ANTI-FUNGAL EFFICACY OF MISWAK EXTRACT (SALVADORA PERSICA) AND COMMERCIAL CLEANER AGAINST CANDIDA ALBICANS ON HEATCURED PMMA DENTURE
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Keywords
Candida albicans, Commercial cleaners, Miswak, Acrylic resin
Abstract
Denture-induced stomatitis is the most common pathogenic reaction of denture-bearing mucosa, caused by Candida albicans. Miswak is naturally available oral hygiene tool and have been showed to have antifungal activity against Candida albicans in oral cavity.
Aim: The aim of the study was to compare the antifungal efficacy between commercial cleaner (Corega) and Miswak extract (Salvadora persica) against Candida albicans on heat cured Polymethyl methacrylate (PMMA) acrylic denture base resin.
Materials and methods: Forty-eight samples of heat cured PMMA acrylic denture base resin were fabircated in the study. The sterile acrylic resin specimens were immersed in standardized cell suspension of Candida albicans and incubated for 60 minutes at 370C for cell adhesion and 2 hours at 370C for biofilm formation. After 24 hours biofilm was evaluated by cell viability (CFUs) on SDA and cell counting of Candida albicans under light microscope at x400 magnification. The fungicidal effect of commercial cleaner and Miswak extract on Candida albicans biofilm was then evaluated by colony-forming units on SDA and cell counting under light microscope at x 400 magnification.
Results: Screening test agar disk-diffusion assay showed mean inhibitory zone of 3mm for commercial cleaner as compared to Miswak extract, which showed mean inhibitory zone of 2mm and 1mm for different concentrations. Broth microdilution method showed 31mg/ml MIC and 62.5mg/ml Minimal Fungicidal Concentration (MFC) values for commercial cleaner as compared to Miswak extract that showed 125mg/ml MIC and 250mg/ml MFC values against Candida albicans. A significant difference (p<0.05) was observed between pre and post treatment of both commercial cleaner and Miswak extract, for CFUs and cell count for Candida albicans.
Conclusion: Commercial denture cleaner (Corega) showed better antifungal (C albicans) activity than Miswak extract (Salvadora persica) on heat cured PMMA acrylic denture base resin.
References
Williams D, Silva SC, Malic S, Kuriyama T, Lewis MA. Candida biofilms and oral candidiasis: treatment and prevention. 2012.
Nawasrah A, AlNimr A, Ali AA. Antifungal effect of henna against Candida albicans adhered to acrylic resin as a possible method for prevention of denture stomatitis. International Journal of Environmental Research and Public Health. 2016;13(5):520.
Nikawa H, Yamamoto T, Hamada T, Sadamori S, Agrawal S. Cleansing efficacy of commercial denture cleansers: ability to reduce Candida albicans biofilm activity. International Journal of Prosthodontics. 1995;8(6).
Badaró MM, Bueno FL, Arnez RM, de Cássia Oliveira V, Macedo AP, de Souza RF, Paranhos HD, Silva-Lovato CH. The effects of three disinfection protocols on Candida spp., denture stomatitis, and biofilm: a parallel group randomized controlled trial. Journal of Prosthetic Dentistry. 2020;124(6):690–698.
Kumar MN, Thippeswamy H, Swamy KR, Gujjari AK. Efficacy of commercial and household denture cleansers against Candida albicans adherent to acrylic denture base resin: an in vitro study. Indian Journal of Dental Research. 2012;23(1):39.
Rokaya D, Kritsana J, Amornvit P, Dhakal N, Khurshid Z, Zafar MS, Saonanon P. Magnification of iris through clear acrylic resin in ocular prosthesis. Journal of Functional Biomaterials. 2022;13(1):29.
Almeida A. Photodynamic therapy in the inactivation of microorganisms. Antibiotics. 2020;9(4):138.
da Costa RM, Poluha RL, De la Torre Canales G, Junior JF, Conti PC, Neppelenbroek KH, Porto VC. The effectiveness of microwave disinfection in treating Candida-associated denture stomatitis: a systematic review and meta-analysis. Clinical Oral Investigations. 2020;24:3821–3832.
AlBin-Ameer MA, Alsrheed MY, Aldukhi IA, Matin A, Khan SQ, Abualsaud R, Gad MM. Effect of protective coating on surface properties and Candida albicans adhesion to denture base materials. Journal of Prosthodontics. 2020;29(1):80–86.
Meto A, Colombari B, Odorici A, Giva LB, Pericolini E, Regina AL, Blasi E. Antibacterial effects of MicroRepair® BIOMA-based toothpaste and chewing gum on orthodontic elastics contaminated in vitro with saliva from healthy donors: a pilot study. Applied Sciences. 2020;10(19):6721.
Ezoddini-Ardakani FJ. Efficacy of Miswak (Salvadora persica) in preventing dental caries. 2010;2(5):499.
Choudhry Z, Kazmi SM, Malik S, Mirani ZA, Ahmed S, Rehman Qazi FU, Ahmed MA, Alrabiah M, AlAali KA, Vohra F, Abduljabbar T. Bacterial disinfection of polymethyl methacrylate (PMMA) resin polymer using low-level microwave irradiation and denture cleaning agent. Applied Sciences. 2022;12(12):5860.
Kiatwarawut K, Rokaya D, Sirisoontorn I. Antimicrobial activity of various disinfectants to clean thermoplastic polymeric appliances in orthodontics. Polymers. 2022;14(11):2256.
Kellesarian SV, Abduljabbar T, Vohra F, Malmstrom H, Yunker M, Kellesarian TV, Romanos GE, Javed F. Efficacy of antimicrobial photodynamic therapy in the disinfection of acrylic denture surfaces: a systematic review. Photodiagnosis and Photodynamic Therapy. 2017;17:103–110.
Heboyan A, Zafar MS, Karobari MI, Tribst JP. Insights into polymeric materials for prosthodontics and dental implantology. Materials. 2022;15(15):5383.
Alzahrani KM, Alrabiah M, AlAali KA, Vohra F, Abduljabbar T. Fracture strength of Er:YAG laser-treated PMMA denture base polymer colonized with C. albicans, S. aureus, S. mutans, and E. coli. Photodiagnosis and Photodynamic Therapy. 2022;40:103074.
Wong DM, Cheng LY, Chow T, Clark RK. Effect of processing method on the dimensional accuracy and water sorption of acrylic resin dentures. Journal of Prosthetic Dentistry. 1999;81(3):300–304.
Kimoto S, Kobayashi N, Kobayashi K, Kawara M. Effect of bench cooling on the dimensional accuracy of heat-cured acrylic denture base material. Journal of Dentistry. 2005;33(1):57–63.
Espinel-Ingroff A, Chaturvedi V, Fothergill A, Rinaldi M. Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study. Journal of Clinical Microbiology. 2002;40(10):3776–3781.
Mohammed SG. Comparative study of in vitro antibacterial activity of miswak extracts and different toothpastes. American Journal of Agricultural and Biological Sciences. 2013;8(1):82–88.
Jayanti AT, Nasution A, Suyanto H, Bramantoro T. The content of active materials in miswak (Salvadora persica): an analytical study using Fourier-transform infrared spectroscopy and ultraviolet–visible spectrophotometer. Journal of International Oral Health. 2021;13(3):258–263.
Al-Bayaty FH, Al-Koubaisi AH, Ali NAW, Abdulla MA. Effect of mouthwash extracted from Salvadora persica (Miswak) on dental plaque formation: a clinical trial. Journal of Medicinal Plants Research. 2013;4(14):1446–1458.
Al-Ayed MSZ, Asaad AM, Qureshi MA, Attia HG, AlMarrani AH. Antibacterial activity of Salvadora persica L. (Miswak) extracts against multidrug-resistant bacterial clinical isolates. Evidence-Based Complementary and Alternative Medicine. 2016;2016.
Darmani H, Al-Hiyasat A, Elbetieha A, Alkofahi A. The effect of an extract of Salvadora persica (Meswak) on fertility of male and female mice. Phytomedicine. 2003;10(1):62–65.
da Silva RA, Bernardo LP, Moreno JML, Lara VS, Porto VC. Equisetum giganteum influences the ability of Candida albicans in forming biofilms over the denture acrylic resin surface. Pharmaceutical Biology. 2017;55(1):1698–1702.
Khatak M, Khatak S, Siddiqui A, Vasudeva N, Aggarwal A, Aggarwal P. Salvadora persica. Pharmacognosy Reviews. 2010;4(8):209–214.
Al-Bagieh N, Idowu A, Salako N. Effect of aqueous extract of miswak on the in vitro growth of Candida albicans. Microbios. 1994;80(323):107–113.
Al-Obaida MI, Al-Essa MA, Asiri AA, Al-Rahla AA. Effectiveness of a 20% Miswak extract against a mixture of Candida albicans and Enterococcus faecalis. Saudi Medical Journal. 2010;31(6):640–643.
Al-Bayati FA, Sulaiman KD. In vitro antimicrobial activity of Salvadora persica L. extracts against some isolated oral pathogens in Iraq. Turkish Journal of Biology. 2008;32(1):57–62.
Gouveia CL, Freire ICM, Leite MLA, Figueiredo RDA, Almeida LFD, Cavalcanti YW, et al. Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans. Revista de Odontologia da UNESP. 2014;43:137–142.
de Freitas Fernandes FS, Pereira-Cenci T, da Silva WJ, Ricomini Filho AP, Straioto FG, Cury AADB. Efficacy of denture cleansers on Candida spp. biofilm formed on polyamide and polymethyl methacrylate resins. Journal of Prosthetic Dentistry. 2011;105(1):51–58.
Sousa FACG, Paradella TC, Koga-Ito CY, Jorge AOC. Effect of sodium bicarbonate on Candida albicans adherence to thermally activated acrylic resin. Brazilian Oral Research. 2009;23:381–385.
Nalbant AD, Kalkanci A, Filiz B, Kustimur S. Effectiveness of different cleaning agents against the colonization of Candida spp. and the in vitro detection of the adherence of these yeast cells to denture acrylic surfaces. Yonsei Medical Journal. 2008;49(4):647–654.
Walczak K, Schierz G, Basche S, Petto C, Boening K, Wieckiewicz M. Antifungal and surface properties of chitosan-salts modified PMMA denture base material. Molecules. 2020;25(24):5899.
Mubaraki MQ, Moaleem MMA, Alzahrani AH, Shariff M, Alqahtani SM, Porwal A, Al-Sanabani FA, Bhandi S, Tribst JPM, Heboyan A, Patil S. Assessment of conventionally and digitally fabricated complete dentures: a comprehensive review. Materials. 2022;15(11):3868.
Mirchandani B, Zhou T, Heboyan A, Yodmongkol S, Buranawat B. Biomechanical aspects of various attachments for implant overdentures: a review. Polymers. 2021;13(19):3248.
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Dr Nadia Wajdan
Assistant Professor, Prosthodontics, Multan Medical and Dental College Multan
Dr Muhammad Abi Waqas
Assistant Professor, Science of Dental Materials, Multan Medical and Dental College Multan
Mohammad Sohaib Noor
Sr, Multan Medical and Dental College Multan
Muhammad Talal Rafiq
Student Multan Medical and Dental College Multan
Dr Zain Ul Abedeen
Senior Lecturer College LCMD Karachi
Dr Rubbab Zahra
FCPS Oral and Maxillofacial Surgery, Assistant Professor, Oral Medicine Avicenna Dental College Lahore
