oolong tea and Triphala formulation.
Materials and methods: An in vitro study was conducted comparing standard mouthwash with the mouthwash prepared using oolong tea and triphala formulation.The anti-inflammatory activity of the mouthwash was determined by EA assay and BSA assay and anti- cariogenic property was determined by zone of inhibition. The cytotoxicity was determined by brine shrimp lethality assay.
Results: The results showed that as the concentration of the mouthwash increases the percentage of inhibition. The cytotoxicity was determined by testing the mouthwash on brine shrimps.The various concentrations were 5,10,20,40 and 80 micrometers.The values noted were 8,7,7,6 and 5 respectively. The number of live nauplii present were calculated by using the following formula. Number of dead nauplii/number of dead nauplii+number of live nauplii x100.

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