Experimental therapeutic challenge of two bacteriophages isolates against E. coli serogroup causing diahrea

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Nidham M. Jamalludeen
Dania M. Shakir
Mariem N Mohammed-Ali


phage therapy, E. coli, experimental trails, Guinea pigs


The study aimed to determine the efficacy of selected bacteriophages in the treatment of experimental diarrhea in a group of laboratory animals infected with Escherichia coli used as challenge bacteria. Bacteriophage was administered orally, up to three times, 24 hours after administration of challenge bacteria, and following diarrhea. Weight change, diarrhea duration, diarrhea severity, diarrhea degree score, and the rate of challenged E. coli bacteria shedding over the course of six days were the parameters utilized to determine if changes had occurred. The bacteriophages that were tested for treatment were successful in making a qualitative change in these parameters. These bacteriophages produced specific changes in all parameters without affecting the normal flora shed in the feces. These results indicate that the selected bacteriophages had an effective effect in treating experimental diarrhea in laboratory animals after giving a challenge bacterium orally.

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