Evaluation cytogenotoxicity of cobalt oxide nanoparticle in mice liver
Main Article Content
Keywords
Cobalt oxide nanoparticles, Cytogenotoxicity, Comet assay, Liver
Abstract
This study was done on 4 groups of mice to evaluate the cytogenotoxicity of cobalt oxide nanoparticles by comet assay from liver tissue and bone marrow to detect DNA damage and the histopathological changes in the hepatic tissues. Significant elevation in %DNA, tail length, and tail moment in liver while in bone marrow the %DNA, tail length, and tail moment showed elevation in 5% cobalt oxide nanoparticles and decline in 10% and 20% of cobalt oxide nanoparticles. The liver tissues of the 5% cobalt nanoparticle group had areas of hepatocellular necrosis, focal aggregations of mononuclear inflammatory cells, and portal congestion, whereas the 10% cobalt oxide nanoparticle group had marked inflammatory cell infiltration, marked vascular congestion, and small focal areas of hepatocellular necrosis that were infiltrated by mononuclear inflammatory cells. The hepatic tissue parenchyma in the 20% cobalt oxide nanoparticles group had clogged blood arteries, and regions of hepatocellular necrosis with mononuclear inflammatory cell infiltration were often seen lesions in the liver. Some liver tissues, however, showed only little improvement. In conclusion the cobalt oxide nanoparticles were showed cytotoxicity and genotoxicity especially in concentration of 5% and 10%.
References
1. Giri AK, Pellerin K, Pongsaksa W, Sorescu M, Majetich SA, Effect of light on the magnetic properties of cobalt ferrite nanoparticles. IEEE Transactions on Magnetics, 2000;36 (5):3029-31.
2. Barillet S , Jugan ML, Laye M, Leconte Y, Herlin-Boime N, Reynaud C, Carrière M, In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-genotoxicity and oxidative stress. Toxicol Lett, 2010; 07.009. Epub.
3. Kim IY, Joachim E, Choi H, Kim K, Toxicity of silica nanoparticles depends on size, dose, and cell type. Nano medicine, 2015;11(6):1407–16.
4. Moradpoor H, Safaei M, Rezaei F, Golshah A, Jamshidy L, Hatam R, Abdullah RS, Optimisation of cobalt oxide nanoparticles synthesis as bactericidal agents. Open Access Maced. J. Med. Sci, 2019;7:2757–2762.
5. Rabani I, Yoo J, Kim HS, Lam DV, Hussain S, Karuppasamy K, Seo YS, Highly dispersive Co3O4 nanoparticles incorporated into a cellulose nanofiber for a high-performance flexible super
capacitor, Nanoscale, 2021;13:355–370.
6. Khan I, Saeed K, Khan I, Nanoparticles: properties, applications and toxicities, Arab J Chem, 2019;12(7):908–31.
7. Nadeem M, Khan R, Afridi K, Nadhman A, Ullah S, Faisal S, Mabood ZU, Hano C, Abbasi B H, Green synthesis of cerium oxide nanoparticles (CeO2 NPs) and their antimicrobial applications: a review, Int J Nanomed, 2020; 15: 5951.
8. Faucon, M P, Pourret O, Lange B, Element case studies: cobalt and copper. In: Agromining: farming for metals. Cham, Springer, 2018; 233–9.
9. Iravani S, Varma R S, Sustainable synthesis of cobalt and cobalt oxide nanoparticles and their catalytic and biomedical applications, Green Chem, 2020; 22 (9): 2643–61.
10. Demir E, Creus A, Marcos R, Genotoxicity and DNA Repair Processes of Zinc Oxide Nanoparticles, J Toxicol. Environ, ;2014,77 (21): 1292–1303.
11. Elena Bossi, Daniele Zanella, Rosalba Gornati, Giovanni Bernardini, Cobalt oxide nanoparticles can enter inside the cells by crossing plasma Membranes, Scientific Reports; 2016, 6(1):22254
12. Moulton MC, Braydich-Stolle LK, Nadagouda MN, Kunzelman S, Hussain SM, Varma R S, Synthesis, characterization and biocompatibility of “green” synthesized silver nanoparticles using tea polyphenols, Nanoscale 2010; 2 :763–770.
13. Warheit DB, Laurence BR, Reed KL, Roach DH, Reynolds GA, Webb TR, Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. Toxicol. Sci, 2004; 77: 117–125.
14. Cheng R, Feng F, Meng F, Deng C, Feijen J, Zhong Z, Glutathione-responsive nano-vehicles as a promising platform for targeted intracellular drug and gene delivery, J. Control. Release, 2011; 152: 2–12.
15. Hussain S, Hess K, Gearhart J, Geiss K, Schlager J, In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol. In Vitro 2005; 19: 975–983.
16. Xia T, Kovochich M, Brant J, Hotze M, Sempf J, Oberley T, Sioutas C, Yeh JI,Wiesner MR, Nel AE, Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett, 2006; 6: 1794–1807.
17. Gualtierotti R, Guarnaccia L, Beretta M, Navone SE, Campanella R, Riboni L, Rampini P, Marfia G, Modulation of neuro inflammation in the central nervous system: Role of chemokines and sphingolipids. Adv. Ther. 2017; 34: 396–420.
18. Hornos Carneiro MF, Barbosa F Jr, Gold nanoparticles: A critical review of therapeutic applications and toxicological aspects. J. Toxic. Environ. Health, 2016; 19:129–148
19. You Y, He L, Ma B, Chen T, High-drug-loading mesoporous silica nanorods with reduced toxicity for precise cancer therapy against nasopharyngeal carcinoma. Adv. Funct. Mater. 2017; 27: 1703313.
20. Monteiller C, Tran L, MacNee W, Faux S, Jones A, Miller B, Donaldson K, The pro-inflammatory effects of low-toxicity low-solubility particles, nanoparticles and fine particles, on epithelial cells in vitro: the role of surface area, Occup. Environ. Med, (2007) ;64: 609–615.
21. Papageorgiou I, Brown C, , Schins R, Singh S, Newson R, Davis S, Fisher J, Ingham E, Case CP, The effect of nano- and micron-sized particles of cobalt–chromium alloy on human fibroblasts in vitro, Biomaterials 2007; 28: 2946–2958.
22. Ponti J, Sabbioni E, Munaro B, Broggi F, Marmorato P, Franchini F, Colognato R, Rossi F, Genotoxicity and morphological transformation induced by cobalt nanoparticles and cobalt chloride: an in vitro study in Balb/3T3 mouse fibroblasts. Mutagenesis, 2009 ;24(5):439-45.
23. Peters K, Unger RE, Kirkpatrick CJ, Gatti AM, Monari E, Effects of nanoscaled particles on endothelial cell function in vitro: studies on viability, proliferation and inflammation, J. Mater. Sci. Mater. Med, 2004; 15: 321–325.
24. Petrarca C, Perrone A, Verna N, Verginelli F, Ponti J, Sabbioni E, Cobalt nanoparticles modulate cytokine in vitro release by human mononuclear cells mimicking autoimmune disease, Int. J. Immunopathol. Pharmacol, (2006);19: 11–14.
25. Mo Y, Xinqiang Z, Xiao H, Tollerud J, Qunwei Z, Cytokine and NO release from peripheral blood neutrophils after exposure to metal nanoparticles: in vitro and ex vivo studies .
Nanotoxicology 2(2):79-87
26. Magaye R, Zhao J, Bowman L, Ding M, Genotoxicity and carcinogenicity of cobalt-, nickel- and copper-based nanoparticles. Exp Ther Med. 2012; 4(4):551–561.
27. Uboldi C, Orsière T, Darolles C, Aloin V, Tassistro V,George I, Malard V, Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways. Part Fibre. 2016;13(5):1–15.
28. Alarifi S, Ali D, Ao Y, Ahamed M, Siddiqui MA, Al-Khedhairy AA, Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells. Int J Nanomedicine. 2013; 8:189–199.
29. Cavallo D, Ciervo A, Fresegna AM, Maiello R, Tassone P, Buresti G, Casciardi S, Iavicoli S, Ursini CL, Investigation on cobalt-oxide nanoparticles cyto-genotoxicity and inflammatory response in two types of respiratory cells. J Appl Toxicol. 2015; 35:1102–1113.
30. Bucher JR, Hailey JR, Roycroft JR, Haseman JR, Sills RC, Grumbein SL, Mellick PW, Chou BJ, Inhalation toxicity and carcinogenicity studies of cobalt sulfate. Toxicol Sci. 1999; 49:56–67.
31. Chattopadhyay S, Dash SK, Tripathy S, Das B, Mandal D,Pramanik P, Roy S, Toxicity of cobalt oxide nanoparticles to normal cells; an in vitro and in vivo study. Chem Biol Interact. 2015; 25(226):58–71.
32. Rahimi-Nasrabadi M, Naderi H R, Karimi M S, Ahmadi F, Pourmortazavi SM, Cobalt carbonate and cobalt oxide nanoparticles synthesis, characterization and supercapacitive evaluation. J Mater Sci: Mater Electron 2017; 28: 1877–1888.
33. Tice R R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H , Sasaki Y F, Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and molecular mutagenesis, 2000; 35(3), 206-221.
34. Bancroft J D, Layton C, The hematoxylin and eosin, connective and mesenchymal tissues with their stains,” in Bancroft s Theory and Practice of Histological Techniques, eds K. S. Suvarna, C. Layton, and J. D. Bancroft (Philadelphia, PA: Churchill Livingstone), 2013; 173–186.
35. Spigoni V, Cito M, Alinovi R, et al, Effects of TiO2 and Co3O4 nanoparticles on circulating angiogenic cells. PLoS One (2015); 10(3): e0119310.
36. Chattopadhyay S, Dash SK, Tripathy S, et al, Cobalt oxide nanoparticles induced oxidative stress linked to activation of TNF-_/caspase-8/p38-MAPK signaling in human leukemia cells. Journal of Applied Toxicology 2015b; 35: 603–613.
37. Abudayyak M, Tuba Altincekic Gurkaynak, Gul O¨ zhan, In vitro evaluation of cobalt oxide nanoparticle-induced toxicity. Toxicology and Industrial Health, 2017; Vol. 33(8): 646–654
38. Liu Y K, XX Deng, HL Yang, Cytotoxicity and genotoxicity in liver cells induced by cobalt nanoparticles and ions . BONE & JOINT RESEARCH vol. 5, No. 10, October 2016
39. Ali AA , Mohamed HRH, Genotoxicity and oxidative stress induced by the orally administered nanosized nickel and cobalt oxides in male albino rats. The Journal of Basic and Applied Zoology, (2019); 80:2
40. Mohamed H R H, Hussien N A, Amelioration of cobalt oxide nanoparticles induced genomic and mitochondrial DNA damage and oxidative stress by omega-3 co-administration in mice. Caryologia, (2018); 71(4): 357– 364
41. Rowe L A, Degtyareva N, Doetsch P W, DNA damage-induced reactive oxygen species (ROS) stress response in Saccharomyces cerevisiae. Free Radical Biology and Medicine, (2008); 45(8): 1167–1177
42. Kang M A, So E Y, Simons A L, Spitz D R, Ouchi, T, DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway. Cell Death and Disease, (2012); 3(1), e249.
43. Gerloff K, Fenoglio I, Carella E, Kolling J, Albrecht C, Boots AW, Förster I, Schins RP, Distinctive toxicity of TiO2 rutile/anatase mixed phase nanoparticles on Caco-2 cells. Chem Res Toxicol, 2012;25:646–655.
44. Magdolenova Z, Bilanièová D, Pojana G, Fjellsbø LM, Hudecova A, Hasplova K, Marcomini A, Dusinska M, Impact of agglomeration and different dispersions of titanium dioxide nanoparticles on the human related in vitro cytotoxicity and genotoxicity. J Environ Monitor, 2012;14:455–464
2. Barillet S , Jugan ML, Laye M, Leconte Y, Herlin-Boime N, Reynaud C, Carrière M, In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: cyto-genotoxicity and oxidative stress. Toxicol Lett, 2010; 07.009. Epub.
3. Kim IY, Joachim E, Choi H, Kim K, Toxicity of silica nanoparticles depends on size, dose, and cell type. Nano medicine, 2015;11(6):1407–16.
4. Moradpoor H, Safaei M, Rezaei F, Golshah A, Jamshidy L, Hatam R, Abdullah RS, Optimisation of cobalt oxide nanoparticles synthesis as bactericidal agents. Open Access Maced. J. Med. Sci, 2019;7:2757–2762.
5. Rabani I, Yoo J, Kim HS, Lam DV, Hussain S, Karuppasamy K, Seo YS, Highly dispersive Co3O4 nanoparticles incorporated into a cellulose nanofiber for a high-performance flexible super
capacitor, Nanoscale, 2021;13:355–370.
6. Khan I, Saeed K, Khan I, Nanoparticles: properties, applications and toxicities, Arab J Chem, 2019;12(7):908–31.
7. Nadeem M, Khan R, Afridi K, Nadhman A, Ullah S, Faisal S, Mabood ZU, Hano C, Abbasi B H, Green synthesis of cerium oxide nanoparticles (CeO2 NPs) and their antimicrobial applications: a review, Int J Nanomed, 2020; 15: 5951.
8. Faucon, M P, Pourret O, Lange B, Element case studies: cobalt and copper. In: Agromining: farming for metals. Cham, Springer, 2018; 233–9.
9. Iravani S, Varma R S, Sustainable synthesis of cobalt and cobalt oxide nanoparticles and their catalytic and biomedical applications, Green Chem, 2020; 22 (9): 2643–61.
10. Demir E, Creus A, Marcos R, Genotoxicity and DNA Repair Processes of Zinc Oxide Nanoparticles, J Toxicol. Environ, ;2014,77 (21): 1292–1303.
11. Elena Bossi, Daniele Zanella, Rosalba Gornati, Giovanni Bernardini, Cobalt oxide nanoparticles can enter inside the cells by crossing plasma Membranes, Scientific Reports; 2016, 6(1):22254
12. Moulton MC, Braydich-Stolle LK, Nadagouda MN, Kunzelman S, Hussain SM, Varma R S, Synthesis, characterization and biocompatibility of “green” synthesized silver nanoparticles using tea polyphenols, Nanoscale 2010; 2 :763–770.
13. Warheit DB, Laurence BR, Reed KL, Roach DH, Reynolds GA, Webb TR, Comparative pulmonary toxicity assessment of single-wall carbon nanotubes in rats. Toxicol. Sci, 2004; 77: 117–125.
14. Cheng R, Feng F, Meng F, Deng C, Feijen J, Zhong Z, Glutathione-responsive nano-vehicles as a promising platform for targeted intracellular drug and gene delivery, J. Control. Release, 2011; 152: 2–12.
15. Hussain S, Hess K, Gearhart J, Geiss K, Schlager J, In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicol. In Vitro 2005; 19: 975–983.
16. Xia T, Kovochich M, Brant J, Hotze M, Sempf J, Oberley T, Sioutas C, Yeh JI,Wiesner MR, Nel AE, Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett, 2006; 6: 1794–1807.
17. Gualtierotti R, Guarnaccia L, Beretta M, Navone SE, Campanella R, Riboni L, Rampini P, Marfia G, Modulation of neuro inflammation in the central nervous system: Role of chemokines and sphingolipids. Adv. Ther. 2017; 34: 396–420.
18. Hornos Carneiro MF, Barbosa F Jr, Gold nanoparticles: A critical review of therapeutic applications and toxicological aspects. J. Toxic. Environ. Health, 2016; 19:129–148
19. You Y, He L, Ma B, Chen T, High-drug-loading mesoporous silica nanorods with reduced toxicity for precise cancer therapy against nasopharyngeal carcinoma. Adv. Funct. Mater. 2017; 27: 1703313.
20. Monteiller C, Tran L, MacNee W, Faux S, Jones A, Miller B, Donaldson K, The pro-inflammatory effects of low-toxicity low-solubility particles, nanoparticles and fine particles, on epithelial cells in vitro: the role of surface area, Occup. Environ. Med, (2007) ;64: 609–615.
21. Papageorgiou I, Brown C, , Schins R, Singh S, Newson R, Davis S, Fisher J, Ingham E, Case CP, The effect of nano- and micron-sized particles of cobalt–chromium alloy on human fibroblasts in vitro, Biomaterials 2007; 28: 2946–2958.
22. Ponti J, Sabbioni E, Munaro B, Broggi F, Marmorato P, Franchini F, Colognato R, Rossi F, Genotoxicity and morphological transformation induced by cobalt nanoparticles and cobalt chloride: an in vitro study in Balb/3T3 mouse fibroblasts. Mutagenesis, 2009 ;24(5):439-45.
23. Peters K, Unger RE, Kirkpatrick CJ, Gatti AM, Monari E, Effects of nanoscaled particles on endothelial cell function in vitro: studies on viability, proliferation and inflammation, J. Mater. Sci. Mater. Med, 2004; 15: 321–325.
24. Petrarca C, Perrone A, Verna N, Verginelli F, Ponti J, Sabbioni E, Cobalt nanoparticles modulate cytokine in vitro release by human mononuclear cells mimicking autoimmune disease, Int. J. Immunopathol. Pharmacol, (2006);19: 11–14.
25. Mo Y, Xinqiang Z, Xiao H, Tollerud J, Qunwei Z, Cytokine and NO release from peripheral blood neutrophils after exposure to metal nanoparticles: in vitro and ex vivo studies .
Nanotoxicology 2(2):79-87
26. Magaye R, Zhao J, Bowman L, Ding M, Genotoxicity and carcinogenicity of cobalt-, nickel- and copper-based nanoparticles. Exp Ther Med. 2012; 4(4):551–561.
27. Uboldi C, Orsière T, Darolles C, Aloin V, Tassistro V,George I, Malard V, Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways. Part Fibre. 2016;13(5):1–15.
28. Alarifi S, Ali D, Ao Y, Ahamed M, Siddiqui MA, Al-Khedhairy AA, Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells. Int J Nanomedicine. 2013; 8:189–199.
29. Cavallo D, Ciervo A, Fresegna AM, Maiello R, Tassone P, Buresti G, Casciardi S, Iavicoli S, Ursini CL, Investigation on cobalt-oxide nanoparticles cyto-genotoxicity and inflammatory response in two types of respiratory cells. J Appl Toxicol. 2015; 35:1102–1113.
30. Bucher JR, Hailey JR, Roycroft JR, Haseman JR, Sills RC, Grumbein SL, Mellick PW, Chou BJ, Inhalation toxicity and carcinogenicity studies of cobalt sulfate. Toxicol Sci. 1999; 49:56–67.
31. Chattopadhyay S, Dash SK, Tripathy S, Das B, Mandal D,Pramanik P, Roy S, Toxicity of cobalt oxide nanoparticles to normal cells; an in vitro and in vivo study. Chem Biol Interact. 2015; 25(226):58–71.
32. Rahimi-Nasrabadi M, Naderi H R, Karimi M S, Ahmadi F, Pourmortazavi SM, Cobalt carbonate and cobalt oxide nanoparticles synthesis, characterization and supercapacitive evaluation. J Mater Sci: Mater Electron 2017; 28: 1877–1888.
33. Tice R R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H , Sasaki Y F, Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and molecular mutagenesis, 2000; 35(3), 206-221.
34. Bancroft J D, Layton C, The hematoxylin and eosin, connective and mesenchymal tissues with their stains,” in Bancroft s Theory and Practice of Histological Techniques, eds K. S. Suvarna, C. Layton, and J. D. Bancroft (Philadelphia, PA: Churchill Livingstone), 2013; 173–186.
35. Spigoni V, Cito M, Alinovi R, et al, Effects of TiO2 and Co3O4 nanoparticles on circulating angiogenic cells. PLoS One (2015); 10(3): e0119310.
36. Chattopadhyay S, Dash SK, Tripathy S, et al, Cobalt oxide nanoparticles induced oxidative stress linked to activation of TNF-_/caspase-8/p38-MAPK signaling in human leukemia cells. Journal of Applied Toxicology 2015b; 35: 603–613.
37. Abudayyak M, Tuba Altincekic Gurkaynak, Gul O¨ zhan, In vitro evaluation of cobalt oxide nanoparticle-induced toxicity. Toxicology and Industrial Health, 2017; Vol. 33(8): 646–654
38. Liu Y K, XX Deng, HL Yang, Cytotoxicity and genotoxicity in liver cells induced by cobalt nanoparticles and ions . BONE & JOINT RESEARCH vol. 5, No. 10, October 2016
39. Ali AA , Mohamed HRH, Genotoxicity and oxidative stress induced by the orally administered nanosized nickel and cobalt oxides in male albino rats. The Journal of Basic and Applied Zoology, (2019); 80:2
40. Mohamed H R H, Hussien N A, Amelioration of cobalt oxide nanoparticles induced genomic and mitochondrial DNA damage and oxidative stress by omega-3 co-administration in mice. Caryologia, (2018); 71(4): 357– 364
41. Rowe L A, Degtyareva N, Doetsch P W, DNA damage-induced reactive oxygen species (ROS) stress response in Saccharomyces cerevisiae. Free Radical Biology and Medicine, (2008); 45(8): 1167–1177
42. Kang M A, So E Y, Simons A L, Spitz D R, Ouchi, T, DNA damage induces reactive oxygen species generation through the H2AX-Nox1/Rac1 pathway. Cell Death and Disease, (2012); 3(1), e249.
43. Gerloff K, Fenoglio I, Carella E, Kolling J, Albrecht C, Boots AW, Förster I, Schins RP, Distinctive toxicity of TiO2 rutile/anatase mixed phase nanoparticles on Caco-2 cells. Chem Res Toxicol, 2012;25:646–655.
44. Magdolenova Z, Bilanièová D, Pojana G, Fjellsbø LM, Hudecova A, Hasplova K, Marcomini A, Dusinska M, Impact of agglomeration and different dispersions of titanium dioxide nanoparticles on the human related in vitro cytotoxicity and genotoxicity. J Environ Monitor, 2012;14:455–464
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May 5, 2023
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Amer.I.M, Baiomy A.A, Mansour A.B, Hanan R.H, Attia H.F, Gehan B. A Youssef, & Ali.S.A. (2023). Evaluation cytogenotoxicity of cobalt oxide nanoparticle in mice liver. Journal of Population Therapeutics and Clinical Pharmacology, 30(9), 187–197. https://doi.org/10.47750/jptcp.2023.30.09.020
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