"EFFECT OF VARIOUS FACTORS ON SEED GERMINATION STUDIES OF ZINNIA ELEGANS"
Main Article Content
Keywords
Cannabis, Cost-Beneit Analysis, Dietary Sucrose, Germination, Rhododendron santalum Seeds, Zinc Oxide, Plant Extracts Water
Abstract
The present studies were conducted to develop in vitro cost-effective micropropagation, ex vitro seed germination, and the effectiveness of green ZnO nanoparticles on Zinnia elegans. Cost-effective micropropagation was performed using three types of media. MS1 medium supplemented with (NAA 0.5mg/l and BA 1mg/l), MS2 medium supplemented with (NAA 0.5mg/l and BA 1.5 mg/l), and Isabgol cost-effective media supplemented with (0.5mg/l NAA and BA 1 mg/l) were used for in vitro establishment of the culture. Tap water was used instead of distilled water and table sugar instead of sucrose powder for production. It was observed that the production rate is slower than with standard MS media. Ex- vitro seed germination was done on the best six plants of Zinnia elegans. Data was noted for seed germination percentage, seed vigour, Max average shoot length, Max average root length, and Max average secondary root length. P-value, dry weight, and wet weight of an ex-vitro seed germination were noted down. Effects of green ZnO nanoparticles on Ex- vitro seed germination were obtained by using three plant extracts Rhododendron arboretum, Cannabis sativa, and Santalum album.
References
.Shah, A. H., Shah, S. H., Farooq, G., Sajad, M. A., & Khan, M. A. S. (2020). 19. Effects of natural growth regulators on micropropagation of potatoes. Pure appl. Biol. , 9(2). http:// dx.doi.org/10.19045/bspab.2020.90150 19.Sahoo, S., Sanghamitra, P., Nanda, N., Pawar, S., Pandit, E., Bastia, R., ... & Pradhan, S. K. (2020). Association of molecular markers with physio-biochemical traits related to seed vigour in rice. Physiol.Mol. Biol.Plants, 26, 1989-2003 .https://doi.org/10.1007/ s12298-020-00879-y 20.Salih, A. M., Qahtan, A. A., Al-Qurainy, F., & Al-Munqedhi, B. M. (2022). Impact of Biogenic Ag-Containing Nanoparticles on Germination Rate, Growth, Physiological, Biochemical Parameters, and Antioxidant System of Tomato (Solanum tuberosum L.) In Vitro. Processes, 10(5), 825. https://doi.org/10.3390/pr10050825 21.Thakur, N., Chrungoo, S., Rana, S., Kaur, S., Kaur, S., & Pathak, A. (2021). Effect Of Copper Nanoparticles On In-Vitro Seed Germination Of Wheat (Triticum Aestivum L.) Varieties. Ijpsr. Int. J. Pharmaceut. Sci. Res, 12(8), 4307-4313. http://dx.doi.org/10.13040/ IJPSR.0975- 8232.12(8).4307-13 22.Yao, N., Zheng, B., Wang, T., & Cao, X. (2023). Isolation of Tulasnella spp. from Cultivated Paphiopedilum Orchids and Screening of Germination-Enhancing Fungi. J. MFungi, 9(6). https://doi.org/10.3390/jof9060597 23.Zale, P. J., Clayton, A., Nix, J., & Taylor, M. (2022). Asymbiotic in vitro seed germination, in vitro seedling development, and ex vitro acclimatisation of Spiranthes. Appl. Plant Sci., 10(5), e11494.https://doi.org/10.1002/aps3.11494

