Assessment Of Serum Levels of Zeb2 and Fat10 and Tumor Necrosis Factor-Alpha as Potential Novel Biomarkers in Breast Cancer Patients

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Abdalnaser K. A. Al-Zahrawi
Firas Faris Rija


Five Minute Preceptor, critical thinking, nursing profession students


Background: Breast cancer is the primary and most frequent type of cancer that kills women, ranking fifth among all malignancies that result in fatalities globally and second behind lung cancer in terms of prevalence.
Objective: In this study, zeb 2 and fat10 proteins and pro-inflammatory cytokines (TNF-α) were evaluated in Iraqi women with breast cancer.
Patients and methods: In this study, serum concentrations of zeb 2 ,fat 10 and TNF-α were determined using samples from 90 Iraqi women (60 patients and 30 healthy volunteers) Among them are healthy and patients, premenopausal and postmenopausal woman .
Results: According to the findings of the current study, FAT10 and TNF- α were both considerably elevated in breast cancer patients (P<0.05), and they also had a tight relationship, and ZEB2 was also high on the probability level ( P < 0.01).
Conclusions: This study discovered a link between elevated serum levels of pro-inflammatory cytokines TNF-α, ZEB2, and FAT10

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1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. ( 2018(.Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. Nov;68(6):394-424 .
2. Sung H , ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F.(2020).Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. Nov; 71(3):209-249.
3. Giaquinto AN , Sung H ,Miller, K. D., Kramer, J. L., Newman, L. A., Minihan, A., ... & Siegel, R. L. (2022). Breast cancer statistics, 2022. CA: A Cancer Journal for Clinicians, 72(6), 524-541.
4. Yalaza M , İnan A, Bozer M.) 2016) .Male Breast Cancer. J Breast Health. Jan 1;12(1):1-8.
5. Lawrence, R. A. (2022). Anatomy of the breast. In Breastfeeding (pp. 38-57). Elsevier.
6. Wolczyk, D., Zaremba-Czogalla, M., Hryniewicz-Jankowska, A., Tabola, R., Grabowski, K., Sikorski, A. F., & Augoff, K. (2016). TNF-α promotes breast cancer cell
7. migration and enhances the concentration of membrane-associated proteases in lipid rafts. Cellular Oncology, 39(4), 353-363.
8. Li J, Riedt T, Goossens S, Carrillo García C, Szczepanski S, Brandes M, Pieters T, Dobrosch L, Gütgemann I, Farla N, et al. (2017) The EMT transcription factor Zeb2 controls adult murine hematopoietic differentiation by regulating cytokine signaling. Blood.;129:460–472. 9. Zou Y, Ouyang Q, Wei W, Yang S, Zhang Y, Yang W (2018) .FAT10 promotes the invasion and migration of breast cancer cell through stabilization of ZEB2. Biochem Biophys Res Commun. Nov 30;506(3):563-570.
10. Krishnamoorthy D, Sankaran M. Modulatory effect of Pleurotus ostreatus on oxidant/antioxidant status in 7, 12-dimethylbenz (a) anthracene induced mammary carcinoma in experimental rats--A dose-response study. J Cancer Res Ther. 2016 Jan-Mar;12(1):386-94.
11. Rae S, Spillane C, Blackshields G, Madden SF, Keenan J, Stordal B. The EMT-activator ZEB1 is unrelated to platinum drug resistance in ovarian cancer but is predictive of survival. Hum Cell. 2022 Sep;35(5):1547-1559.
12. Chen, Z., Zhang, W., Yun, Z., Zhang, X., Gong, F., Wang, Y. ... Leng, L. (2018). Ubiquitin‑like protein FAT10 regulates DNA damage repair via modification of proliferating cell nuclear antigen. Molecular Medicine Reports, 17, 7487-7496.
13. Safaee S, Fardi M, Hemmat N, Khosravi N, Derakhshani A, Silvestris N, Baradaran B. Silencing ZEB2 Induces Apoptosis and Reduces Viability in Glioblastoma Cell Lines. Molecules. 2021 Feb 9;26(4):901.
14. Rija, F. F., Hussein, S. Z., & Abdalla, M. A. (2021). Physiological and immunological disturbance in rheumatoid arthritis patients. Baghdad Sci J, 18(2), 247-252.
15. Ren, J., Wang, Y., Gao, Y., Mehta, S. B., & Lee, C. G. (2011). FAT10 mediates the effect of TNF-α in inducing chromosomal instability. Journal of cell science, 124(21), 3665-3675